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1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 | LOCUS H45989 495 bp mRNA linear EST 31-JUL-1995
DEFINITION yo13c02.s1 Soares adult brain N2b5HB55Y Homo sapiens cDNA clone
IMAGE:177794 3', mRNA sequence.
ACCESSION H45989
VERSION H45989.1 GI:922041
KEYWORDS EST.
SOURCE Homo sapiens (human)
ORGANISM Homo sapiens
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
Catarrhini; Hominidae; Homo.
REFERENCE 1 (bases 1 to 495)
AUTHORS Hillier,L., Clark,N., Dubuque,T., Elliston,K., Hawkins,M.,
Holman,M., Hultman,M., Kucaba,T., Le,M., Lennon,G., Marra,M.,
Parsons,J., Rifkin,L., Rohlfing,T., Soares,M., Tan,F.,
Trevaskis,E., Waterston,R., Williamson,A., Wohldmann,P. and
Wilson,R.
TITLE The WashU-Merck EST Project
JOURNAL Unpublished (1995)
COMMENT Contact: Wilson RK
Washington University School of Medicine
4444 Forest Park Parkway, Box 8501, St. Louis, MO 63108
Tel: 314 286 1800
Fax: 314 286 1810
Email: est@watson.wustl.edu
Insert Size: 544
High quality sequence stops: 265
Source: IMAGE Consortium, LLNL
This clone is available royalty-free through LLNL ; contact the
IMAGE Consortium (info@image.llnl.gov) for further information.
Possible reversed clone: polyT not found
Insert Length: 544 Std Error: 0.00
Seq primer: SP6
High quality sequence stop: 265.
FEATURES Location/Qualifiers
source 1. .495
/organism="Homo sapiens"
/mol_type="mRNA"
/db_xref="GDB:3839990"
/db_xref="taxon:9606"
/clone="IMAGE:177794"
/sex="Male"
/dev_stage="55-year old"
/lab_host="DH10B (ampicillin resistant)"
/clone_lib="Soares adult brain N2b5HB55Y"
/note="Organ: brain; Vector: pT7T3D (Pharmacia) with a
modified polylinker; Site_1: Not I; Site_2: Eco RI; 1st
strand cDNA was primed with a Not I - oligo(dT) primer [5'
TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTTTTTTTTTTTTTTTTTTT 3'],
double-stranded cDNA was size selected, ligated to Eco RI
adapters (Pharmacia), digested with Not I and cloned into
the Not I and Eco RI sites of a modified pT7T3 vector
(Pharmacia). Library went through one round of
normalization to a Cot = 53. Library constructed by Bento
Soares and M.Fatima Bonaldo. The adult brain RNA was
provided by Dr. Donald H. Gilden. Tissue was acquired
17-18 hours after death which occurred in consequence of a
ruptured aortic aneurysm. RNA was prepared from a pool of
tissues representing the following areas of the brain:
frontal, parietal, temporal and occipital cortex from the
left and right hemispheres, subcortical white matter,
basal ganglia, thalamus, cerebellum, midbrain, pons and
medulla."
ORIGIN
1 CCGGNAAGCT CANCTTGGAC CACCGACTCT CGANTGNNTC GCCGCGGGAG CCGGNTGGAN
61 AACCTGAGCG GGACTGGNAG AAGGAGCAGA GGGAGGCAGC ACCCGGCGTG ACGGNAGTGT
121 GTGGGGCACT CAGGCCTTCC GCAGTGTCAT CTGCCACACG GAAGGCACGG CCACGGGCAG
181 GGGGGTCTAT GATCTTCTGC ATGCCCAGCT GGCATGGCCC CACGTAGAGT GGNNTGGCGT
241 CTCGGTGCTG GTCAGCGACA CGTTGTCCTG GCTGGGCAGG TCCAGCTCCC GGAGGACCTG
301 GGGCTTCAGC TTCCCGTAGC GCTGGCTGCA GTGACGGATG CTCTTGCGCT GCCATTTCTG
361 GGTGCTGTCA CTGTCCTTGC TCACTCCAAA CCAGTTCGGC GGTCCCCCTG CGGATGGTCT
421 GTGTTGATGG ACGTTTGGGC TTTGCAGCAC CGGCCGCCGA GTTCATGGTN GGGTNAAGAG
481 ATTTGGGTTT TTTCN
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