/usr/share/staden/etc/gap5rc is in staden-common 2.0.0+b11-2.
This file is owned by root:root, with mode 0o644.
The actual contents of the file can be viewed below.
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# Packages required
#-----------------------------------------------------------------------------
load_package tk_utils
#if {[info commands tk] != ""} {
# package require Iwidgets
#}
# Next line can be removed when using the updated newgap_top.tcl
# load_package %L/gap {} gap 1
#-----------------------------------------------------------------------------
# Global variables
#-----------------------------------------------------------------------------
set_def CONSENSUS_MODE 2
set_def CONSENSUS_CUTOFF 0.02
set_def QUALITY_CUTOFF 0
set_def CHEM_AS_DOUBLE 0
set_def CONSENSUS_IUB 0
set_def ERROR_SCROLL 1
set_def DIFF_WARNING 20
set_def LOGGING 1
set_def MAX_MENU_ITEMS 40
set_def CHECKDB_AT_STARTUP 0
set_def ALIGNMENT.OPEN.COST 8
set_def ALIGNMENT.EXTEND.COST 7
set_def ALIGNMENT.MATRIX_FILE $env(STADTABL)/nuc_matrix
set_def MENU_LEVEL b
set_def GENETIC_CODE_DIR $env(STADTABL)/gcodes
set_def GENETIC_CODE $env(STADTABL)/gcodes/code_1
set_def TEMPLATE_TOLERANCE 1.0
set_def MIN_VECTOR_LENGTH 5
set_def IGNORE_ALL_PTYPE 0
set_def IGNORE_CUSTOM_PTYPE 0
#-----------------------------------------------------------------------------
# Colours
#-----------------------------------------------------------------------------
set_def BACKGROUNDS ""
# If you want Gap4 to use different colours for subsequent gap4s that
# start up, add them as a list. Eg:
#set_def BACKGROUNDS "#c8bfff #c8bf98 #c6f3ad #dbc6c2 #ffffa4 #add6da #c1edf8 #b8cb7d #a8a8a4 #e8db88 bisque #ffa0a0"
set_def CONTIG_SEL.CURSOR_COLOUR "blue"
set_def CONTIG_SEL.CROSSHAIRS_COLOUR "black"
set_def CONTIG_SEL.CONTIG_COLOUR "black"
set_def CONTIG_SEL.HIGHLIGHT_COLOUR "hotpink"
set_def STOP_CODON.TICK_COLOUR "black"
set_def STOP_CODON.RULER_COLOUR "black"
set_def STOP_CODON.TEXT_COLOUR "black"
set_def TEMPLATE.CROSSHAIR_COLOUR "black"
set_def TEMPLATE.EDITOR.CROSSHAIR_COLOUR0 "blue"
set_def TEMPLATE.EDITOR.CROSSHAIR_COLOUR1 "orange4"
set_def TEMPLATE.EDITOR.CROSSHAIR_COLOUR2 "green3"
set_def TEMPLATE.EDITOR.CROSSHAIR_COLOUR3 "red"
set_def TEMPLATE.EDITOR.CROSSHAIR_COLOUR4 "hotpink"
set_def TEMPLATE.R_ENZ.CUTS_COLOUR "black"
set_def TEMPLATE.FORW_REV_COLOUR "#ff5968"
set_def TEMPLATE.CONTRADICT_COLOUR "black"
set_def TEMPLATE.DIFF_CONTIGS_COLOUR "green"
set_def TEMPLATE.ONE_READING_COLOUR "blue"
set_def TEMPLATE.PRIMER_UNKNOWN_COLOUR "red"
set_def TEMPLATE.PRIMER_FORWARD_COLOUR "green3"
set_def TEMPLATE.PRIMER_REVERSE_COLOUR "orange"
set_def TEMPLATE.PRIMER_DIFF_CONTIGS_COLOUR "purple"
set_def TEMPLATE.PRIMER_CUSTOM_FOR_COLOUR "cyan4"
set_def TEMPLATE.PRIMER_CUSTOM_REV_COLOUR "orangered"
set_def TEMPLATE.SPAN_CONTIG_COLOUR "yellow"
set_def TEMPLATE.SPAN_CONTIG_INCONS_COLOUR "yellow3"
set_def TEMPLATE.RULER_COLOUR "black"
set_def TEMPLATE.HIGHLIGHT_COLOUR "white"
# MARGIN1 is the percentage distance from edge to auto-scroll when visible
# MARGIN2 is the percentage distance from edge after auto-scroll when not visible
set_def TEMPLATE.SCROLL_MARGIN1 10
set_def TEMPLATE.SCROLL_MARGIN2 30
set_def TAG_BRIEF_FORMAT \
{Tag type:%t Dir:%d Comment:"%.100c"}
#set_def READ_BRIEF_FORMAT \
# {Reading:%n(#%Rn) Length:%l(%L) Vector:%Tv Clone:%Cn Chemistry:%a Primer:%P Tstat:%Tc %c}
#set_def READ_BRIEF_FORMAT \
# {%n(#%Rn) Clone:%Cn Vector:%Tv Type:%P;%a Tmpl:%Tc %c}
set_def READ_BRIEF_FORMAT \
{Reading:%n(#%Rn) Tech:%V Length:%l(%L) MappingQ:%m%**/%*m Pos:%d%S%p / %*d%*S%*p}
set_def CONTIG_BRIEF_FORMAT \
{Contig:%n(#%Rn) Length:%l Start:%s End:%e}
set_def BASE_BRIEF_FORMAT1 \
{ Base %b confidence:%4.1c (Prob. %Rc, raw %4.1A %4.1C %4.1G %4.1T) Position %Rp %n}
set_def BASE_BRIEF_FORMAT2 \
{Base confidence:%4.1c (Prob. %Rc) A=%4.1A C=%4.1C G=%4.1G T=%4.1T *=%4.1* / %h=%4.1H Depth %d Pos %p (%r ref)}
set_def CONTIG_EDITOR.AUTO_FOCUS 0
set_def CONTIG_EDITOR.LIST_CONFIDENCE.ONLY_NUM_ERRS 0
set_def CONTIG_EDITOR.CUTOFF_COLOUR grey70
set_def CONTIG_EDITOR.BASE_COLOUR black
set_def CONTIG_EDITOR.AUTO_DISPLAY_TRACES 0
set_def CONTIG_EDITOR.DISPLAY_MINI_TRACES 0
set_def CONTIG_EDITOR.AUTO_SAVE 0
set_def CONTIG_EDITOR.AMINO_ACID_MODE 0
set_def CONTIG_EDITOR.GROUP_BY 0
set_def CONTIG_EDITOR.SHOW_QUALITY 0
set_def CONTIG_EDITOR.SHOW_MAPPING_QUALITY 1
set_def CONTIG_EDITOR.SHOW_CUTOFFS 0
set_def CONTIG_EDITOR.POS_TYPE P; # (P)added, (R)eference
set_def CONTIG_EDITOR.SHOW_CONSENSUS_QUALITY 0
set_def CONTIG_EDITOR.MOVE_ON_EDITS 1
set_def CONTIG_EDITOR.SHOW_UNPADDED 0
set_def CONTIG_EDITOR.TEMPLATE_NAMES 0
#set_def CONTIG_EDITOR.FONT {9x15Bold}
#set_def CONTIG_EDITOR.FONT_MB {-*-helvetica-*-r-*-*-*-100-*-*-*-*-*-*}
set_def CONTIG_EDITOR.FONT {sheet_font}
set_def CONTIG_EDITOR.FONT_MB {menu_font}
set_def CONTIG_EDITOR.MAX_HEIGHT 16
set_def CONTIG_EDITOR.SEQ_WIDTH 80
set_def CONTIG_EDITOR.NAMES_WIDTH 11
set_def CONTIG_EDITOR.NAMES_XPOS 0
set_def CONTIG_EDITOR.MACRO_AUTOEDIT 0
set_def CONTIG_EDITOR.TAG_POPUPS 0
set_def CONTIG_EDITOR.SE_INS_ANY 0
set_def CONTIG_EDITOR.SE_DEL_READ 0
set_def CONTIG_EDITOR.SE_INS_CONS 0
set_def CONTIG_EDITOR.SE_DEL_DASH_CONS 1
set_def CONTIG_EDITOR.SE_DEL_ANY_CONS 0
set_def CONTIG_EDITOR.SE_REPLACE_CONS 0
set_def CONTIG_EDITOR.SE_READ_SHIFT 0
set_def CONTIG_EDITOR.SE_TRANS_ANY 0
set_def CONTIG_EDITOR.SE_UPPERCASE 1
set_def CONTIG_EDITOR.SE_EDIT_MODE 0
set_def CONTIG_EDITOR.SE_SET.1 {0 0 0 1 0 0 0 0 1 1}
set_def CONTIG_EDITOR.SE_SET.2 {1 1 1 1 0 1 1 0 1 1}
set_def CONTIG_EDITOR.SE_FAST_DELETE_ANNOTATION 0
set_def CONTIG_EDITOR.INSERTION_CONFIDENCE 100
set_def CONTIG_EDITOR.REPLACE_CONFIDENCE 100
set_def CONTIG_EDITOR.TRACE_DIFF 0
set_def CONTIG_EDITOR.TRACE_CONS_MATCH 1
set_def CONTIG_EDITOR.TRACE_CONS_SELECT 1
set_def CONTIG_EDITOR.TRACE_DIFF_ALGORITHM 0
set_def CONTIG_EDITOR.TRACE_DIFF_SCALE 1
set_def CONTIG_EDITOR.TRACE_LOCK 1
set_def CONTIG_EDITOR.PACK_SEQUENCES 1
set_def CONTIG_EDITOR.GROUP_BY_PRIMARY 1
set_def CONTIG_EDITOR.GROUP_BY_SECONDARY 1
set_def CONTIG_EDITOR.GROUP_PRIMARY 1
set_def CONTIG_EDITOR.GROUP_SECONDARY 3
set_def CONTIG_EDITOR.DISAGREEMENTS 0
set_def CONTIG_EDITOR.DISAGREE_MODE 1
set_def CONTIG_EDITOR.DISAGREE_CASE 1
set_def CONTIG_EDITOR.DISAGREE_QUAL 0
set_def CONTIG_EDITOR.DIFF1_BG "#c0c0f0"
set_def CONTIG_EDITOR.DIFF2_BG "#8080ff"
set_def CONTIG_EDITOR.DIFF1_FG "#2020c0"
set_def CONTIG_EDITOR.DIFF2_FG "#4040ff"
set_def CONTIG_EDITOR.COMPARE_STRANDS 0
set_def CONTIG_EDITOR.SHOW_EDITS 0
set_def CONTIG_EDITOR.EDIT_DEL_COLOUR "red"
set_def CONTIG_EDITOR.EDIT_BASE_COLOUR "#ff9090"
set_def CONTIG_EDITOR.EDIT_PAD_COLOUR "#90ee90"
set_def CONTIG_EDITOR.EDIT_CONF_COLOUR "#6060ff"
set_def CONTIG_EDITOR.TEMP_DIST_COLOUR "#bbbbbb"
set_def CONTIG_EDITOR.TEMP_STRAND_COLOUR "#FF3939"
set_def CONTIG_EDITOR.TEMP_PRIM_COLOUR "#5959FF"
set_def CONTIG_EDITOR.TEMP_OTHER_COLOUR "#555555"
set_def CONTIG_EDITOR.TEMP_END_COLOUR "#dddddd"
set_def CONTIG_EDITOR.TEMP_ENDSPAN_COLOUR "#dd55dd"
#set_def CONTIG_EDITOR.QUAL0_COLOUR "#494949"
#set_def CONTIG_EDITOR.QUAL1_COLOUR "#696969"
#set_def CONTIG_EDITOR.QUAL2_COLOUR "#898989"
#set_def CONTIG_EDITOR.QUAL3_COLOUR "#999999"
#set_def CONTIG_EDITOR.QUAL4_COLOUR "#a9a9a9"
#set_def CONTIG_EDITOR.QUAL5_COLOUR "#b8b8b8"
#set_def CONTIG_EDITOR.QUAL6_COLOUR "#c0c0c0"
#set_def CONTIG_EDITOR.QUAL7_COLOUR "#c8c8c8"
#set_def CONTIG_EDITOR.QUAL8_COLOUR "#d0d0d0"
#set_def CONTIG_EDITOR.QUAL9_COLOUR "#d8d8d8"
# Better for phred values
set_def CONTIG_EDITOR.QUAL0_COLOUR "#494949"
set_def CONTIG_EDITOR.QUAL1_COLOUR "#696969"
set_def CONTIG_EDITOR.QUAL2_COLOUR "#898989"
set_def CONTIG_EDITOR.QUAL3_COLOUR "#a9a9a9"
set_def CONTIG_EDITOR.QUAL4_COLOUR "#b9b9b9"
set_def CONTIG_EDITOR.QUAL5_COLOUR "#c9c9c9"
set_def CONTIG_EDITOR.QUAL6_COLOUR "#d9d9d9"
set_def CONTIG_EDITOR.QUAL7_COLOUR "#e0e0e0"
set_def CONTIG_EDITOR.QUAL8_COLOUR "#e8e8e8"
set_def CONTIG_EDITOR.QUAL9_COLOUR "#f0f0f0"
set_def CONTIG_EDITOR.STRIPE_BG "#e0ece0"
set_def CONTIG_EDITOR.STRIPE_SIZE 0
set_def CONTIG_EDITOR.INDEL_COLOUR "#2050ff"
set_def CONTIG_EDITOR.QUAL_IGNORE "#ff5050"
set_def NAVIGATION.SELECT_COLOUR "bisque"
set_def CONTIG_EDITOR.SEARCH.POSITION_DEF ""
set_def CONTIG_EDITOR.SEARCH.UPOSITION_DEF ""
set_def CONTIG_EDITOR.SEARCH.ANNOTATION_DEF ""
set_def CONTIG_EDITOR.SEARCH.SEQUENCE_DEF "{} 0"
set_def CONTIG_EDITOR.SEARCH.CONSENSUS_DEF "{} 0"
set_def CONTIG_EDITOR.SEARCH.CONSQUALITY_DEF 30
set_def CONTIG_EDITOR.SEARCH.DEPTH_LT_DEF 5
set_def CONTIG_EDITOR.SEARCH.DEPTH_GT_DEF 100
set_def CONTIG_EDITOR.SEARCH.FILE_DEF ""
set_def CONTIG_EDITOR.SEARCH.NAME_DEF ""
set_def CONTIG_EDITOR.SEARCH.DISCREPANCY_DEF 25
set_def CONTIG_EDITOR.SEARCH.CONSHET_DEF 10
set_def CONTIG_EDITOR.SEARCH.CONSDISCREP_DEF 2.0
set_def CONTIG_EDITOR.SEARCH.TAG_DEF COMM
set_def CONTIG_EDITOR.SEARCH.AUTO_EDIT_TAG 0
# Default search type.
# Choose from position, uposition, problem, anno, sequence, quality,
# consquality, file, name, edit, verifyand, verifyor, discrepancy, tag.
set_def CONTIG_EDITOR.SEARCH.DEFAULT_TYPE consquality
set_def CONTIG_EDITOR.SEARCH.DEFAULT_DIRECTION forward
set_def CONTIG_EDITOR.SEARCH.DEFAULT_STRAND +
set_def CONTIG_EDITOR.SEARCH.DEFAULT_WHERE 3
set_def CONTIG_EDITOR.STATUS_STRAND 0
set_def CONTIG_EDITOR.STATUS_FRAME1P 0
set_def CONTIG_EDITOR.STATUS_FRAME2P 0
set_def CONTIG_EDITOR.STATUS_FRAME3P 0
set_def CONTIG_EDITOR.STATUS_FRAME1M 0
set_def CONTIG_EDITOR.STATUS_FRAME2M 0
set_def CONTIG_EDITOR.STATUS_FRAME3M 0
set_def CONTIG_EDITOR.STATUS_AUTO_TRANSLATE 0
# This controls whether the Delete key deletes to the right, as in Motif
# and Windows styles, or deletes to the left like Backspace.
if {"$tcl_platform(platform)" == "windows"} {
set_def CONTIG_EDITOR.DELETE_RIGHT 1
} else {
set_def CONTIG_EDITOR.DELETE_RIGHT 0
}
# These control whether the undo information is stored by default. If you
# routinely align enormous contigs in the join editor then you may wish to
# disable storing the undo information (as this can consume LOTS of memory).
set_def CONTIG_EDITOR.STORE_UNDO 1
set_def CONTIG_EDITOR.MUTATIONS_TAGGED 1
set_def CONTIG_EDITOR.MUTATIONS_SORT 1
set_def CONTIG_EDITOR.MUTATIONS_MINQUAL 20
set_def JOIN_EDITOR.STORE_UNDO 1
set_def CONTIG_EDITOR.MOUSEWHEEL_SPEED 4
# Modifying these will control when strip pads thinks an "entire column of
# pads" really is one. This allows it to be fooled into deleted columns that
# are almost pads. It's definition is - calculate the consensus and if it's a
# pad then it's an entire column. The consensus_mode 0 (frequency) and cutoff
# 1 (100%) are the defaults, which therefore really do mean entirely pads.
set_def CONTIG_EDITOR.STRIP_PADS.CONSENSUS_MODE 0
set_def CONTIG_EDITOR.STRIP_PADS.CONSENSUS_CUTOFF 1
set_def TRACE_DISPLAY.CUTOFF_COLOUR grey70
set_def TRACE_DISPLAY.BACKGROUND white
set_def TRACE_DISPLAY.XMAG 150
set_def TRACE_DISPLAY.DIRECTION bottom
set_def TRACE_DISPLAY.TRACE_HEIGHT 150
set_def TRACE_DISPLAY.ROWS 5
set_def TRACE_DISPLAY.COLUMNS 1
set_def TRACE_DISPLAY.WINDOW_WIDTH 1000
set_def TRACE_DISPLAY.FULL_NAME 1
set_def TRACE_DISPLAY.SHOW_CONFIDENCE 0
set_def TRACE_DISPLAY.COMPACT_MODE 1
set_def TRACE_DISPLAY.LINE_WIDTH 0
# This defines the type, number, and order of traces shown when "auto-display
# traces" is enabled and a problem is searched for.
# The format is a space separated list of trace types. The valid types are:
#
# + Best +ve strand trace (any) agreeing with consensus
# +p Best +ve strand dye-primer trace agreeing with consensus
# +t Best +ve strand dye-terminator trace agreeing with consensus
# - Best -ve strand trace (any) agreeing with consensus
# -p Best -ve strand dye-primer trace agreeing with consensus
# -t Best -ve strand dye-terminator trace agreeing with consensus
# d Best trace disagreeing with consensus
# +d Best +ve strand trace disagreeing with consensus
# -d Best -ve strand trace disagreeing with consensus
# +2 2nd best +ve strand trace (any) agreeing with consensus
# +2p 2nd best +ve strand dye-primer trace agreeing with consensus
# +2t 2nd best +ve strand dye-terminator trace agreeing with consensus
# -2 2nd best -ve strand trace (any) agreeing with consensus
# -2p 2nd best -ve strand dye-primer trace agreeing with consensus
# -2t 2nd best -ve strand dye-terminator trace agreeing with consensus
# 2d 2nd best trace disagreeing with consensus
# +2d 2nd best +ve strand trace disagreeing with consensus
# -2d 2nd best -ve strand trace disagreeing with consensus
set_def CONTIG_EDITOR.AUTO_DISPLAY_TRACES_CONF "+ - +d -d"
set_def CONTIG_EDITOR.AUTO_DIFF_TRACES 0
set_def CONTIG_EDITOR.READ_PAIR_TRACES 0
set_def CONTIG_EDITOR.REMOVE_HOLES 0
set_def CHECK_ASSEMBLY.COLOUR green4
set_def READPAIR.COLOUR #0000FF
set_def FINDREP.COLOUR #FF3030
set_def FIJ.COLOUR #000000
set_def FINDOLIGO.COLOUR purple
#-----------------------------------------------------------------------------
# Window sizes
#-----------------------------------------------------------------------------
set_def CONTIG_SEL.PLOT_WIDTH 500
set_def CONTIG_SEL.PLOT_HEIGHT 50
set_def R_ENZ.PLOT_WIDTH 700
set_def R_ENZ.PLOT_HEIGHT 500
set_def R_ENZ.NAME_WIDTH 100
set_def R_ENZ.RULER_HEIGHT 30
set_def STOP_CODON.PLOT_WIDTH 700
set_def STOP_CODON.PLOT_HEIGHT 70
set_def STOP_CODON.NAME_WIDTH 100
set_def STOP_CODON.RULER_HEIGHT 30
set_def TEMPLATE.PLOT_WIDTH 700
set_def TEMPLATE.PLOT_HEIGHT 400
set_def TEMPLATE.RULER_HEIGHT 35
#-----------------------------------------------------------------------------
# Window names
#-----------------------------------------------------------------------------
set_def CONTIG_SEL.WIN .contig_sel
set_def CONTIG_CSV.WIN .cs_v
set_def CONTIG_CSP.WIN .cs_plot
set_def TEXTBOXGEN.WIN .text_box
set_def QUALITY_PLOT.WIN .quality_plot
set_def FIJ.WIN .fij
set_def AUTO_ASSEMBLE.1.WIN .shotgun
set_def AUTO_ASSEMBLE.2.WIN .screen
set_def AUTO_ASSEMBLE.3.WIN .one_contig
set_def AUTO_ASSEMBLE.4.WIN .new_contig
set_def AUTO_ASSEMBLE.5.WIN .assemble_into_single_stranded_regions
set_def AUTO_ASSEMBLE.6.WIN .ignore_previous_data
set_def FINDREP.WIN .find_repeats
set_def BREAK_CONTIG.WIN .break_contig
set_def LONGGELS.WIN .long_gels
set_def TTERM.WIN .compressions_stops
set_def TEMPLATE.WIN .show_templates
set_def TEMPLATE.TEMPLATE.WIN .items
set_def TEMPLATE.CURSOR1 .cursor_t
set_def TEMPLATE.CURSOR2 .cursor_l
set_def READING_LIST.WIN .reading_list
set_def SHOWREL.WIN .show_relationships
set_def DIS_READINGS.WIN .disassemble_readings
set_def DIS_CONTIGS.WIN .disassemble_contigs
set_def EXTRACT.WIN .extract_readings
set_def PREASS.WIN .preassemble
set_def CONTIG_EDITOR.WIN .cedit
set_def TRACE_DISPLAY.WIN .traces
set_def TAG_EDITOR.WIN .tag
set_def DOCTOR_DATABASE.WIN .doctor
set_def COMPLEMENT_CONTIG.WIN .complement_contig
set_def QUALITY_CLIP.WIN .quality_clip
set_def N_CLIP.WIN .nbase_clip
set_def Q_CLIP_ENDS.WIN .qclip_ends
set_def DIFFERENCE_CLIP.WIN .difference_clip
set_def DOUBLE_STRAND.WIN .double_strand
set_def SELECT_TAGS.WIN .select_tags
set_def CONSENSUS.1.WIN .normal
set_def CONSENSUS.2.WIN .extended
set_def CONSENSUS.3.WIN .unfinished
set_def CONSENSUS.4.WIN .quality
set_def LIST_LISTS.WIN .list_lists
set_def DELETE_LIST.WIN .delete_list
set_def LOAD_LIST.WIN .load_list
set_def SAVE_LIST.WIN .save_list
set_def EDIT_LIST.WIN .edit_list
set_def CREATE_LIST.WIN .create_list
set_def PRINT_LIST.WIN .print_list
set_def COPY_LIST.WIN .copy_list
set_def PAIR_READINGS.WIN .pair_readings
set_def NUMLIST_TO_NAME.WIN .num_to_name
set_def CONTIGS_TO_LIST.WIN .contigs_to_readings
set_def SUGGEST_PRIMERS.WIN .suggest_primers
set_def CALC_QUALITY.WIN .calc_quality
set_def READPAIR.WIN .read_pairs
set_def MINIMAL_COVERAGE.WIN .minimal_coverage
set_def UNATTACHED_READINGS.WIN .unattached_readings
set_def CONFIGURE.WIN .configure
set_def CONFMAXSEQ.WIN .confmaxseq
set_def COPY_DATABASE.WIN .copy_database
set_def ENTER_TAGS.WIN .enter_tags
set_def R_ENZ.WIN .restriction_enzymes
set_def R_ENZ_LIST.WIN .list_of_restriction_enzymes
set_def R_ENZ_LIST2.WIN .list_of_restriction_enzymes2
set_def STOP_CODON_DIAL.WIN .stop_codons_dialog
set_def STOP_CODON.WIN .stop_codons
set_def DIRECT_ASSEMBLY.WIN .direct_assembly
set_def CHECK_ASSEMBLY.WIN .check_assembly
set_def DUMP_CONTIG.WIN .dump_contig
set_def SAVE_CON_TRACE.WIN .consensus_trace
set_def FINDOLIGO.WIN .find_oligos
set_def FIND_PROBES.WIN .suggest_probes
set_def LIST_CONFIDENCE.WIN .list_confidence
set_def SHUFFLE_PADS.WIN .shuffle_pads
set_def REMOVE_PAD_COLUMNS.WIN .remove_pad_columns
#-----------------------------------------------------------------------------
# Tag type lists.
# Combine out of:
# * for all
# -COMM except for COMMent tags
# COMM or +COMM with COMMent tags
# Eg "* -SVEC -CVEC" for all tags except the vector ones.
#-----------------------------------------------------------------------------
set_def CONTIG_SEL.TAGS {}
set_def TEMPLATE.TAGS {*}
set_def CONTIG_EDITOR.TAGS {* -BIOL}
set_def CONSENSUS.TAGS {REPT ALUS MASK}
set_def AUTO_ASSEMBLE.TAGS {REPT ALUS MASK}
set_def FIJ.TAGS {REPT ALUS MASK}
set_def FINDREP.TAGS {REPT ALUS MASK}
set_def FINDOLIGO.TAGS {OLIG}
#-----------------------------------------------------------------------------
# Useful common questions - asked by many options
#-----------------------------------------------------------------------------
set_defx defs_list NAME "List or file name"
set_defx defs_list VALUE ""
set_defx defs_fofn NAME "File of filenames"
set_defx defs_fofn VALUE ""
set_defx defs_forn NAME "File of reading names"
set_defx defs_forn VALUE ""
set_defx defs_fof NAME "File for names of failures"
set_defx defs_forn VALUE ""
set_defx defs_rn NAME "Gel reading name"
set_defx defs_rn VALUE ""
set_defx defs_usefofn NAME "Use file of filenames"
set_defx defs_usefofn VALUE 1
set_defx defs_minval NAME "Minimum value"
set_defx defs_minval MIN 0
set_defx defs_minval MAX 0
set_defx defs_minval VALUE 0
set_defx defs_seltask NAME "Select task"
set_defx defs_seltask VALUE 1
set_defx defs_procmode NAME "Select processing mode"
set_defx defs_procmode VALUE 1
set_defx defs_procmode BUTTON.1 "Process all contigs"
set_defx defs_procmode BUTTON.2 "Select contig"
set_defx defs_maxmis NAME "Maximum percent mismatch"
set_defx defs_maxmis MIN 0.00
set_defx defs_maxmis MAX 100.0
set_defx defs_maxmis RES 0.01
#FIXME - remove these eventually!
set_defx defs_l_or_f NAME "Save values in"
set_defx defs_l_or_f VALUE 2
set_defx defs_l_or_f BUTTON.1 neither
set_defx defs_l_or_f BUTTON.2 list
set_defx defs_l_or_f BUTTON.3 file
set_defx defs_hidden NAME "Use hidden data"
set_defx defs_hidden VALUE 0
set_defx defs_f_or_l_in WHICH.NAME "Input from"
set_defx defs_f_or_l_in WHICH.BUTTONS {list file}
set_defx defs_f_or_l_in WHICH.VALUE 1
set_defx defs_f_or_l_in NAME.NAME "List or file name"
set_defx defs_f_or_l_in NAME.BROWSE "Browse"
set_defx defs_f_or_l_in NAME.VALUE "readings"
set_defx defs_c_in WHICH.NAME "Input contigs from"
set_defx defs_c_in WHICH.BUTTONS {list file {{all contigs}} single}
set_defx defs_c_in WHICH.VALUE 3
set_defx defs_c_in NAME.NAME "List or file name"
set_defx defs_c_in NAME.BROWSE "Browse"
set_defx defs_c_in NAME.VALUE ""
set_defx defs_f_or_l_out WHICH.NAME "Output to"
set_defx defs_f_or_l_out WHICH.BUTTONS {list file}
set_defx defs_f_or_l_out WHICH.VALUE 1
set_defx defs_f_or_l_out NAME.NAME "List or file name"
set_defx defs_f_or_l_out NAME.BROWSE "Browse"
set_defx defs_f_or_l_out NAME.VALUE "r_out"
set_defx defs_format NAME "Select format"
set_defx defs_format VALUE 2
set_defx defs_format BUTTON.1 fastq
set_defx defs_format BUTTON.2 fasta
set_defx defs_format BUTTON.3 experiment
# Globally used questions
set_def GLOBAL.READING $defs_rn
#-----------------------------------------------------------------------------
# The Primer3 defaults
#-----------------------------------------------------------------------------
set_def PRIMER.min_tm 50
set_def PRIMER.opt_tm 55
set_def PRIMER.max_tm 60
set_def PRIMER.min_gc 20
set_def PRIMER.opt_gc 50
set_def PRIMER.max_gc 80
set_def PRIMER.min_len 17
set_def PRIMER.opt_len 20
set_def PRIMER.max_len 23
set_def PRIMER.max_poly_x 5
set_def PRIMER.self_any 5
set_def PRIMER.self_end 3
set_def PRIMER.gc_clamp 1
set_def PRIMER.max_end_stability 9
set_def PRIMER.dna_conc 50; # nanomolar
set_def PRIMER.salt_conc 50; # millimolar
set_def PRIMER.mg_conc 1.5; # millimolar
set_def PRIMER.dntp_conc 0.8; # millimolar
#-----------------------------------------------------------------------------
# Our options
#-----------------------------------------------------------------------------
# "Screen against vector" and "screen against enzymes" share a few
# questions:
#contig identifer widget
set_def CONTIGID.NAME "Contig identifier"
set_def CONTIGID.VALUE ""
#readings list
set_def READING_LIST.INFILE $defs_f_or_l_in
set_def READING_LIST.INFILE.WHICH.NAME "Input readings from"
set_def READING_LIST.INFILE.WHICH.BUTTONS {list file }
set_def READING_LIST.INFILE.WHICH.VALUE 1
#Map Reads
set_def MAP_READS.INFILE $defs_c_in
set_def MAP_READS.INFILE.WHICH.NAME "Contig(s) to compare against"
set_def MAP_READS.INDEX_SEQUENCE_NAMES 1
set_def MAP_READS.OUTPUT_FN "failures.seq"
#find read pair
set_def READPAIR.INFILE $defs_c_in
set_def READPAIR.INFILE.WHICH.BUTTONS {list file {{all contigs}}}
set_def READPAIR.LINEWIDTH 2
set_def READPAIR.MODE 1
set_def READPAIR.END_SIZE 1000
set_def READPAIR.MIN_MAP_QUAL 10
set_def READPAIR.MIN_FREQ 0
#find repeats
set_def FINDREP.INFILE $defs_c_in
set_def FINDREP.MINREP.NAME "Minimum Repeat"
set_def FINDREP.MINREP.MIN 8
set_def FINDREP.MINREP.MAX 1000
set_def FINDREP.MINREP.VALUE 25
set_def FINDREP.SELTASK $defs_seltask
set_def FINDREP.SELTASK.BUTTON.1 "Find direct repeats"
set_def FINDREP.SELTASK.BUTTON.2 "Find inverted repeats"
set_def FINDREP.SELTASK.BUTTON.3 "Find both"
set_def FINDREP.SELMODE.NAME "Select Masking"
set_def FINDREP.SELMODE.VALUE 2
set_def FINDREP.SELMODE.BUTTON.1 "Mask active tags"
set_def FINDREP.SELMODE.BUTTON.2 "No masking"
set_def FINDREP.PROCMODE $defs_procmode
set_def FINDREP.ADDTAGS.NAME "Add tags to database"
set_def FINDREP.ADDTAGS.VALUE 1
set_def FINDREP.SAVETAGS.SAVENAME.NAME "File name"
set_def FINDREP.SAVETAGS.SAVENAME.VALUE ""
set_def FINDREP.SAVETAGS.SAVEYN.NAME "Save tags to file"
set_def FINDREP.SAVETAGS.SAVEYN.VALUE 0
set_def FINDREP.LINEWIDTH 2
#Show relationships
set_def SHOWREL.PROCMODE $defs_procmode
set_def SHOWREL.INFILE $defs_c_in
set_def SHOWREL.INFILE.NAME.VALUE "contigs"
set_def SHOWREL.POS.NAME "Show readings in positional order"
set_def SHOWREL.POS.VALUE 1
#Hidden data selection sub-dialogue
set_def HIDDEN.MODE.NAME "Use confidence values"
set_def HIDDEN.MODE.VALUE 1
set_def HIDDEN.UNC.LABEL "Uncalled base parameters"
set_def HIDDEN.UNC.WINSIZE.NAME "Window size"
set_def HIDDEN.UNC.WINSIZE.MIN 1
set_def HIDDEN.UNC.WINSIZE.MAX 200
set_def HIDDEN.UNC.WINSIZE.VALUE 50
set_def HIDDEN.UNC.MAXDASH.NAME "Max number of uncalled bases in window"
set_def HIDDEN.UNC.MAXDASH.MIN 1
set_def HIDDEN.UNC.MAXDASH.MAX 20
set_def HIDDEN.UNC.MAXDASH.VALUE 5
set_def HIDDEN.CONF.LABEL "Confidence value parameters"
set_def HIDDEN.CONF.WINSIZE.NAME "Window size"
set_def HIDDEN.CONF.WINSIZE.MIN 1
set_def HIDDEN.CONF.WINSIZE.MAX 200
set_def HIDDEN.CONF.WINSIZE.VALUE 50
set_def HIDDEN.CONF.MINCONF.NAME "Minimum average confidence"
set_def HIDDEN.CONF.MINCONF.MIN 0
set_def HIDDEN.CONF.MINCONF.MAX 100
set_def HIDDEN.CONF.MINCONF.VALUE 15
#Find internal joins
set_def FIJ.INFILE1 $defs_c_in
set_def FIJ.INFILE1.WHICH.NAME "Compare contigs from"
set_def FIJ.INFILE2 $defs_c_in
set_def FIJ.INFILE2.WHICH.NAME "With contigs from"
set_def FIJ.MINOVERLAP.NAME "Minimum overlap"
set_def FIJ.MINOVERLAP.VALUE 20
set_def FIJ.MAXOVERLAP.NAME "Maximum overlap"
set_def FIJ.MAXOVERLAP.VALUE 10000000
set_def FIJ.MINMATCH.NAME "Minimum initial match length"
set_def FIJ.MINMATCH.VALUE 20
set_def FIJ.MINDEPTH.NAME "Minimum combined depth"
set_def FIJ.MINDEPTH.VALUE 0
set_def FIJ.MAXDEPTH.NAME "Maximum combined depth"
set_def FIJ.MAXDEPTH.VALUE 0
set_def FIJ.MAXDIAG.NAME "Diagonal threshold"
set_def FIJ.MAXDIAG.MIN 1.0e-40
set_def FIJ.MAXDIAG.MAX 1.0e-4
set_def FIJ.MAXDIAG.VALUE 1.0e-8
set_def FIJ.BANDSIZE.NAME "Alignment band size (percent)"
set_def FIJ.BANDSIZE.MIN 1
set_def FIJ.BANDSIZE.MAX 100
set_def FIJ.BANDSIZE.VALUE 5
set_def FIJ.MAXMIS $defs_maxmis
set_def FIJ.MAXMIS.VALUE 30.00
set_def FIJ.WORDLENGTH.NAME "Word length"
set_def FIJ.WORDLENGTH.BUTTON.1 12
set_def FIJ.WORDLENGTH.BUTTON.2 8
set_def FIJ.WORDLENGTH.BUTTON.3 4
set_def FIJ.WORDLENGTH.VALUE 1
set_def FIJ.USEFILTERWORDS.NAME "Filter repeat words"
set_def FIJ.USEFILTERWORDS.VALUE 0
set_def FIJ.FILTERWORDS.NAME "Maximum word over-representation"
set_def FIJ.FILTERWORDS.VALUE 5
set_def FIJ.UNIQUE_ENDS 0
set_def FIJ.HIDDEN.WINSIZE.NAME "Window length"
set_def FIJ.HIDDEN.MAXDASH.NAME "Window length"
set_def FIJ.HIDDEN $defs_hidden
set_def FIJ.MINCONF.NAME "Minimum confidence"
set_def FIJ.MINCONF.MIN 0
set_def FIJ.MINCONF.MAX 100
set_def FIJ.MINCONF.VALUE 8
set_def FIJ.SELMODE.NAME "Select Mode"
set_def FIJ.SELMODE.VALUE 1
set_def FIJ.SELMODE.BUTTON.1 "Use standard consensus"
set_def FIJ.SELMODE.BUTTON.2 "Mark active tags"
set_def FIJ.SELMODE.BUTTON.3 "Mask active tags"
set_def FIJ.LINEWIDTH 2
set_def FIJ.MAX_ALIGNMENT 4000
# Alignmode 1 = quickest, 2 = quick, 3 = sensitive (slower)
set_def FIJ.ALIGN_MODE 2
# Alignment location 1 = end/end, 2 = containments, 3 = both
set_def FIJ.ALIGN_LOCATION 3
# Only applies for the quick algorithm
set_def FIJ.USEBAND.NAME "Use banded alignment"
set_def FIJ.USEBAND.VALUE 1
# FIJ readpair settings
set_def FIJ.READPAIR.MODE.NAME "Read-pair filter"
set_def FIJ.READPAIR.MODE.BUTTON1 "All vs All"
set_def FIJ.READPAIR.MODE.BUTTON2 "End vs All"
set_def FIJ.READPAIR.MODE.BUTTON3 "End vs End"
set_def FIJ.READPAIR.MODE.BUTTON4 "Off"
set_def FIJ.READPAIR.DIALOG.BUTTON.NAME "Edit read pair filter parameters"
set_def FIJ.READPAIR.DIALOG.NAME "Read pair filter parameters"
set_def FIJ.READPAIR.END_SIZE.NAME "Size of contig 'ends'"
set_def FIJ.READPAIR.END_SIZE.VALUE 1000
set_def FIJ.READPAIR.MIN_MAP_QUAL.NAME "Minimum mapping quality"
set_def FIJ.READPAIR.MIN_MAP_QUAL.VALUE 10
set_def FIJ.READPAIR.MIN_MAP_QUAL.MIN 0
set_def FIJ.READPAIR.MIN_MAP_QUAL.MAX 100
set_def FIJ.READPAIR.MIN_FREQ.NAME "Minimum spanning pair count"
set_def FIJ.READPAIR.MIN_FREQ.VALUE 1
set_def FIJ.READPAIR.MIN_FREQ.MIN 1
set_def FIJ.READPAIR.MIN_FREQ.MAX 100
set_def FIJ.READPAIR.MIN_PERC.NAME "Minimum spanning pair percentage"
set_def FIJ.READPAIR.MIN_PERC.VALUE 70
set_def FIJ.READPAIR.MIN_PERC.MIN 1
set_def FIJ.READPAIR.MIN_PERC.MAX 100
#Auto assemble
set_def AUTO_ASSEMBLE.USE_EXP_ARCHIVES 0
set_def AUTO_ASSEMBLE.MASKING.NAME "Apply masking"
set_def AUTO_ASSEMBLE.MASKING.VALUE 0
set_def AUTO_ASSEMBLE.DISPMODE.NAME "Select display mode"
set_def AUTO_ASSEMBLE.DISPMODE.VALUE 1
set_def AUTO_ASSEMBLE.DISPMODE.BUTTON.1 "Hide all alignments"
set_def AUTO_ASSEMBLE.DISPMODE.BUTTON.2 "Show passed alignments"
set_def AUTO_ASSEMBLE.DISPMODE.BUTTON.3 "Show all alignments"
set_def AUTO_ASSEMBLE.DISPMODE.BUTTON.4 "Show only failed alignments"
set_def AUTO_ASSEMBLE.MINMATCH.NAME "Minimum initial match"
set_def AUTO_ASSEMBLE.MINMATCH.MIN 14
set_def AUTO_ASSEMBLE.MINMATCH.MAX 300
set_def AUTO_ASSEMBLE.MINMATCH.VALUE 20
set_def AUTO_ASSEMBLE.MAXPADS.NAME "Maximum pads per read"
set_def AUTO_ASSEMBLE.MAXPADS.MIN 0
set_def AUTO_ASSEMBLE.MAXPADS.MAX 100
set_def AUTO_ASSEMBLE.MAXPADS.VALUE 25
set_def AUTO_ASSEMBLE.MISMATCH.NAME "Maximum percent mismatch"
set_def AUTO_ASSEMBLE.MISMATCH.MIN 0.00
set_def AUTO_ASSEMBLE.MISMATCH.MAX 100.0
set_def AUTO_ASSEMBLE.MISMATCH.VALUE 5.00
set_def AUTO_ASSEMBLE.MISMATCH.DIGITS 0
set_def AUTO_ASSEMBLE.MISMATCH.RES 0.01
set_def AUTO_ASSEMBLE.SAVEALIGN $defs_f_or_l_out
set_def AUTO_ASSEMBLE.SAVEALIGN.WHICH.NAME "Save alignment scores in "
set_def AUTO_ASSEMBLE.INFILE $defs_f_or_l_in
set_def AUTO_ASSEMBLE.INFILE.WHICH.NAME "Input reading filenames from"
if ![catch {set tmptmp $env(BIOLIMS_CONF)}] {
set_def AUTO_ASSEMBLE.INFILE.WHICH.BUTTONS {list file selection BioLIMS}
} else {
set_def AUTO_ASSEMBLE.INFILE.WHICH.BUTTONS {list file selection}
}
set_def AUTO_ASSEMBLE.FAILS $defs_f_or_l_out
set_def AUTO_ASSEMBLE.FAILS.WHICH.NAME "Save failures to"
set_def AUTO_ASSEMBLE.FOF.MODE $defs_l_or_f
set_def AUTO_ASSEMBLE.FOF.MODE.NAME "Save failures in"
set_def AUTO_ASSEMBLE.FOF.MODE.VALUE 1
set_def AUTO_ASSEMBLE.FOF.FILE.NAME "Name for failures"
set_def AUTO_ASSEMBLE.FOF.FILE.VALUE "fails"
set_def AUTO_ASSEMBLE.HIDDEN $defs_hidden
set_def AUTO_ASSEMBLE.PERMITJOINS.NAME "Permit joins"
set_def AUTO_ASSEMBLE.PERMITJOINS.VALUE 1
set_def AUTO_ASSEMBLE.ALIGNMENT_TOOL 3
set_def AUTO_ASSEMBLE.FAILURE.NAME "Select alignment failure mode"
set_def AUTO_ASSEMBLE.FAILURE.BUTTON.1 "Reject failures"
set_def AUTO_ASSEMBLE.FAILURE.BUTTON.2 "Enter all readings"
set_def AUTO_ASSEMBLE.FAILURE.VALUE 2
set_def AUTO_ASSEMBLE.SCORE.NAME "Save alignment scores in a file"
set_def AUTO_ASSEMBLE.SCORE.VALUE 1
set_def AUTO_ASSEMBLE.SCORE.FILE.NAME "File for names and scores"
set_def AUTO_ASSEMBLE.SCORE.FILE.VALUE scores
set_def AUTO_ASSEMBLE.POORDATA.NAME "Use poor data"
set_def AUTO_ASSEMBLE.POORDATA.VALUE 1
#break contig
set_def BREAK_CONTIG.REMOVE_HOLES 1
#disassemble readings
set_def DIS_READINGS.INFILE $defs_f_or_l_in
set_def DIS_READINGS.INFILE.WHICH.NAME "Input reading names from"
set_def DIS_READINGS.INFILE.WHICH.BUTTONS {list file single}
set_def DIS_READINGS.SELTASK $defs_seltask
set_def DIS_READINGS.SELTASK.BUTTON.1 "Remove readings from database"
set_def DIS_READINGS.SELTASK.BUTTON.2 "Split into single-read contigs"
set_def DIS_READINGS.SELTASK.BUTTON.3 "Move readings to new contigs"
set_def DIS_READINGS.SELTASK.VALUE 3
set_def DIS_READINGS.BREAK_CONTIG 1
set_def DIS_READINGS.DUPCONSTAGS.VALUE 1
#suggest long gels
set_def LONGGELS.GLEN.NAME "Estimated long reading length"
set_def LONGGELS.GLEN.VALUE 700
set_def LONGGELS.INFILE $defs_c_in
#suggest long gels
set_def TTERM.TLEN.NAME "Estimated reading length"
set_def TTERM.TLEN.VALUE 200
set_def TTERM.INFILE $defs_c_in
#extract readings
set_def EXTRACT.INFILE $defs_f_or_l_in
set_def EXTRACT.INFILE.WHICH.NAME "Input reading names from"
set_def EXTRACT.INFILE.WHICH.BUTTONS {list file single}
set_def EXTRACT.DEST.NAME "Destination directory"
set_def EXTRACT.DEST.VALUE "extracts"
set_def EXTRACT.FORMAT.NAME {Output format}
set_def EXTRACT.FORMAT.VALUE 1
set_def EXTRACT.FORMAT.B1 Normal
#set_def EXTRACT.FORMAT.B2 Pre-assemble
set_def EXTRACT.FORMAT.B2 {Directed assembly, anchor left}
set_def EXTRACT.FORMAT.B3 {Directed assembly, anchor start}
#preassemble
#set_def PREASS.USEFOFN.FORN $defs_forn
#set_def PREASS.USEFOFN.FORN.VALUE "files3"
set_def PREASS.INPUT.NAME "Input reading filenames from"
#quality clip
set_def QUALITY_CLIP.MINQUAL.NAME "Minimum average quality at ends"
set_def QUALITY_CLIP.MINQUAL.MIN 0
set_def QUALITY_CLIP.MINQUAL.MAX 50
set_def QUALITY_CLIP.MINQUAL.VALUE 15
set_def QUALITY_CLIP.INFILE $defs_c_in
#N-base clip
set_def N_CLIP.INFILE $defs_c_in
#Quality clip ends
set_def Q_CLIP_ENDS.INFILE $defs_c_in
#difference clip
set_def DIFFERENCE_CLIP.INFILE $defs_c_in
set_def DIFFERENCE_CLIP.TAG.TITLE "Add DIFF tags"
set_def DIFFERENCE_CLIP.TAG.DEFAULT 0
#double stranding
set_def DOUBLE_STRAND.MAXMIS.NAME "Maximum number of mismatches"
set_def DOUBLE_STRAND.MAXMIS.VALUE 8
set_def DOUBLE_STRAND.MAXPERC.NAME "Maximum percentage of mismatches"
set_def DOUBLE_STRAND.MAXPERC.MIN 0
set_def DOUBLE_STRAND.MAXPERC.MAX 100
set_def DOUBLE_STRAND.MAXPERC.VALUE 8
set_def DOUBLE_STRAND.INFILE $defs_c_in
#list code
set_def DELETE_LIST.LISTNAME "list name"
set_def LOAD_LIST.FILENAME "file name"
set_def LOAD_LIST.LISTNAME "list name"
set_def SAVE_LIST.FILENAME "file name"
set_def SAVE_LIST.LISTNAME "list name"
set_def EDIT_LIST.LISTNAME "list name"
set_def CREATE_LIST.LISTNAME "list name"
set_def PRINT_LIST.LISTNAME "list name"
set_def COPY_LIST.FROMLIST "from list name"
set_def COPY_LIST.TOLIST "to list name"
set_def NUMLIST_TO_NAME.FROMLIST "from list name"
set_def NUMLIST_TO_NAME.TOLIST "to list name"
set_def PAIR_READINGS.INFILE $defs_f_or_l_in
set_def PAIR_READINGS.OUTFILE $defs_f_or_l_out
set_def CONTIGS_TO_LIST.INFILE $defs_c_in
set_def CONTIGS_TO_LIST.OUTFILE $defs_f_or_l_out
set_def CONTIGS_TO_LIST.OUTFILE.WHICH.NAME "Save reading names in"
#Export Sequences
set_def EXPORT.INFILE $defs_c_in
set_def EXPORT.FORMAT 2
set_def EXPORT.FIX_MATES 0
set_def EXPORT.DEPAD 1
#calculate consensus
set_def CONSENSUS.INFILE $defs_c_in
set_def CONSENSUS.SELMASK.NAME "Select mode"
set_def CONSENSUS.SELMASK.VALUE 3
set_def CONSENSUS.SELMASK.BUTTON.1 "mask active tags"
set_def CONSENSUS.SELMASK.BUTTON.2 "mark active tags"
set_def CONSENSUS.SELMASK.BUTTON.3 neither
set_def CONSENSUS.NORMAL.FORMAT $defs_format
set_def CONSENSUS.EXTENDED.FORMAT $defs_format
set_def CONSENSUS.UNFINISHED.FORMAT $defs_format
set_def CONSENSUS.QUALITY.FORMAT $defs_format
set_def CONSENSUS.QUALITY.FORMAT.VALUE 1
set_def CONSENSUS.QUALITY.FORMAT.BUTTON.1 staden
set_def CONSENSUS.EXPT.VALUE 1
set_def CONSENSUS.EXPT.BUTTON.1 "all annotations"
set_def CONSENSUS.EXPT.BUTTON.2 "annotations except in hidden"
set_def CONSENSUS.EXPT.BUTTON.3 "no annotations"
set_def CONSENSUS.OUTPUT.NAME "Output file"
set_def CONSENSUS.OUTPUT.VALUE cons
set_def CONSENSUS.NORMAL.STRIP_PADS 1
set_def CONSENSUS.NORMAL.AMBIGUITY_CODES 0
set_def CONSENSUS.EXTENDED.STRIP_PADS 1
set_def CONSENSUS.UNFINISHED.STRIP_PADS 1
set_def CONSENSUS.READ_NOTES 0
set_def CONSENSUS.READ_ANNOTATIONS 1
set_def CONSENSUS.NAME_BY 1
# Suggest primers
set_def SUGGEST_PRIMERS.INFILE $defs_c_in
set_def SUGGEST_PRIMERS.OUTFILE $defs_f_or_l_out
set_def SUGGEST_PRIMERS.OUTFILE.WHICH.NAME "save primers to"
set_def SUGGEST_PRIMERS.SEARCH_START.NAME "search start position"
set_def SUGGEST_PRIMERS.SEARCH_START.MIN 0
set_def SUGGEST_PRIMERS.SEARCH_START.MAX 200
set_def SUGGEST_PRIMERS.SEARCH_START.VALUE 15
set_def SUGGEST_PRIMERS.SEARCH_END.NAME "search end position"
set_def SUGGEST_PRIMERS.SEARCH_END.MIN 0
set_def SUGGEST_PRIMERS.SEARCH_END.MAX 200
set_def SUGGEST_PRIMERS.SEARCH_END.VALUE 80
set_def SUGGEST_PRIMERS.NUM_PRIMERS.NAME "number of primers per match"
set_def SUGGEST_PRIMERS.NUM_PRIMERS.VALUE 1
set_def SUGGEST_PRIMERS.SEARCH_NUM.NAME "primer start number"
set_def SUGGEST_PRIMERS.SEARCH_NUM.VALUE 1
set_def SUGGEST_PRIMERS.PARAMS.TM.NAME "Tm (Celsius)"
set_def SUGGEST_PRIMERS.PARAMS.LEN.NAME "Length (bases)"
set_def SUGGEST_PRIMERS.PARAMS.GC.NAME "G+C Content (%)"
# PCR primers
set_def PCR_PRIMERS.INFILE $defs_c_in
set_def PCR_PRIMERS.INFILE.WHICH.BUTTONS {list file {{all contigs}}}
set_def PCR_PRIMERS.TASK 2
set_def PCR_PRIMERS.SEARCH_START.NAME "Closest to end"
set_def PCR_PRIMERS.SEARCH_START.MIN 0
set_def PCR_PRIMERS.SEARCH_START.MAX 500
set_def PCR_PRIMERS.SEARCH_START.VALUE 15
set_def PCR_PRIMERS.SEARCH_END.NAME "Furthest from end"
set_def PCR_PRIMERS.SEARCH_END.MIN 0
set_def PCR_PRIMERS.SEARCH_END.MAX 500
set_def PCR_PRIMERS.SEARCH_END.VALUE 100
set_def PCR_PRIMERS.PARAMS.TM.NAME "Tm (Celsius)"
set_def PCR_PRIMERS.PARAMS.LEN.NAME "Length (bases)"
set_def PCR_PRIMERS.PARAMS.GC.NAME "G+C Content (%)"
set_def PCR_PRIMERS.TMDIFF.NAME "Maximum Oligo Tm difference"
set_def PCR_PRIMERS.TMDIFF.VALUE 4
# Minimal coverage
set_def MINIMAL_COVERAGE.INFILE $defs_c_in
set_def MINIMAL_COVERAGE.OUTFILE $defs_f_or_l_out
set_def MINIMAL_COVERAGE.OUTFILE.WHICH.NAME "Save output in"
# Unattached readings
set_def UNATTACHED_READINGS.OUTFILE $defs_f_or_l_out
set_def UNATTACHED_READINGS.OUTFILE.WHICH.NAME "Save output in"
# Contig editor select oligos command
# dir 1=fwd 2=rev
set_def SELECT_OLIGOS.DIRECTION 1
set_def SELECT_OLIGOS.SEARCH_AHEAD 40
set_def SELECT_OLIGOS.SEARCH_BACK 40
set_def SELECT_OLIGOS.READ_LENGTH 500
# Contig editor dump contig command
set_def DUMP_CONTIG.NAME "Consensus filename"
set_def DUMP_CONTIG.VALUE cons
set_def DUMP_CONTIG.LINE_LENGTH 50
set_def DUMP_CONTIG.NAME_WIDTH 20
# Contig editor save consensus trace command
set_def SAVE_CON_TRACE.NAME "Trace filename"
set_def SAVE_CON_TRACE.VALUE cons.scf
set_def SAVE_CON_TRACE.STRAND 1
set_def SAVE_CON_TRACE.MATCHING 1
# Configure cutoffs
set_def CONFIGURE.CONSENSUS_NAME "Consensus cutoff"
set_def CONFIGURE.QUALITY_NAME "Quality cutoff"
set_def CONFIGURE.CHEMISTRY_NAME "Use Special chemistry (double strand)"
set_def CONFIGURE.CMODE_NAME "Consensus algorithm"
set_def CONFIGURE.CMODE_BUTTON1 {Base frequencies}
set_def CONFIGURE.CMODE_BUTTON2 {Weighted base frequencies}
set_def CONFIGURE.CMODE_BUTTON3 {Confidence values}
# Set maxseq/maxdb
set_def CONFMAXSEQ.MAXSEQ.NAME {Enter new maxseq (total consensus length)}
set_def CONFMAXSEQ.MAXDB.NAME {Enter new maxdb (readings + contigs)}
# Enter tags
set_def ENTER_TAGS.FILENAME "file name"
# Stop codons
set_def STOP_CODON.STRAND.NAME "Select strand"
set_def STOP_CODON.STRAND.VALUE 1
set_def STOP_CODON.STRAND.BUTTON.1 "Positive strand"
set_def STOP_CODON.STRAND.BUTTON.2 "Complementary strand"
set_def STOP_CODON.STRAND.BUTTON.3 "Both strands"
# Directed assembly
set_def DIRECT_ASSEMBLY.INFILE $defs_f_or_l_in
set_def DIRECT_ASSEMBLY.INFILE.WHICH.NAME "Input readings filenames from"
if ![catch {set tmptmp $env(BIOLIMS_CONF)}] {
set_def DIRECT_ASSEMBLY.INFILE.WHICH.BUTTONS {list file selection BioLIMS}
} else {
set_def DIRECT_ASSEMBLY.INFILE.WHICH.BUTTONS {list file selection}
}
set_def DIRECT_ASSEMBLY.OUTFILE $defs_f_or_l_out
set_def DIRECT_ASSEMBLY.OUTFILE.WHICH.NAME "Save failures to"
set_def DIRECT_ASSEMBLY.MAXMIS $defs_maxmis
set_def DIRECT_ASSEMBLY.MAXMIS.VALUE 10.00
set_def DIRECT_ASSEMBLY.DISPLAY.NAME "Display alignments"
set_def DIRECT_ASSEMBLY.DISPLAY.VALUE 0
set_def DIRECT_ASSEMBLY.IGNORE_VEC.NAME "Ignore svec (SL/SR) clips"
set_def DIRECT_ASSEMBLY.IGNORE_VEC.VALUE 0
set_def DIRECT_ASSEMBLY.FAILURE.NAME "Select alignment failure mode"
set_def DIRECT_ASSEMBLY.FAILURE.BUTTON.1 "Reject failures"
set_def DIRECT_ASSEMBLY.FAILURE.BUTTON.2 "Enter all readings"
set_def DIRECT_ASSEMBLY.FAILURE.VALUE 1
# Check assembly
set_def CHECK_ASSEMBLY.LINEWIDTH 2
set_def CHECK_ASSEMBLY.INFILE $defs_c_in
set_def CHECK_ASSEMBLY.MINLEN.NAME "Minimum length of alignment"
set_def CHECK_ASSEMBLY.MINLEN.VALUE 15
set_def CHECK_ASSEMBLY.MAXPERC.NAME "Maximum percentage of mismatches"
set_def CHECK_ASSEMBLY.MAXPERC.VALUE 50
set_def CHECK_ASSEMBLY.MAXPERC.MIN 0
set_def CHECK_ASSEMBLY.MAXPERC.MAX 100
set_def CHECK_ASSEMBLY.USE_CUTOFF.NAME "Use cutoff data"
set_def CHECK_ASSEMBLY.USE_CUTOFF.VALUE 1
set_def CHECK_ASSEMBLY.HIDDEN.WINSIZE.NAME "Window size for good data scan"
set_def CHECK_ASSEMBLY.HIDDEN.WINSIZE.MIN 1
set_def CHECK_ASSEMBLY.HIDDEN.WINSIZE.MAX 200
set_def CHECK_ASSEMBLY.HIDDEN.WINSIZE.VALUE 50
set_def CHECK_ASSEMBLY.HIDDEN.MAXDASH.NAME "Max number of dashes in scan window"
set_def CHECK_ASSEMBLY.HIDDEN.MAXDASH.MIN 1
set_def CHECK_ASSEMBLY.HIDDEN.MAXDASH.MAX 20
set_def CHECK_ASSEMBLY.HIDDEN.MAXDASH.VALUE 5
set_def CHECK_ASSEMBLY.USED.WINSIZE.NAME "Window size for used data"
set_def CHECK_ASSEMBLY.USED.WINSIZE.MIN 1
set_def CHECK_ASSEMBLY.USED.WINSIZE.MAX 1000
set_def CHECK_ASSEMBLY.USED.WINSIZE.VALUE 50
# Shuffle Pads
set_def SHUFFLE_PADS.INFILE $defs_c_in
set_def SHUFFLE_PADS.BAND_SIZE 15
set_def SHUFFLE_PADS.SOFT_CLIPS 1
set_def SHUFFLE_PADS.MAX_PASS 10
set_def SHUFFLE_PADS.EDITOR_BAND_SIZE 51
set_def SHUFFLE_PADS.EDITOR_SOFT_CLIPS 1
set_def SHUFFLE_PADS.EDITOR_MAX_PASS 10
# Remove Pad Columns
set_def REMOVE_PAD_COLUMNS.INFILE $defs_c_in
set_def REMOVE_PAD_COLUMNS.PERCENT_PAD 100
# Find oligos
set_def FINDOLIGO.INFILE $defs_c_in
set_def FINDOLIGO.MAXMIS $defs_maxmis
set_def FINDOLIGO.MAXMIS.NAME "Minimum percent match"
set_def FINDOLIGO.MAXMIS.VALUE 100.00
set_def FINDOLIGO.SELMODE.NAME "Select sequence to match"
set_def FINDOLIGO.SELMODE.VALUE 2
set_def FINDOLIGO.SELMODE.BUTTON.1 "Use tags"
set_def FINDOLIGO.SELMODE.BUTTON.2 "Enter sequence"
set_def FINDOLIGO.SELMODE.BUTTON.3 "Use FASTA file"
set_def FINDOLIGO.MATCHSEQ.NAME "Sequence"
set_def FINDOLIGO.MATCHSEQ.VALUE ""
set_def FINDOLIGO.MATCHSEQ.FASTA ""
set_def FINDOLIGO.LINEWIDTH 2
set_def FINDOLIGO.MAX_MATCHES 1000
set_def FINDOLIGO.CONSONLY.VALUE 1
set_def FINDOLIGO.CUTOFFS.VALUE 1
# Find probes
set_def FIND_PROBES.MAXPERC.NAME "Maximum percentage match"
set_def FIND_PROBES.MAXPERC.MIN 1
set_def FIND_PROBES.MAXPERC.MAX 100
set_def FIND_PROBES.MAXPERC.VALUE 90
set_def FIND_PROBES.OLIGOSIZE.MIN_DEF 17
set_def FIND_PROBES.OLIGOSIZE.MAX_DEF 20
set_def FIND_PROBES.OLIGOSIZE.MIN 10
set_def FIND_PROBES.OLIGOSIZE.MAX 30
set_def FIND_PROBES.OLIGOSIZE.MIN_NAME "Minimum size of oligo"
set_def FIND_PROBES.OLIGOSIZE.MAX_NAME "Maximum size of oligo"
set_def FIND_PROBES.OLIGOPOS.MIN_DEF 10
set_def FIND_PROBES.OLIGOPOS.MAX_DEF 100
set_def FIND_PROBES.OLIGOPOS.MIN 0
set_def FIND_PROBES.OLIGOPOS.MAX 1000
set_def FIND_PROBES.OLIGOPOS.MIN_NAME "Search from offset into contig"
set_def FIND_PROBES.OLIGOPOS.MAX_NAME "Search to offset into contig"
set_def FIND_PROBES.FROM.NAME "Search from offset into contig"
set_def FIND_PROBES.FROM.MIN 0
set_def FIND_PROBES.FROM.MAX 1000
set_def FIND_PROBES.FROM.VALUE 10
set_def FIND_PROBES.TO.NAME "Search to offset into contig"
set_def FIND_PROBES.TO.MIN 10
set_def FIND_PROBES.TO.MAX 1010
set_def FIND_PROBES.TO.VALUE 100
set_def FIND_PROBES.INFILE $defs_c_in
set_def FIND_PROBES.VECTOR.NAME "File of vector files (optional)"
set_def FIND_PROBES.VECTOR.VALUE ""
set_def FIND_PROBES.TAG.NAME "Create tags"
set_def FIND_PROBES.TAG.VALUE 1
set_def FIND_PROBES.OUTFILE.NAME "Output filename (optional)"
set_def FIND_PROBES.OUTFILE.VALUE ""
set_def FIND_PROBES.TAG_TYPE OLIG
set_def COPY_DATABASE.COLLECT 1
#Template display
#ruler details
set_defx defs_ruler WIN .ruler
set_defx defs_ruler COLOUR "black"
set_defx defs_ruler LINE_WIDTH 2
set_defx defs_ruler PLOT_HEIGHT 50
set_defx defs_ruler OFFSET 15
set_defx defs_ruler TEXT_OFFSET 17
set_defx defs_ruler TICK_HEIGHT 10
set_defx defs_ruler TICK_WIDTH 0
set_defx defs_ruler TICK_COLOUR "black"
set_defx defs_ruler NUM_TICKS 4
set_defx defs_ruler TAG_OFFSET 10
set_defx defs_ruler TAG_WIDTH 4
#vertical ruler details
set_defx defs_ruler_v WIN .ruler_v
set_defx defs_ruler_v COLOUR "black"
set_defx defs_ruler_v LINE_WIDTH 2
set_defx defs_ruler_v PLOT_WIDTH 50
set_defx defs_ruler_v OFFSET 15
set_defx defs_ruler_v TEXT_OFFSET 17
set_defx defs_ruler_v TICK_HEIGHT 10
set_defx defs_ruler_v TICK_WIDTH 0
set_defx defs_ruler_v TICK_COLOUR "black"
set_defx defs_ruler_v NUM_TICKS 4
set_defx defs_ruler_v TAG_OFFSET 10
set_defx defs_ruler_v TAG_WIDTH 4
set_def RULER $defs_ruler
set_defx defs_quality WIN .quality
set_defx defs_quality PLOT_WIDTH 700
set_defx defs_quality PLOT_HEIGHT 50
set_defx defs_quality BOTH_COLOUR "SlateGrey"
set_defx defs_quality PLUS_COLOUR "blue"
set_defx defs_quality MINUS_COLOUR "green3"
set_defx defs_quality BAD_COLOUR "red"
set_defx defs_quality DISAGREE_COLOUR "black"
set_defx defs_quality AT_STARTUP 0
set_defx defs_renz WIN .r_enz
set_defx defs_renz PLOT_WIDTH 700
set_defx defs_renz PLOT_HEIGHT 50
set_defx defs_renz TICK_HEIGHT 14
set_defx defs_renz TICK_WIDTH 3
set_defx defs_renz TICK_COLOUR "black"
#standalone quality display
set_def QUALITY $defs_quality
set_def QUALITY.RULER $defs_ruler
set_def QUALITY.BORDERWIDTH 2
set_def QUALITY.CURSOR .cursor_t
set_def QUALITY.CURSOR_COLOUR "black"
set_def QUALITY.CURSOR_WIDTH 0
#template display
set_def TEMPLATE.INFILE $defs_c_in
set_def TEMPLATE.INFILE.NAME.VALUE "contigs"
set_def TEMPLATE.CONFIG.TEMPLATE 1
set_def TEMPLATE.CONFIG.READING 1
set_def TEMPLATE.CONFIG.MULTI_TEMPLATE 0
set_def TEMPLATE.CONFIG.READ_PAIR 0
set_def TEMPLATE.CONFIG.SPAN_READ_PAIR 0
set_def TEMPLATE.CONFIG.CALC_CONTIG_POS 0
set_def TEMPLATE.RULER $defs_ruler
set_def TEMPLATE.RULER.TICK_HEIGHT 6
set_def TEMPLATE.RULER.TAG_OFFSET 7
set_def TEMPLATE.RULER.TEXT_OFFSET 14
set_def TEMPLATE.RULER.OFFSET 20
set_def TEMPLATE.QUALITY $defs_quality
set_def TEMPLATE.RENZ $defs_renz
set_def TEMPLATE.BORDERWIDTH 2
set_def TEMPLATE.CURSOR_WIDTH 0
set_def TEMPLATE.CURSOR_COLOUR "black"
set_def TEMPLATE.PLOT_WIDTH 700
set_def TEMPLATE.PLOT_HEIGHT 400
set_def TEMPLATE.LINE_WIDTH 2
set_def TEMPLATE.LINE_BOLD 4
set_def TEMPLATE.BACKGROUND black
set_def TEMPLATE.SPANNING_COL0 #ffa500
set_def TEMPLATE.SPANNING_COL1 #ffff00
set_def TEMPLATE.SPANNING_COL2 #e02060
set_def TEMPLATE.SPANNING_COL3 #ff00c8
set_def TEMPLATE.SPANNING_COL4 #00dd00
set_def TEMPLATE.SPANNING_COL5 #007000
set_def TEMPLATE.SPANNING_COL6 #00b4ce
set_def TEMPLATE.SPANNING_COL7 #ffdc8c
set_def TEMPLATE.SPANNING_COL8 #ff70a3
set_def TEMPLATE.SPANNING_COL9 #ff4100
set_def TEMPLATE.SINGLE_COL blue
set_def TEMPLATE.INCONSISTENT_COL red
set_def TEMPLATE.FWD_COL green4
set_def TEMPLATE.REV_COL magenta
set_def TEMPLATE.XSCALE 20.0
set_def TEMPLATE.YSCALE 100
set_def TEMPLATE.YOFFSET 50
set_def TEMPLATE.YSPREAD 10
set_def TEMPLATE.MINYSIZE 1024
set_def TEMPLATE.AUTO_UPDATE 1
set_def TEMPLATE.WIDTH 900
set_def TEMPLATE.HEIGHT 600
set_def TEMPLATE.TRACKS {template depth}
#standalone restriction enzyme display
set_def R_ENZ $defs_renz
set_def R_ENZ.RULER $defs_ruler
set_def R_ENZ.BORDERWIDTH 2
set_def R_ENZ.CURSOR .cursor_t
set_def R_ENZ.CURSOR_COLOUR "black"
set_def R_ENZ.CURSOR_WIDTH 0
set_def R_ENZ.NAMES.PLOT_WIDTH 100
set_def R_ENZ.PLOT_HEIGHT 500
set_def R_ENZ.CUTS_COLOUR "black"
set_def R_ENZ.SELECT_COLOUR "pink"
set_def R_ENZ.TEXT_COLOUR "black"
set_def CODON.WIN .codon
set_def CODON.RULER $defs_ruler
set_def CODON.PLOT_WIDTH 700
set_def CODON.PLOT_HEIGHT 70
set_def CODON.TICK_HEIGHT 14
set_def CODON.TICK_WIDTH 3
set_def CODON.TICK_COLOUR "black"
set_def CODON.BORDERWIDTH 2
set_def CODON.CURSOR .cursor_t
set_def CODON.CURSOR_WIDTH 0
set_def CODON.CURSOR_COLOUR "black"
set_def CODON.NAMES.PLOT_WIDTH 100
set_def CODON.TEXT_COLOUR "black"
#contig selector
set_def CONTIG_SEL.DISPLAY_AT_STARTUP 1
set_def CONTIG_SEL.WIN .contig_sel
set_def CONTIG_SEL.BORDERWIDTH 2
set_def CONTIG_SEL.LINE_WIDTH 2
set_def CONTIG_SEL.LINE_BOLD 4
set_def CONTIG_SEL.COLOUR "black"
set_def CONTIG_SEL.CURSOR_WIDTH 0
set_def CONTIG_SEL.CURSOR_COLOUR "black"
set_def CONTIG_SEL.CURSOR1_X .cursor_tx
set_def CONTIG_SEL.CURSOR2_X .cursor_lx
set_def CONTIG_SEL.CURSOR1_Y .cursor_ty
set_def CONTIG_SEL.CURSOR2_Y .cursor_ly
set_def CONTIG_SEL.TICK_HEIGHT 14
set_def CONTIG_SEL.TICK_WIDTH 2
set_def CONTIG_SEL.TICK_COLOUR "black"
set_def CONTIG_SEL.TAG_OFFSET 15
set_def CONTIG_SEL.TAG_WIDTH 3
set_def CONTIG_SEL.EDITOR_POS_1 0
# List confidence
set_def LIST_CONFIDENCE.INFILE $defs_c_in
set_def LIST_CONFIDENCE.SUMMARY.NAME "Summary only"
set_def LIST_CONFIDENCE.SUMMARY.VALUE 1
# Note selector
set_def NOTE_SELECTOR.WIN .note_selector
set_def NOTE_EDITOR.WIN .note_
#set_def NOTE_EDITOR.PROGRAM /usr/lib/emacs/etc/emacsclient
set_def NOTE_EDITOR.PROGRAM ""
set_def ALIGNMENT.OPEN.MIN 0
set_def ALIGNMENT.OPEN.MAX 20
set_def ALIGNMENT.EXTEND.MIN 0
set_def ALIGNMENT.EXTEND.MAX 20
#consistency plot
set_def CONSISTENCY_DISPLAY.WIN .consistency_win
set_def CONSISTENCY_DISPLAY.RULER $defs_ruler
set_def CONSISTENCY_DISPLAY.BORDERWIDTH 2
set_def CONSISTENCY_DISPLAY.CURSOR .cursor_t
set_def CONSISTENCY_DISPLAY.CURSOR_COLOUR "black"
set_def CONSISTENCY_DISPLAY.CURSOR_WIDTH 0
set_def CONSISTENCY_DISPLAY.PLOT_WIDTH 700
set_def CONSISTENCY_DISPLAY.CURSOR1 .cursor_t
set_def CONSISTENCY_DISPLAY.CURSOR2 .cursor_l
set_def CONSISTENCY_DISPLAY.CURSORY .cursor_y
set_def CONSISTENCY_DISPLAY.PADX 5
#confidence graph
set_def CONFIDENCE_GRAPH.COLOUR "black"
set_def CONFIDENCE_GRAPH.WIN .confidence_graph_win
set_def CONFIDENCE_GRAPH.PLOT_HEIGHT 200
set_def CONFIDENCE_GRAPH.BORDERWIDTH 2
set_def CONFIDENCE_GRAPH.INFILE $defs_c_in
set_def CONFIDENCE_GRAPH.INFILE.NAME.VALUE "contigs"
set_def CONFIDENCE_GRAPH.LINEWIDTH 2
set_def CONFIDENCE_GRAPH.PADY 5
set_def CONFIDENCE_GRAPH.RULER $defs_ruler_v
set_def CONFIDENCE_GRAPH.RULER.OFFSET 40
set_def CONFIDENCE_GRAPH.RULER.TEXT_OFFSET 20
set_def CONFIDENCE_GRAPH.PLOT_TYPE line
#reading coverage
set_def READING_COVERAGE.WIN .reading_coverage_win
set_def READING_COVERAGE.PLOT_HEIGHT 200
set_def READING_COVERAGE.BORDERWIDTH 2
set_def READING_COVERAGE.INFILE $defs_c_in
set_def READING_COVERAGE.INFILE.NAME.VALUE "contigs"
set_def READING_COVERAGE.LINEWIDTH 2
set_def READING_COVERAGE.PADY 5
set_def READING_COVERAGE.RULER $defs_ruler_v
set_def READING_COVERAGE.RULER.OFFSET 40
set_def READING_COVERAGE.RULER.TEXT_OFFSET 20
set_def READING_COVERAGE.STRAND.NAME "Select strand"
set_def READING_COVERAGE.STRAND.VALUE 3
set_def READING_COVERAGE.STRAND.BUTTON.1 "Forward strand"
set_def READING_COVERAGE.STRAND.BUTTON.2 "Reverse strand"
set_def READING_COVERAGE.STRAND.BUTTON.3 "Both strands"
set_def READING_COVERAGE.STRAND.BUTTON.4 "Summation"
set_def READING_COVERAGE.COLOUR1 red
set_def READING_COVERAGE.COLOUR2 black
# Template display (some also in READING.* above)
set_def TEMPLATE.QUAL_COLOUR yellow4
set_def TEMPLATE.HETERO_COLOUR magenta
set_def TEMPLATE.DISCREP_COLOUR green
#readpair coverage
set_def READPAIR_COVERAGE.COLOUR "black"
set_def READPAIR_COVERAGE.WIN .readpair_coverage_win
set_def READPAIR_COVERAGE.PLOT_HEIGHT 200
set_def READPAIR_COVERAGE.BORDERWIDTH 2
set_def READPAIR_COVERAGE.INFILE $defs_c_in
set_def READPAIR_COVERAGE.INFILE.NAME.VALUE "contigs"
set_def READPAIR_COVERAGE.LINEWIDTH 2
set_def READPAIR_COVERAGE.PADY 5
set_def READPAIR_COVERAGE.RULER $defs_ruler_v
set_def READPAIR_COVERAGE.RULER.OFFSET 40
set_def READPAIR_COVERAGE.RULER.TEXT_OFFSET 20
#strand coverage
set_def STRAND_COVERAGE.COLOUR1 red
set_def STRAND_COVERAGE.COLOUR2 black
set_def STRAND_COVERAGE.WIN .strand_coverage_win
set_def STRAND_COVERAGE.PLOT_HEIGHT 50
set_def STRAND_COVERAGE.BORDERWIDTH 2
set_def STRAND_COVERAGE.INFILE $defs_c_in
set_def STRAND_COVERAGE.INFILE.NAME.VALUE "contigs"
set_def STRAND_COVERAGE.LINEWIDTH 2
set_def STRAND_COVERAGE.PADY 5
set_def STRAND_COVERAGE.STRAND.NAME "Select strand"
set_def STRAND_COVERAGE.STRAND.VALUE 3
set_def STRAND_COVERAGE.STRAND.BUTTON.1 "Positive strand"
set_def STRAND_COVERAGE.STRAND.BUTTON.2 "Reverse strand"
set_def STRAND_COVERAGE.STRAND.BUTTON.3 "Both strands"
set_def STRAND_COVERAGE.PROBLEM.NAME "Select plot type"
set_def STRAND_COVERAGE.PROBLEM.VALUE 2
set_def STRAND_COVERAGE.PROBLEM.BUTTON.1 "Coverage"
set_def STRAND_COVERAGE.PROBLEM.BUTTON.2 "Problems"
set_def STRAND_COVERAGE.FORWARD_OFFSET 10
set_def STRAND_COVERAGE.REVERSE_OFFSET 30
set_def SEARCH_CASE 1
set_def SEARCH_MODE 2
set_def SEARCH_LIST_SEQUENCE sequence_id
set_def SEARCH_LIST_TEMPLATE template
set_def SEARCH_LIST_ANNOTATION tags
# Contig navigator
set_def CONTIG_NAVIGATOR.AUTO_CLOSE 1
set_def CONTIG_NAVIGATOR.SHOW_TRACES 1
# Prefinish
set_def PREFINISH.CONFIG_FILE $env(STADTABL)/config.prefinish
# fasta/fastq assembly
set_def ASSEMBLE_SINGLE.FORMAT 1
# Check Database
set_def CHECK_DATABASE.INFILE $defs_c_in
set_def CHECK_DATABASE.FIX 0
set_def CHECK_DATABASE.DETAIL 2; # 2=>Thorough, 1=>Fast
# Extend Contigs
set_def EXTEND_CONTIGS.INFILE $defs_c_in
set_def EXTEND_CONTIGS.MIN_DEPTH 5
set_def EXTEND_CONTIGS.MATCH_SCORE 1
set_def EXTEND_CONTIGS.MISMATCH_SCORE -3
set_def EXTEND_CONTIGS.TRIM_DEPTH 3
# Bulk Contig Rename
set_def CONTIG_BULK_RENAME.INFILE $defs_c_in
set_def CONTIG_BULK_RENAME.INFILE.WHICH.BUTTONS {list file pattern}
set_def CONTIG_BULK_RENAME.INFILE.WHICH.VALUE 3
set_def CONTIG_BULK_RENAME.SEARCH *
set_def CONTIG_BULK_RENAME.REPLACE Contig_%06d
set_def CONTIG_BULK_RENAME.INDEX 1
# Set this to a Tcl list of the interpreter names of programs which Gap4 can
# communicate with using the Send To command. If they're not already running,
# it'll start one itself with the second half of the name. NB, interpreter
# names not listed here, but containing an EventHandler function will still be
# listed.
# Eg "set_def GAP_SEND_TO {{Prog1 prog1} {Prog2 {/bin/prog2 -s}}}"
# set_def GAP_SEND_TO {{Spin spin}}
load_menus
set_menu gap_menu
#if {[keylget gap5_defs MENU_FILE] != "viewer"} {
# load_package %L/cap2 {} cap2 0
# load_package %L/cap3 {} cap3 0
# load_package %L/fak2 {} fak2 0
# load_package %L/phrap {} phrap 0
#}
#load_package haplo
|