/usr/share/perl5/Bio/Tools/Run/Genscan.pm is in libbio-perl-run-perl 1.7.1-3.
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#
# Please direct questions and support issues to <bioperl-l@bioperl.org>
#
# Cared for by
#
# Copyright Balamurugan Kumarasamy
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
=head1 NAME
Bio::Tools::Run::Genscan - Object for identifying genes in a
given sequence given a matrix(for appropriate organisms).
=head1 SYNOPSIS
# Build a Genscan factory
my $param = ('MATRIX'=>HumanIso.smat);
my $factory = Bio::Tools::Run::Genscan->new($param);
# Pass the factory a Bio::Seq object
#@genes is an array of Bio::Tools::Predictions::Gene objects
my @genes = $factory->run($seq);
=head1 DESCRIPTION
Genscan is a gene identifying program developed by Christopher Burge
http://genes.mit.edu/burgelab/
By default it looks for an executable called I<genscan> and data/parameter files
in the directory specified by the I<GENSCANDIR> environmental variable.
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Support
Please direct usage questions or support issues to the mailing list:
I<bioperl-l@bioperl.org>
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:
http://redmine.open-bio.org/projects/bioperl/
=head1 AUTHOR - Bala
Email savikalpa@fugu-sg.org
=head1 APPENDIX
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
=cut
package Bio::Tools::Run::Genscan;
use vars qw($AUTOLOAD @ISA $PROGRAM $PROGRAMDIR
$PROGRAMNAME @GENSCAN_PARAMS %OK_FIELD);
use strict;
use Bio::Seq;
use Bio::SeqIO;
use Bio::Root::Root;
use Bio::Root::IO;
use Bio::Factory::ApplicationFactoryI;
use Bio::Tools::Genscan;
use Bio::Tools::Run::WrapperBase;
@ISA = qw(Bio::Root::Root Bio::Tools::Run::WrapperBase);
BEGIN {
@GENSCAN_PARAMS=qw(MATRIX VERBOSE QUIET);
foreach my $attr ( @GENSCAN_PARAMS)
{ $OK_FIELD{$attr}++; }
}
=head2 program_name
Title : program_name
Usage : $factory>program_name()
Function: holds the program name
Returns: string
Args : None
=cut
sub program_name {
return 'genscan';
}
=head2 program_dir
Title : program_dir
Usage : $factory->program_dir(@params)
Function: returns the program directory, obtained from ENV variable.
Returns: string
Args :
=cut
sub program_dir {
return Bio::Root::IO->catfile($ENV{GENSCANDIR});
}
sub AUTOLOAD {
my $self = shift;
my $attr = $AUTOLOAD;
$attr =~ s/.*:://;
$attr = uc $attr;
$self->throw("Unallowed parameter: $attr !") unless $OK_FIELD{$attr};
$self->{$attr} = shift if @_;
return $self->{$attr};
}
sub new {
my ($class,@args) = @_;
my $self = $class->SUPER::new(@args);
my ($attr, $value);
while (@args) {
$attr = shift @args;
$value = shift @args;
next if( $attr =~ /^-/ ); # don't want named parameters
$self->$attr($value);
}
return $self;
}
=head2 predict_genes()
Title : predict_genes()
Usage : DEPRECATED: use $obj->run($seq) instead
Function: Runs genscan and creates an array of Genes
Returns : An array of Bio::Tools::Prediction::Gene objects
Args : A Bio::PrimarySeqI
=cut
sub predict_genes{
return shift->run(@_);
}
=head2 run
Title : run
Usage : $obj->run($seq)
Function: Runs genscan and creates an array of Genes
Returns : An array of Bio::Tools::Prediction::Gene objects
Args : A Bio::PrimarySeqI
=cut
sub run {
my ($self,$seq) = @_;
my $infile1 = $self->_writeSeqFile($seq);
$self->_set_input($infile1);
my @feat = $self->_run();
return @feat;
}
=head2 _run
Title : _run
Usage : $obj->_run()
Function: Internal(not to be used directly)
Returns : An array of Bio::Tools::Prediction::Gene objects
Args :
=cut
sub _run {
my ($self) = @_;
my @genes;
my $gene;
my $str = $self->executable.' '.$self->MATRIX.' '.$self->{'input'};
if($self->verbose){
$str.=" -v ";
}
if($self->quiet){
my $null = ($^O =~ m/mswin/i) ? 'NUL' : '/dev/null';
open(STDERR,">$null");
}
unless (open(GENSCAN, "$str |")){
$self->warn("Cannot run $str");
}
close(STDERR);
my $genScanParser = Bio::Tools::Genscan->new(-fh=> \*GENSCAN);
while( $gene = $genScanParser->next_prediction()){
push(@genes, $gene);
}
$self->cleanup();
return @genes;
}
=head2 _set_input()
Title : _set_input
Usage : obj->_set_input($matrixFile,$seqFile)
Function: Internal(not to be used directly)
Returns :
Args :
=cut
sub _set_input() {
my ($self,$infile1) = @_;
$self->{'input'}=$infile1;
}
=head2 _writeSeqFile()
Title : _writeSeqFile
Usage : obj->_writeSeqFile($seq)
Function: Internal(not to be used directly)
Returns :
Args :
=cut
sub _writeSeqFile(){
my ($self,$seq) = @_;
my ($tfh,$inputfile) = $self->io->tempfile(-dir=>$self->tempdir);
my $in = Bio::SeqIO->new(-fh => $tfh , '-format' => 'fasta');
$in->write_seq($seq);
$in->close();
close($tfh);
undef $tfh;
return $inputfile;
}
1;
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