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<?xml version="1.0" encoding="ISO-8859-1"?>

<!-- XML Authors: Corinne Maufrais and Nicolas Joly,                             -->
<!-- 'Biological Software and Databases' Group, Institut Pasteur, Paris.         -->
<!-- Distributed under LGPLv2 License. Please refer to the COPYING.LIB document. -->

<program>
  <head>
    <name>epestfind</name>
    <package>
      <name>EMBOSS</name>
      <version>6.3.1</version>
      <doc>
        <title>EMBOSS</title>
        <description>
          <text lang="en">European Molecular Biology Open Software Suite</text>
        </description>
        <authors>Rice,P. Longden,I. and Bleasby, A.</authors>
        <reference>EMBOSS: The European Molecular Biology Open Software Suite (2000)  Rice,P. Longden,I. and Bleasby, A. Trends in Genetics 16, (6) pp276--277</reference>
        <sourcelink>http://emboss.sourceforge.net/download</sourcelink>
        <homepagelink>http://emboss.sourceforge.net</homepagelink>
      </doc>
    </package>
    <doc>
      <title>epestfind</title>
      <description>
        <text lang="en">Finds PEST motifs as potential proteolytic cleavage sites</text>
      </description>
      <doclink>http://bioweb2.pasteur.fr/docs/EMBOSS/epestfind.html</doclink>
      <doclink>http://emboss.sourceforge.net/docs/themes</doclink>
    </doc>
    <category>sequence:protein:motifs</category>
    <command>epestfind</command>
  </head>

  <parameters>

    <paragraph>
      <name>e_input</name>
      <prompt lang="en">Input section</prompt>

      <parameters>

        <parameter issimple="1" ismandatory="1">
          <name>e_sequence</name>
          <prompt lang="en">sequence option</prompt>
          <type>
            <biotype>Protein</biotype>
            <datatype>
              <class>Sequence</class>
            </datatype>
            <dataFormat>EMBL</dataFormat>
            <dataFormat>FASTA</dataFormat>
            <dataFormat>GCG</dataFormat>
            <dataFormat>GENBANK</dataFormat>
            <dataFormat>NBRF</dataFormat>
            <dataFormat>CODATA</dataFormat>
            <dataFormat>RAW</dataFormat>
            <dataFormat>SWISSPROT</dataFormat>
            <dataFormat>GFF</dataFormat>
            <card>1,1</card>
          </type>
          <format>
            <code proglang="python">("", " -sequence=" + str(value))[value is not None]</code>
          </format>
          <argpos>1</argpos>
          <comment>
            <text lang="en">Protein sequence USA to be analysed.</text>
          </comment>
        </parameter>

        <parameter>
          <name>e_mwdata</name>
          <prompt lang="en">Molecular weights data file</prompt>
          <type>
            <datatype>
              <class>MolecularWeights</class>
              <superclass>AbstractText</superclass>
            </datatype>
          </type>
          <format>
            <code proglang="python">("", " -mwdata=" + str(value))[value is not None ]</code>
          </format>
          <argpos>2</argpos>
        </parameter>
      </parameters>
    </paragraph>

    <paragraph>
      <name>e_required</name>
      <prompt lang="en">Required section</prompt>

      <parameters>

        <parameter issimple="1" ismandatory="1">
          <name>e_window</name>
          <prompt lang="en">Window length (value greater than or equal to 2)</prompt>
          <type>
            <datatype>
              <class>Integer</class>
            </datatype>
          </type>
          <vdef>
            <value>10</value>
          </vdef>
          <format>
            <code proglang="python">("", " -window=" + str(value))[value is not None and value!=vdef]</code>
          </format>
          <ctrl>
            <message>
              <text lang="en">Value greater than or equal to 2 is required</text>
            </message>
            <code proglang="python">value &gt;= 2</code>
          </ctrl>
          <argpos>3</argpos>
          <comment>
            <text lang="en">Minimal distance between positively charged amino acids.</text>
          </comment>
        </parameter>

        <parameter issimple="1" ismandatory="1">
          <name>e_order</name>
          <prompt lang="en">Sort order of results</prompt>
          <type>
            <datatype>
              <class>Choice</class>
            </datatype>
          </type>
          <vdef>
            <value>3</value>
          </vdef>
          <vlist>
            <velem>
              <value>1</value>
              <label>Length</label>
            </velem>
            <velem>
              <value>2</value>
              <label>Position</label>
            </velem>
            <velem>
              <value>3</value>
              <label>Score</label>
            </velem>
          </vlist>
          <format>
            <code proglang="python">("", " -order=" + str(value))[value is not None and value!=vdef]</code>
          </format>
          <argpos>4</argpos>
          <comment>
            <text lang="en">Name of the output file which holds the results of the analysis. Results may be sorted by length, position and score.</text>
          </comment>
        </parameter>
      </parameters>
    </paragraph>

    <paragraph>
      <name>e_additional</name>
      <prompt lang="en">Additional section</prompt>

      <parameters>

        <parameter>
          <name>e_threshold</name>
          <prompt lang="en">Threshold score (value from -55.0 to +55.0)</prompt>
          <type>
            <datatype>
              <class>Float</class>
            </datatype>
          </type>
          <vdef>
            <value>+5.0</value>
          </vdef>
          <format>
            <code proglang="python">("", " -threshold=" + str(value))[value is not None and value!=vdef]</code>
          </format>
          <ctrl>
            <message>
              <text lang="en">Value greater than or equal to -55.0 is required</text>
            </message>
            <code proglang="python">value &gt;= -55.0</code>
          </ctrl>
          <ctrl>
            <message>
              <text lang="en">Value less than or equal to +55.0 is required</text>
            </message>
            <code proglang="python">value &lt;= +55.0</code>
          </ctrl>
          <argpos>5</argpos>
          <comment>
            <text lang="en">Threshold value to discriminate weak from potential PEST motifs. Valid PEST motifs are discriminated into 'poor' and  'potential' motifs depending on this threshold score. By default,  the default value is set to +5.0 based on experimental data.  Alterations are not recommended since significance is a matter of  biology, not mathematics.</text>
          </comment>
        </parameter>
      </parameters>
    </paragraph>

    <paragraph>
      <name>e_advanced</name>
      <prompt lang="en">Advanced section</prompt>

      <parameters>

        <parameter>
          <name>e_mono</name>
          <prompt lang="en">Use monoisotopic weights</prompt>
          <type>
            <datatype>
              <class>Boolean</class>
            </datatype>
          </type>
          <vdef>
            <value>0</value>
          </vdef>
          <format>
            <code proglang="python">("", " -mono")[ bool(value) ]</code>
          </format>
          <argpos>6</argpos>
        </parameter>

        <parameter>
          <name>e_potential</name>
          <prompt lang="en">Display potential pest motifs</prompt>
          <type>
            <datatype>
              <class>Boolean</class>
            </datatype>
          </type>
          <vdef>
            <value>1</value>
          </vdef>
          <format>
            <code proglang="python">(" -nopotential", "")[ bool(value) ]</code>
          </format>
          <argpos>7</argpos>
          <comment>
            <text lang="en">Decide whether potential PEST motifs should be printed.</text>
          </comment>
        </parameter>

        <parameter>
          <name>e_poor</name>
          <prompt lang="en">Display poor pest motifs</prompt>
          <type>
            <datatype>
              <class>Boolean</class>
            </datatype>
          </type>
          <vdef>
            <value>1</value>
          </vdef>
          <format>
            <code proglang="python">(" -nopoor", "")[ bool(value) ]</code>
          </format>
          <argpos>8</argpos>
          <comment>
            <text lang="en">Decide whether poor PEST motifs should be printed.</text>
          </comment>
        </parameter>

        <parameter>
          <name>e_invalid</name>
          <prompt lang="en">Display invalid pest motifs</prompt>
          <type>
            <datatype>
              <class>Boolean</class>
            </datatype>
          </type>
          <vdef>
            <value>0</value>
          </vdef>
          <format>
            <code proglang="python">("", " -invalid")[ bool(value) ]</code>
          </format>
          <argpos>9</argpos>
          <comment>
            <text lang="en">Decide whether invalid PEST motifs should be printed.</text>
          </comment>
        </parameter>

        <parameter>
          <name>e_map</name>
          <prompt lang="en">Display pest motifs map</prompt>
          <type>
            <datatype>
              <class>Boolean</class>
            </datatype>
          </type>
          <vdef>
            <value>1</value>
          </vdef>
          <format>
            <code proglang="python">(" -nomap", "")[ bool(value) ]</code>
          </format>
          <argpos>10</argpos>
          <comment>
            <text lang="en">Decide whether PEST motifs should be mapped to sequence.</text>
          </comment>
        </parameter>
      </parameters>
    </paragraph>

    <paragraph>
      <name>e_output</name>
      <prompt lang="en">Output section</prompt>

      <parameters>

        <parameter>
          <name>e_outfile</name>
          <prompt lang="en">Name of the output file (e_outfile)</prompt>
          <type>
            <datatype>
              <class>Filename</class>
            </datatype>
          </type>
          <vdef>
            <value>epestfind.e_outfile</value>
          </vdef>
          <format>
            <code proglang="python">("" , " -outfile=" + str(value))[value is not None]</code>
          </format>
          <argpos>11</argpos>
          <comment>
            <text lang="en">Name of file to which results will be written.</text>
          </comment>
        </parameter>

        <parameter isout="1">
          <name>e_outfile_out</name>
          <prompt lang="en">outfile_out option</prompt>
          <type>
            <datatype>
              <class>EpestfindReport</class>
              <superclass>Report</superclass>
            </datatype>
          </type>
          <filenames>
            <code proglang="python">e_outfile</code>
          </filenames>
        </parameter>

        <parameter issimple="1" ismandatory="1">
          <name>e_graph</name>
          <prompt lang="en">Choose the e_graph output format</prompt>
          <type>
            <datatype>
              <class>Choice</class>
            </datatype>
          </type>
          <vdef>
            <value>png</value>
          </vdef>
          <vlist>
            <velem>
              <value>png</value>
              <label>Png</label>
            </velem>
            <velem>
              <value>gif</value>
              <label>Gif</label>
            </velem>
            <velem>
              <value>cps</value>
              <label>Cps</label>
            </velem>
            <velem>
              <value>ps</value>
              <label>Ps</label>
            </velem>
            <velem>
              <value>meta</value>
              <label>Meta</label>
            </velem>
            <velem>
              <value>data</value>
              <label>Data</label>
            </velem>
          </vlist>
          <format>
            <code proglang="python">(" -graph=" + str(vdef), " -graph=" + str(value))[value is not None and value!=vdef]</code>
          </format>
          <argpos>12</argpos>
        </parameter>

        <parameter>
          <name>xy_goutfile</name>
          <prompt lang="en">Name of the output graph</prompt>
          <type>
            <datatype>
              <class>Filename</class>
            </datatype>
          </type>
          <vdef>
            <value>epestfind_xygraph</value>
          </vdef>
          <format>
            <code proglang="python">("" , " -goutfile=" + str(value))[value is not None]</code>
          </format>
          <argpos>13</argpos>
        </parameter>

        <parameter isout="1">
          <name>xy_outgraph_png</name>
          <prompt lang="en">Graph file</prompt>
          <type>
            <datatype>
              <class>Picture</class>
              <superclass>Binary</superclass>
            </datatype>
          </type>
          <precond>
            <code proglang="python">e_graph == "png"</code>
          </precond>
          <filenames>
            <code proglang="python">"*.png"</code>
          </filenames>
        </parameter>

        <parameter isout="1">
          <name>xy_outgraph_gif</name>
          <prompt lang="en">Graph file</prompt>
          <type>
            <datatype>
              <class>Picture</class>
              <superclass>Binary</superclass>
            </datatype>
          </type>
          <precond>
            <code proglang="python">e_graph == "gif"</code>
          </precond>
          <filenames>
            <code proglang="python">"*.gif"</code>
          </filenames>
        </parameter>

        <parameter isout="1">
          <name>xy_outgraph_ps</name>
          <prompt lang="en">Graph file</prompt>
          <type>
            <datatype>
              <class>PostScript</class>
              <superclass>Binary</superclass>
            </datatype>
          </type>
          <precond>
            <code proglang="python">e_graph == "ps" or e_graph == "cps"</code>
          </precond>
          <filenames>
            <code proglang="python">"*.ps"</code>
          </filenames>
        </parameter>

        <parameter isout="1">
          <name>xy_outgraph_meta</name>
          <prompt lang="en">Graph file</prompt>
          <type>
            <datatype>
              <class>Picture</class>
              <superclass>Binary</superclass>
            </datatype>
          </type>
          <precond>
            <code proglang="python">e_graph == "meta"</code>
          </precond>
          <filenames>
            <code proglang="python">"*.meta"</code>
          </filenames>
        </parameter>

        <parameter isout="1">
          <name>xy_outgraph_data</name>
          <prompt lang="en">Graph file</prompt>
          <type>
            <datatype>
              <class>Text</class>
            </datatype>
          </type>
          <precond>
            <code proglang="python">e_graph == "data"</code>
          </precond>
          <filenames>
            <code proglang="python">"*.dat"</code>
          </filenames>
        </parameter>
      </parameters>
    </paragraph>

    <parameter ishidden="1">
      <name>auto</name>
      <prompt lang="en">Turn off any prompting</prompt>
      <type>
        <datatype>
          <class>String</class>
        </datatype>
      </type>
      <format>
        <code proglang="python">" -auto -stdout"</code>
      </format>
      <argpos>14</argpos>
    </parameter>
  </parameters>
</program>