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/usr/lib/R/site-library/SummarizedExperiment/doc/SummarizedExperiment.R is in r-bioc-summarizedexperiment 1.8.0-1.

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## ----style, echo=FALSE, results='asis'-------------------------------------
BiocStyle::markdown()

## ----include = FALSE-------------------------------------------------------
# download current version of SE diagram
#download.file("https://docs.google.com/feeds/download/drawings/Export?id=18OcDb80FpvSGRYnFl-8vUqwNNLaNHrG1I9SWKHCselo&exportFormat=svg", "SE.svg")
download.file("https://docs.google.com/feeds/download/drawings/Export?id=1kiC8Qlo1mhSnLDqkGiRNPSo6GWn3C2duBszCFbJCB-g&exportFormat=svg", "SE.svg")

## ---- echo=FALSE-----------------------------------------------------------
suppressPackageStartupMessages(library(SummarizedExperiment))
suppressPackageStartupMessages(data(airway, package="airway"))

## --------------------------------------------------------------------------
library(SummarizedExperiment)
data(airway, package="airway")
se <- airway
se

## ----assays, eval = FALSE--------------------------------------------------
#  assays(se)$counts

## ----assays_table, echo = FALSE--------------------------------------------
knitr::kable(assays(se)$counts[1:10,])

## ----rowRanges-------------------------------------------------------------
rowRanges(se)

## ----colData---------------------------------------------------------------
colData(se)

## ----columnSubset----------------------------------------------------------
# subset for only those samples treated with dexamethasone
se[, se$dex == "trt"]

## ----metadata--------------------------------------------------------------
metadata(se)

## ----metadata-formula------------------------------------------------------
metadata(se)$formula <- counts ~ dex + albut

metadata(se)

## ----constructRSE----------------------------------------------------------
nrows <- 200
ncols <- 6
counts <- matrix(runif(nrows * ncols, 1, 1e4), nrows)
rowRanges <- GRanges(rep(c("chr1", "chr2"), c(50, 150)),
                     IRanges(floor(runif(200, 1e5, 1e6)), width=100),
                     strand=sample(c("+", "-"), 200, TRUE),
                     feature_id=sprintf("ID%03d", 1:200))
colData <- DataFrame(Treatment=rep(c("ChIP", "Input"), 3),
                     row.names=LETTERS[1:6])

SummarizedExperiment(assays=list(counts=counts),
                     rowRanges=rowRanges, colData=colData)

## ----constructSE-----------------------------------------------------------
SummarizedExperiment(assays=list(counts=counts), colData=colData)

## ----2d--------------------------------------------------------------------
# subset the first five transcripts and first three samples
se[1:5, 1:3]

## ----colDataExtraction-----------------------------------------------------
se[, se$cell == "N61311"]

## ----getSet----------------------------------------------------------------
counts <- matrix(1:15, 5, 3, dimnames=list(LETTERS[1:5], LETTERS[1:3]))

dates <- SummarizedExperiment(assays=list(counts=counts),
                              rowData=DataFrame(month=month.name[1:5], day=1:5))

# Subset all January assays
dates[rowData(dates)$month == "January", ]

## ----assay_assays----------------------------------------------------------
assays(se)

assays(se)[[1]][1:5, 1:5]

# assay defaults to the first assay if no i is given
assay(se)[1:5, 1:5]

assay(se, 1)[1:5, 1:5]

## ----overlap---------------------------------------------------------------
# Subset for only rows which are in the interval 100,000 to 110,000 of
# chromosome 1
roi <- GRanges(seqnames="1", ranges=100000:1100000)
subsetByOverlaps(se, roi)

## ----rseSubclass-----------------------------------------------------------
setClass("MyRSESubclass",
    contains="RangedSummarizedExperiment",
    representation=representation(
        slot1="integer",
        slot2="function"
        ## ... maybe more ...
    )
)