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All rights reserved.
# This code is part of the Biopython distribution and governed by its
# license. Please see the LICENSE file that should have been included
# as part of this package.
# Patches by Mike Poidinger to support multiple databases.
# Updated by Peter Cock in 2007 to do a better job on BLAST 2.2.15
"""Code for calling standalone BLAST and parsing plain text output (OBSOLETE).
Rather than parsing the human readable plain text BLAST output (which seems to
change with every update to BLAST), we and the NBCI recommend you parse the
XML output instead. The plain text parser in this module still works at the
time of writing, but is considered obsolete and updating it to cope with the
latest versions of BLAST is not a priority for us.
This module also provides code to work with the "legacy" standalone version of
NCBI BLAST, tools blastall, rpsblast and blastpgp via three helper functions of
the same name. These functions are very limited for dealing with the output as
files rather than handles, for which the wrappers in Bio.Blast.Applications are
prefered. Furthermore, the NCBI themselves regard these command line tools as
"legacy", and encourage using the new BLAST+ tools instead. Biopython has
wrappers for these under Bio.Blast.Applications (see the tutorial).
Classes:
LowQualityBlastError Except that indicates low quality query sequences.
BlastParser Parses output from blast.
BlastErrorParser Parses output and tries to diagnose possible errors.
PSIBlastParser Parses output from psi-blast.
Iterator Iterates over a file of blast results.
_Scanner Scans output from standalone BLAST.
_BlastConsumer Consumes output from blast.
_PSIBlastConsumer Consumes output from psi-blast.
_HeaderConsumer Consumes header information.
_DescriptionConsumer Consumes description information.
_AlignmentConsumer Consumes alignment information.
_HSPConsumer Consumes hsp information.
_DatabaseReportConsumer Consumes database report information.
_ParametersConsumer Consumes parameters information.
Functions:
blastall Execute blastall (OBSOLETE).
blastpgp Execute blastpgp (OBSOLETE).
rpsblast Execute rpsblast (OBSOLETE).
For calling the BLAST command line tools, we encourage you to use the
command line wrappers in Bio.Blast.Applications - the three functions
blastall, blastpgp and rpsblast are considered to be obsolete now, and
are likely to be deprecated and then removed in future releases.
"""
import warnings
warnings.warn("The plain text parser in this module still works at the time of writing, but is considered obsolete and updating it to cope with the latest versions of BLAST is not a priority for us.", PendingDeprecationWarning)
import os
import re
from Bio import File
from Bio.ParserSupport import *
from Bio.Blast import Record
from Bio.Application import _escape_filename
class LowQualityBlastError(Exception):
"""Error caused by running a low quality sequence through BLAST.
When low quality sequences (like GenBank entries containing only
stretches of a single nucleotide) are BLASTed, they will result in
BLAST generating an error and not being able to perform the BLAST.
search. This error should be raised for the BLAST reports produced
in this case.
"""
pass
class ShortQueryBlastError(Exception):
"""Error caused by running a short query sequence through BLAST.
If the query sequence is too short, BLAST outputs warnings and errors:
Searching[blastall] WARNING: [000.000] AT1G08320: SetUpBlastSearch failed.
[blastall] ERROR: [000.000] AT1G08320: Blast:
[blastall] ERROR: [000.000] AT1G08320: Blast: Query must be at least wordsize
done
This exception is raised when that condition is detected.
"""
pass
class _Scanner:
"""Scan BLAST output from blastall or blastpgp.
Tested with blastall and blastpgp v2.0.10, v2.0.11
Methods:
feed Feed data into the scanner.
"""
def feed(self, handle, consumer):
"""S.feed(handle, consumer)
Feed in a BLAST report for scanning. handle is a file-like
object that contains the BLAST report. consumer is a Consumer
object that will receive events as the report is scanned.
"""
if isinstance(handle, File.UndoHandle):
uhandle = handle
else:
uhandle = File.UndoHandle(handle)
# Try to fast-forward to the beginning of the blast report.
read_and_call_until(uhandle, consumer.noevent, contains='BLAST')
# Now scan the BLAST report.
self._scan_header(uhandle, consumer)
self._scan_rounds(uhandle, consumer)
self._scan_database_report(uhandle, consumer)
self._scan_parameters(uhandle, consumer)
def _scan_header(self, uhandle, consumer):
# BLASTP 2.0.10 [Aug-26-1999]
#
#
# Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaf
# Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
# "Gapped BLAST and PSI-BLAST: a new generation of protein database sea
# programs", Nucleic Acids Res. 25:3389-3402.
#
# Query= test
# (140 letters)
#
# Database: sdqib40-1.35.seg.fa
# 1323 sequences; 223,339 total letters
#
# ========================================================
# This next example is from the online version of Blast,
# note there are TWO references, an RID line, and also
# the database is BEFORE the query line.
# Note there possibleuse of non-ASCII in the author names.
# ========================================================
#
# BLASTP 2.2.15 [Oct-15-2006]
# Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Sch??ffer,
# Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman
# (1997), "Gapped BLAST and PSI-BLAST: a new generation of
# protein database search programs", Nucleic Acids Res. 25:3389-3402.
#
# Reference: Sch??ffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei
# Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and
# Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST
# protein database searches with composition-based statistics
# and other refinements", Nucleic Acids Res. 29:2994-3005.
#
# RID: 1166022616-19998-65316425856.BLASTQ1
#
#
# Database: All non-redundant GenBank CDS
# translations+PDB+SwissProt+PIR+PRF excluding environmental samples
# 4,254,166 sequences; 1,462,033,012 total letters
# Query= gi:16127998
# Length=428
#
consumer.start_header()
read_and_call(uhandle, consumer.version, contains='BLAST')
read_and_call_while(uhandle, consumer.noevent, blank=1)
# There might be a <pre> line, for qblast output.
attempt_read_and_call(uhandle, consumer.noevent, start="<pre>")
# Read the reference(s)
while attempt_read_and_call(uhandle,
consumer.reference, start='Reference'):
# References are normally multiline terminated by a blank line
# (or, based on the old code, the RID line)
while 1:
line = uhandle.readline()
if is_blank_line(line):
consumer.noevent(line)
break
elif line.startswith("RID"):
break
else:
#More of the reference
consumer.reference(line)
#Deal with the optional RID: ...
read_and_call_while(uhandle, consumer.noevent, blank=1)
attempt_read_and_call(uhandle, consumer.reference, start="RID:")
read_and_call_while(uhandle, consumer.noevent, blank=1)
# blastpgp may have a reference for compositional score matrix
# adjustment (see Bug 2502):
if attempt_read_and_call(
uhandle, consumer.reference, start="Reference"):
read_and_call_until(uhandle, consumer.reference, blank=1)
read_and_call_while(uhandle, consumer.noevent, blank=1)
# blastpgp has a Reference for composition-based statistics.
if attempt_read_and_call(
uhandle, consumer.reference, start="Reference"):
read_and_call_until(uhandle, consumer.reference, blank=1)
read_and_call_while(uhandle, consumer.noevent, blank=1)
line = uhandle.peekline()
assert line.strip() != ""
assert not line.startswith("RID:")
if line.startswith("Query="):
#This is an old style query then database...
# Read the Query lines and the following blank line.
read_and_call(uhandle, consumer.query_info, start='Query=')
read_and_call_until(uhandle, consumer.query_info, blank=1)
read_and_call_while(uhandle, consumer.noevent, blank=1)
# Read the database lines and the following blank line.
read_and_call_until(uhandle, consumer.database_info, end='total letters')
read_and_call(uhandle, consumer.database_info, contains='sequences')
read_and_call_while(uhandle, consumer.noevent, blank=1)
elif line.startswith("Database:"):
#This is a new style database then query...
read_and_call_until(uhandle, consumer.database_info, end='total letters')
read_and_call(uhandle, consumer.database_info, contains='sequences')
read_and_call_while(uhandle, consumer.noevent, blank=1)
# Read the Query lines and the following blank line.
# Or, on BLAST 2.2.22+ there is no blank link - need to spot
# the "... Score E" line instead.
read_and_call(uhandle, consumer.query_info, start='Query=')
#read_and_call_until(uhandle, consumer.query_info, blank=1)
while True:
line = uhandle.peekline()
if not line.strip() : break
if "Score E" in line : break
#It is more of the query (and its length)
read_and_call(uhandle, consumer.query_info)
read_and_call_while(uhandle, consumer.noevent, blank=1)
else:
raise ValueError("Invalid header?")
consumer.end_header()
def _scan_rounds(self, uhandle, consumer):
# Scan a bunch of rounds.
# Each round begins with either a "Searching......" line
# or a 'Score E' line followed by descriptions and alignments.
# The email server doesn't give the "Searching....." line.
# If there is no 'Searching.....' line then you'll first see a
# 'Results from round' line
while not self._eof(uhandle):
line = safe_peekline(uhandle)
if (not line.startswith('Searching') and
not line.startswith('Results from round') and
re.search(r"Score +E", line) is None and
line.find('No hits found') == -1):
break
self._scan_descriptions(uhandle, consumer)
self._scan_alignments(uhandle, consumer)
def _scan_descriptions(self, uhandle, consumer):
# Searching..................................................done
# Results from round 2
#
#
# Sc
# Sequences producing significant alignments: (b
# Sequences used in model and found again:
#
# d1tde_2 3.4.1.4.4 (119-244) Thioredoxin reductase [Escherichia ...
# d1tcob_ 1.31.1.5.16 Calcineurin regulatory subunit (B-chain) [B...
# d1symb_ 1.31.1.2.2 Calcyclin (S100) [RAT (RATTUS NORVEGICUS)]
#
# Sequences not found previously or not previously below threshold:
#
# d1osa__ 1.31.1.5.11 Calmodulin [Paramecium tetraurelia]
# d1aoza3 2.5.1.3.3 (339-552) Ascorbate oxidase [zucchini (Cucurb...
#
# If PSI-BLAST, may also have:
#
# CONVERGED!
consumer.start_descriptions()
# Read 'Searching'
# This line seems to be missing in BLASTN 2.1.2 (others?)
attempt_read_and_call(uhandle, consumer.noevent, start='Searching')
# blastpgp 2.0.10 from NCBI 9/19/99 for Solaris sometimes crashes here.
# If this happens, the handle will yield no more information.
if not uhandle.peekline():
raise ValueError("Unexpected end of blast report. " + \
"Looks suspiciously like a PSI-BLAST crash.")
# BLASTN 2.2.3 sometimes spews a bunch of warnings and errors here:
# Searching[blastall] WARNING: [000.000] AT1G08320: SetUpBlastSearch
# [blastall] ERROR: [000.000] AT1G08320: Blast:
# [blastall] ERROR: [000.000] AT1G08320: Blast: Query must be at leas
# done
# Reported by David Weisman.
# Check for these error lines and ignore them for now. Let
# the BlastErrorParser deal with them.
line = uhandle.peekline()
if line.find("ERROR:") != -1 or line.startswith("done"):
read_and_call_while(uhandle, consumer.noevent, contains="ERROR:")
read_and_call(uhandle, consumer.noevent, start="done")
# Check to see if this is PSI-BLAST.
# If it is, the 'Searching' line will be followed by:
# (version 2.0.10)
# Searching.............................
# Results from round 2
# or (version 2.0.11)
# Searching.............................
#
#
# Results from round 2
# Skip a bunch of blank lines.
read_and_call_while(uhandle, consumer.noevent, blank=1)
# Check for the results line if it's there.
if attempt_read_and_call(uhandle, consumer.round, start='Results'):
read_and_call_while(uhandle, consumer.noevent, blank=1)
# Three things can happen here:
# 1. line contains 'Score E'
# 2. line contains "No hits found"
# 3. no descriptions
# The first one begins a bunch of descriptions. The last two
# indicates that no descriptions follow, and we should go straight
# to the alignments.
if not attempt_read_and_call(
uhandle, consumer.description_header,
has_re=re.compile(r'Score +E')):
# Either case 2 or 3. Look for "No hits found".
attempt_read_and_call(uhandle, consumer.no_hits,
contains='No hits found')
try:
read_and_call_while(uhandle, consumer.noevent, blank=1)
except ValueError, err:
if str(err) != "Unexpected end of stream." : raise err
consumer.end_descriptions()
# Stop processing.
return
# Read the score header lines
read_and_call(uhandle, consumer.description_header,
start='Sequences producing')
# If PSI-BLAST, read the 'Sequences used in model' line.
attempt_read_and_call(uhandle, consumer.model_sequences,
start='Sequences used in model')
read_and_call_while(uhandle, consumer.noevent, blank=1)
# In BLAT, rather than a "No hits found" line, we just
# get no descriptions (and no alignments). This can be
# spotted because the next line is the database block:
if safe_peekline(uhandle).startswith(" Database:"):
consumer.end_descriptions()
# Stop processing.
return
# Read the descriptions and the following blank lines, making
# sure that there are descriptions.
if not uhandle.peekline().startswith('Sequences not found'):
read_and_call_until(uhandle, consumer.description, blank=1)
read_and_call_while(uhandle, consumer.noevent, blank=1)
# If PSI-BLAST, read the 'Sequences not found' line followed
# by more descriptions. However, I need to watch out for the
# case where there were no sequences not found previously, in
# which case there will be no more descriptions.
if attempt_read_and_call(uhandle, consumer.nonmodel_sequences,
start='Sequences not found'):
# Read the descriptions and the following blank lines.
read_and_call_while(uhandle, consumer.noevent, blank=1)
l = safe_peekline(uhandle)
# Brad -- added check for QUERY. On some PSI-BLAST outputs
# there will be a 'Sequences not found' line followed by no
# descriptions. Check for this case since the first thing you'll
# get is a blank line and then 'QUERY'
if not l.startswith('CONVERGED') and l[0] != '>' \
and not l.startswith('QUERY'):
read_and_call_until(uhandle, consumer.description, blank=1)
read_and_call_while(uhandle, consumer.noevent, blank=1)
attempt_read_and_call(uhandle, consumer.converged, start='CONVERGED')
read_and_call_while(uhandle, consumer.noevent, blank=1)
consumer.end_descriptions()
def _scan_alignments(self, uhandle, consumer):
if self._eof(uhandle) : return
# qblast inserts a helpful line here.
attempt_read_and_call(uhandle, consumer.noevent, start="ALIGNMENTS")
# First, check to see if I'm at the database report.
line = safe_peekline(uhandle)
if not line:
#EOF
return
elif line.startswith(' Database') or line.startswith("Lambda"):
return
elif line[0] == '>':
# XXX make a better check here between pairwise and masterslave
self._scan_pairwise_alignments(uhandle, consumer)
else:
# XXX put in a check to make sure I'm in a masterslave alignment
self._scan_masterslave_alignment(uhandle, consumer)
def _scan_pairwise_alignments(self, uhandle, consumer):
while not self._eof(uhandle):
line = safe_peekline(uhandle)
if line[0] != '>':
break
self._scan_one_pairwise_alignment(uhandle, consumer)
def _scan_one_pairwise_alignment(self, uhandle, consumer):
if self._eof(uhandle) : return
consumer.start_alignment()
self._scan_alignment_header(uhandle, consumer)
# Scan a bunch of score/alignment pairs.
while 1:
if self._eof(uhandle):
#Shouldn't have issued that _scan_alignment_header event...
break
line = safe_peekline(uhandle)
if not line.startswith(' Score'):
break
self._scan_hsp(uhandle, consumer)
consumer.end_alignment()
def _scan_alignment_header(self, uhandle, consumer):
# >d1rip__ 2.24.7.1.1 Ribosomal S17 protein [Bacillus
# stearothermophilus]
# Length = 81
#
# Or, more recently with different white space:
#
# >gi|15799684|ref|NP_285696.1| threonine synthase ...
# gi|15829258|ref|NP_308031.1| threonine synthase
# ...
# Length=428
read_and_call(uhandle, consumer.title, start='>')
while 1:
line = safe_readline(uhandle)
if line.lstrip().startswith('Length =') \
or line.lstrip().startswith('Length='):
consumer.length(line)
break
elif is_blank_line(line):
# Check to make sure I haven't missed the Length line
raise ValueError("I missed the Length in an alignment header")
consumer.title(line)
# Older versions of BLAST will have a line with some spaces.
# Version 2.0.14 (maybe 2.0.13?) and above print a true blank line.
if not attempt_read_and_call(uhandle, consumer.noevent,
start=' '):
read_and_call(uhandle, consumer.noevent, blank=1)
def _scan_hsp(self, uhandle, consumer):
consumer.start_hsp()
self._scan_hsp_header(uhandle, consumer)
self._scan_hsp_alignment(uhandle, consumer)
consumer.end_hsp()
def _scan_hsp_header(self, uhandle, consumer):
# Score = 22.7 bits (47), Expect = 2.5
# Identities = 10/36 (27%), Positives = 18/36 (49%)
# Strand = Plus / Plus
# Frame = +3
#
read_and_call(uhandle, consumer.score, start=' Score')
read_and_call(uhandle, consumer.identities, start=' Identities')
# BLASTN
attempt_read_and_call(uhandle, consumer.strand, start = ' Strand')
# BLASTX, TBLASTN, TBLASTX
attempt_read_and_call(uhandle, consumer.frame, start = ' Frame')
read_and_call(uhandle, consumer.noevent, blank=1)
def _scan_hsp_alignment(self, uhandle, consumer):
# Query: 11 GRGVSACA-------TCDGFFYRNQKVAVIGGGNTAVEEALYLSNIASEVHLIHRRDGF
# GRGVS+ TC Y + + V GGG+ + EE L + I R+
# Sbjct: 12 GRGVSSVVRRCIHKPTCKE--YAVKIIDVTGGGSFSAEEVQELREATLKEVDILRKVSG
#
# Query: 64 AEKILIKR 71
# I +K
# Sbjct: 70 PNIIQLKD 77
#
while 1:
# Blastn adds an extra line filled with spaces before Query
attempt_read_and_call(uhandle, consumer.noevent, start=' ')
read_and_call(uhandle, consumer.query, start='Query')
read_and_call(uhandle, consumer.align, start=' ')
read_and_call(uhandle, consumer.sbjct, start='Sbjct')
try:
read_and_call_while(uhandle, consumer.noevent, blank=1)
except ValueError, err:
if str(err) != "Unexpected end of stream." : raise err
# End of File (well, it looks like it with recent versions
# of BLAST for multiple queries after the Iterator class
# has broken up the whole file into chunks).
break
line = safe_peekline(uhandle)
# Alignment continues if I see a 'Query' or the spaces for Blastn.
if not (line.startswith('Query') or line.startswith(' ')):
break
def _scan_masterslave_alignment(self, uhandle, consumer):
consumer.start_alignment()
while 1:
line = safe_readline(uhandle)
# Check to see whether I'm finished reading the alignment.
# This is indicated by 1) database section, 2) next psi-blast
# round, which can also be a 'Results from round' if no
# searching line is present
# patch by chapmanb
if line.startswith('Searching') or \
line.startswith('Results from round'):
uhandle.saveline(line)
break
elif line.startswith(' Database'):
uhandle.saveline(line)
break
elif is_blank_line(line):
consumer.noevent(line)
else:
consumer.multalign(line)
read_and_call_while(uhandle, consumer.noevent, blank=1)
consumer.end_alignment()
def _eof(self, uhandle):
try:
line = safe_peekline(uhandle)
except ValueError, err:
if str(err) != "Unexpected end of stream." : raise err
line = ""
return not line
def _scan_database_report(self, uhandle, consumer):
# Database: sdqib40-1.35.seg.fa
# Posted date: Nov 1, 1999 4:25 PM
# Number of letters in database: 223,339
# Number of sequences in database: 1323
#
# Lambda K H
# 0.322 0.133 0.369
#
# Gapped
# Lambda K H
# 0.270 0.0470 0.230
#
##########################################
# Or, more recently Blast 2.2.15 gives less blank lines
##########################################
# Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF excluding
# environmental samples
# Posted date: Dec 12, 2006 5:51 PM
# Number of letters in database: 667,088,753
# Number of sequences in database: 2,094,974
# Lambda K H
# 0.319 0.136 0.395
# Gapped
# Lambda K H
# 0.267 0.0410 0.140
if self._eof(uhandle) : return
consumer.start_database_report()
# Subset of the database(s) listed below
# Number of letters searched: 562,618,960
# Number of sequences searched: 228,924
if attempt_read_and_call(uhandle, consumer.noevent, start=" Subset"):
read_and_call(uhandle, consumer.noevent, contains="letters")
read_and_call(uhandle, consumer.noevent, contains="sequences")
read_and_call(uhandle, consumer.noevent, start=" ")
# Sameet Mehta reported seeing output from BLASTN 2.2.9 that
# was missing the "Database" stanza completely.
while attempt_read_and_call(uhandle, consumer.database,
start=' Database'):
# BLAT output ends abruptly here, without any of the other
# information. Check to see if this is the case. If so,
# then end the database report here gracefully.
if not uhandle.peekline().strip() \
or uhandle.peekline().startswith("BLAST"):
consumer.end_database_report()
return
# Database can span multiple lines.
read_and_call_until(uhandle, consumer.database, start=' Posted')
read_and_call(uhandle, consumer.posted_date, start=' Posted')
read_and_call(uhandle, consumer.num_letters_in_database,
start=' Number of letters')
read_and_call(uhandle, consumer.num_sequences_in_database,
start=' Number of sequences')
#There may not be a line starting with spaces...
attempt_read_and_call(uhandle, consumer.noevent, start=' ')
line = safe_readline(uhandle)
uhandle.saveline(line)
if line.find('Lambda') != -1:
break
read_and_call(uhandle, consumer.noevent, start='Lambda')
read_and_call(uhandle, consumer.ka_params)
#This blank line is optional:
attempt_read_and_call(uhandle, consumer.noevent, blank=1)
# not BLASTP
attempt_read_and_call(uhandle, consumer.gapped, start='Gapped')
# not TBLASTX
if attempt_read_and_call(uhandle, consumer.noevent, start='Lambda'):
read_and_call(uhandle, consumer.ka_params_gap)
# Blast 2.2.4 can sometimes skip the whole parameter section.
# Thus, I need to be careful not to read past the end of the
# file.
try:
read_and_call_while(uhandle, consumer.noevent, blank=1)
except ValueError, x:
if str(x) != "Unexpected end of stream.":
raise
consumer.end_database_report()
def _scan_parameters(self, uhandle, consumer):
# Matrix: BLOSUM62
# Gap Penalties: Existence: 11, Extension: 1
# Number of Hits to DB: 50604
# Number of Sequences: 1323
# Number of extensions: 1526
# Number of successful extensions: 6
# Number of sequences better than 10.0: 5
# Number of HSP's better than 10.0 without gapping: 5
# Number of HSP's successfully gapped in prelim test: 0
# Number of HSP's that attempted gapping in prelim test: 1
# Number of HSP's gapped (non-prelim): 5
# length of query: 140
# length of database: 223,339
# effective HSP length: 39
# effective length of query: 101
# effective length of database: 171,742
# effective search space: 17345942
# effective search space used: 17345942
# T: 11
# A: 40
# X1: 16 ( 7.4 bits)
# X2: 38 (14.8 bits)
# X3: 64 (24.9 bits)
# S1: 41 (21.9 bits)
# S2: 42 (20.8 bits)
##########################################
# Or, more recently Blast(x) 2.2.15 gives
##########################################
# Matrix: BLOSUM62
# Gap Penalties: Existence: 11, Extension: 1
# Number of Sequences: 4535438
# Number of Hits to DB: 2,588,844,100
# Number of extensions: 60427286
# Number of successful extensions: 126433
# Number of sequences better than 2.0: 30
# Number of HSP's gapped: 126387
# Number of HSP's successfully gapped: 35
# Length of query: 291
# Length of database: 1,573,298,872
# Length adjustment: 130
# Effective length of query: 161
# Effective length of database: 983,691,932
# Effective search space: 158374401052
# Effective search space used: 158374401052
# Neighboring words threshold: 12
# Window for multiple hits: 40
# X1: 16 ( 7.3 bits)
# X2: 38 (14.6 bits)
# X3: 64 (24.7 bits)
# S1: 41 (21.7 bits)
# S2: 32 (16.9 bits)
# Blast 2.2.4 can sometimes skip the whole parameter section.
# BLAT also skips the whole parameter section.
# Thus, check to make sure that the parameter section really
# exists.
if not uhandle.peekline().strip():
return
# BLASTN 2.2.9 looks like it reverses the "Number of Hits" and
# "Number of Sequences" lines.
consumer.start_parameters()
# Matrix line may be missing in BLASTN 2.2.9
attempt_read_and_call(uhandle, consumer.matrix, start='Matrix')
# not TBLASTX
attempt_read_and_call(uhandle, consumer.gap_penalties, start='Gap')
attempt_read_and_call(uhandle, consumer.num_sequences,
start='Number of Sequences')
attempt_read_and_call(uhandle, consumer.num_hits,
start='Number of Hits')
attempt_read_and_call(uhandle, consumer.num_sequences,
start='Number of Sequences')
attempt_read_and_call(uhandle, consumer.num_extends,
start='Number of extensions')
attempt_read_and_call(uhandle, consumer.num_good_extends,
start='Number of successful')
attempt_read_and_call(uhandle, consumer.num_seqs_better_e,
start='Number of sequences')
# not BLASTN, TBLASTX
if attempt_read_and_call(uhandle, consumer.hsps_no_gap,
start="Number of HSP's better"):
# BLASTN 2.2.9
if attempt_read_and_call(uhandle, consumer.noevent,
start="Number of HSP's gapped:"):
read_and_call(uhandle, consumer.noevent,
start="Number of HSP's successfully")
#This is ommitted in 2.2.15
attempt_read_and_call(uhandle, consumer.noevent,
start="Number of extra gapped extensions")
else:
read_and_call(uhandle, consumer.hsps_prelim_gapped,
start="Number of HSP's successfully")
read_and_call(uhandle, consumer.hsps_prelim_gap_attempted,
start="Number of HSP's that")
read_and_call(uhandle, consumer.hsps_gapped,
start="Number of HSP's gapped")
#e.g. BLASTX 2.2.15 where the "better" line is missing
elif attempt_read_and_call(uhandle, consumer.noevent,
start="Number of HSP's gapped"):
read_and_call(uhandle, consumer.noevent,
start="Number of HSP's successfully")
# not in blastx 2.2.1
attempt_read_and_call(uhandle, consumer.query_length,
has_re=re.compile(r"[Ll]ength of query"))
# Not in BLASTX 2.2.22+
attempt_read_and_call(uhandle, consumer.database_length,
has_re=re.compile(r"[Ll]ength of \s*[Dd]atabase"))
# BLASTN 2.2.9
attempt_read_and_call(uhandle, consumer.noevent,
start="Length adjustment")
attempt_read_and_call(uhandle, consumer.effective_hsp_length,
start='effective HSP')
# Not in blastx 2.2.1
attempt_read_and_call(
uhandle, consumer.effective_query_length,
has_re=re.compile(r'[Ee]ffective length of query'))
# This is not in BLASTP 2.2.15
attempt_read_and_call(
uhandle, consumer.effective_database_length,
has_re=re.compile(r'[Ee]ffective length of \s*[Dd]atabase'))
# Not in blastx 2.2.1, added a ':' to distinguish between
# this and the 'effective search space used' line
attempt_read_and_call(
uhandle, consumer.effective_search_space,
has_re=re.compile(r'[Ee]ffective search space:'))
# Does not appear in BLASTP 2.0.5
attempt_read_and_call(
uhandle, consumer.effective_search_space_used,
has_re=re.compile(r'[Ee]ffective search space used'))
# BLASTX, TBLASTN, TBLASTX
attempt_read_and_call(uhandle, consumer.frameshift, start='frameshift')
# not in BLASTN 2.2.9
attempt_read_and_call(uhandle, consumer.threshold, start='T')
# In BLASTX 2.2.15 replaced by: "Neighboring words threshold: 12"
attempt_read_and_call(uhandle, consumer.threshold, start='Neighboring words threshold')
# not in BLASTX 2.2.15
attempt_read_and_call(uhandle, consumer.window_size, start='A')
# get this instead: "Window for multiple hits: 40"
attempt_read_and_call(uhandle, consumer.window_size, start='Window for multiple hits')
# not in BLASTX 2.2.22+
attempt_read_and_call(uhandle, consumer.dropoff_1st_pass, start='X1')
# not TBLASTN
attempt_read_and_call(uhandle, consumer.gap_x_dropoff, start='X2')
# not BLASTN, TBLASTX
attempt_read_and_call(uhandle, consumer.gap_x_dropoff_final,
start='X3')
# not TBLASTN
attempt_read_and_call(uhandle, consumer.gap_trigger, start='S1')
# not in blastx 2.2.1
# first we make sure we have additional lines to work with, if
# not then the file is done and we don't have a final S2
if not is_blank_line(uhandle.peekline(), allow_spaces=1):
read_and_call(uhandle, consumer.blast_cutoff, start='S2')
consumer.end_parameters()
class BlastParser(AbstractParser):
"""Parses BLAST data into a Record.Blast object.
"""
def __init__(self):
"""__init__(self)"""
self._scanner = _Scanner()
self._consumer = _BlastConsumer()
def parse(self, handle):
"""parse(self, handle)"""
self._scanner.feed(handle, self._consumer)
return self._consumer.data
class PSIBlastParser(AbstractParser):
"""Parses BLAST data into a Record.PSIBlast object.
"""
def __init__(self):
"""__init__(self)"""
self._scanner = _Scanner()
self._consumer = _PSIBlastConsumer()
def parse(self, handle):
"""parse(self, handle)"""
self._scanner.feed(handle, self._consumer)
return self._consumer.data
class _HeaderConsumer:
def start_header(self):
self._header = Record.Header()
def version(self, line):
c = line.split()
self._header.application = c[0]
self._header.version = c[1]
if len(c) > 2:
#The date is missing in the new C++ output from blastx 2.2.22+
#Just get "BLASTX 2.2.22+\n" and that's all.
self._header.date = c[2][1:-1]
def reference(self, line):
if line.startswith('Reference: '):
self._header.reference = line[11:]
else:
self._header.reference = self._header.reference + line
def query_info(self, line):
if line.startswith('Query= '):
self._header.query = line[7:].lstrip()
elif line.startswith('Length='):
#New style way to give the query length in BLAST 2.2.22+ (the C++ code)
self._header.query_letters = _safe_int(line[7:].strip())
elif not line.startswith(' '): # continuation of query_info
self._header.query = "%s%s" % (self._header.query, line)
else:
#Hope it is the old style way to give the query length:
letters, = _re_search(
r"([0-9,]+) letters", line,
"I could not find the number of letters in line\n%s" % line)
self._header.query_letters = _safe_int(letters)
def database_info(self, line):
line = line.rstrip()
if line.startswith('Database: '):
self._header.database = line[10:]
elif not line.endswith('total letters'):
if self._header.database:
#Need to include a space when merging multi line datase descr
self._header.database = self._header.database + " " + line.strip()
else:
self._header.database = line.strip()
else:
sequences, letters =_re_search(
r"([0-9,]+) sequences; ([0-9,-]+) total letters", line,
"I could not find the sequences and letters in line\n%s" %line)
self._header.database_sequences = _safe_int(sequences)
self._header.database_letters = _safe_int(letters)
def end_header(self):
# Get rid of the trailing newlines
self._header.reference = self._header.reference.rstrip()
self._header.query = self._header.query.rstrip()
class _DescriptionConsumer:
def start_descriptions(self):
self._descriptions = []
self._model_sequences = []
self._nonmodel_sequences = []
self._converged = 0
self._type = None
self._roundnum = None
self.__has_n = 0 # Does the description line contain an N value?
def description_header(self, line):
if line.startswith('Sequences producing'):
cols = line.split()
if cols[-1] == 'N':
self.__has_n = 1
def description(self, line):
dh = self._parse(line)
if self._type == 'model':
self._model_sequences.append(dh)
elif self._type == 'nonmodel':
self._nonmodel_sequences.append(dh)
else:
self._descriptions.append(dh)
def model_sequences(self, line):
self._type = 'model'
def nonmodel_sequences(self, line):
self._type = 'nonmodel'
def converged(self, line):
self._converged = 1
def no_hits(self, line):
pass
def round(self, line):
if not line.startswith('Results from round'):
raise ValueError("I didn't understand the round line\n%s" % line)
self._roundnum = _safe_int(line[18:].strip())
def end_descriptions(self):
pass
def _parse(self, description_line):
line = description_line # for convenience
dh = Record.Description()
# I need to separate the score and p-value from the title.
# sp|P21297|FLBT_CAUCR FLBT PROTEIN [snip] 284 7e-77
# sp|P21297|FLBT_CAUCR FLBT PROTEIN [snip] 284 7e-77 1
# special cases to handle:
# - title must be preserved exactly (including whitespaces)
# - score could be equal to e-value (not likely, but what if??)
# - sometimes there's an "N" score of '1'.
cols = line.split()
if len(cols) < 3:
raise ValueError( \
"Line does not appear to contain description:\n%s" % line)
if self.__has_n:
i = line.rfind(cols[-1]) # find start of N
i = line.rfind(cols[-2], 0, i) # find start of p-value
i = line.rfind(cols[-3], 0, i) # find start of score
else:
i = line.rfind(cols[-1]) # find start of p-value
i = line.rfind(cols[-2], 0, i) # find start of score
if self.__has_n:
dh.title, dh.score, dh.e, dh.num_alignments = \
line[:i].rstrip(), cols[-3], cols[-2], cols[-1]
else:
dh.title, dh.score, dh.e, dh.num_alignments = \
line[:i].rstrip(), cols[-2], cols[-1], 1
dh.num_alignments = _safe_int(dh.num_alignments)
dh.score = _safe_int(dh.score)
dh.e = _safe_float(dh.e)
return dh
class _AlignmentConsumer:
# This is a little bit tricky. An alignment can either be a
# pairwise alignment or a multiple alignment. Since it's difficult
# to know a-priori which one the blast record will contain, I'm going
# to make one class that can parse both of them.
def start_alignment(self):
self._alignment = Record.Alignment()
self._multiple_alignment = Record.MultipleAlignment()
def title(self, line):
if self._alignment.title:
self._alignment.title += " "
self._alignment.title += line.strip()
def length(self, line):
#e.g. "Length = 81" or more recently, "Length=428"
parts = line.replace(" ","").split("=")
assert len(parts)==2, "Unrecognised format length line"
self._alignment.length = parts[1]
self._alignment.length = _safe_int(self._alignment.length)
def multalign(self, line):
# Standalone version uses 'QUERY', while WWW version uses blast_tmp.
if line.startswith('QUERY') or line.startswith('blast_tmp'):
# If this is the first line of the multiple alignment,
# then I need to figure out how the line is formatted.
# Format of line is:
# QUERY 1 acttg...gccagaggtggtttattcagtctccataagagaggggacaaacg 60
try:
name, start, seq, end = line.split()
except ValueError:
raise ValueError("I do not understand the line\n%s" % line)
self._start_index = line.index(start, len(name))
self._seq_index = line.index(seq,
self._start_index+len(start))
# subtract 1 for the space
self._name_length = self._start_index - 1
self._start_length = self._seq_index - self._start_index - 1
self._seq_length = line.rfind(end) - self._seq_index - 1
#self._seq_index = line.index(seq)
## subtract 1 for the space
#self._seq_length = line.rfind(end) - self._seq_index - 1
#self._start_index = line.index(start)
#self._start_length = self._seq_index - self._start_index - 1
#self._name_length = self._start_index
# Extract the information from the line
name = line[:self._name_length]
name = name.rstrip()
start = line[self._start_index:self._start_index+self._start_length]
start = start.rstrip()
if start:
start = _safe_int(start)
end = line[self._seq_index+self._seq_length:].rstrip()
if end:
end = _safe_int(end)
seq = line[self._seq_index:self._seq_index+self._seq_length].rstrip()
# right pad the sequence with spaces if necessary
if len(seq) < self._seq_length:
seq = seq + ' '*(self._seq_length-len(seq))
# I need to make sure the sequence is aligned correctly with the query.
# First, I will find the length of the query. Then, if necessary,
# I will pad my current sequence with spaces so that they will line
# up correctly.
# Two possible things can happen:
# QUERY
# 504
#
# QUERY
# 403
#
# Sequence 504 will need padding at the end. Since I won't know
# this until the end of the alignment, this will be handled in
# end_alignment.
# Sequence 403 will need padding before being added to the alignment.
align = self._multiple_alignment.alignment # for convenience
align.append((name, start, seq, end))
# This is old code that tried to line up all the sequences
# in a multiple alignment by using the sequence title's as
# identifiers. The problem with this is that BLAST assigns
# different HSP's from the same sequence the same id. Thus,
# in one alignment block, there may be multiple sequences with
# the same id. I'm not sure how to handle this, so I'm not
# going to.
# # If the sequence is the query, then just add it.
# if name == 'QUERY':
# if len(align) == 0:
# align.append((name, start, seq))
# else:
# aname, astart, aseq = align[0]
# if name != aname:
# raise ValueError, "Query is not the first sequence"
# aseq = aseq + seq
# align[0] = aname, astart, aseq
# else:
# if len(align) == 0:
# raise ValueError, "I could not find the query sequence"
# qname, qstart, qseq = align[0]
#
# # Now find my sequence in the multiple alignment.
# for i in range(1, len(align)):
# aname, astart, aseq = align[i]
# if name == aname:
# index = i
# break
# else:
# # If I couldn't find it, then add a new one.
# align.append((None, None, None))
# index = len(align)-1
# # Make sure to left-pad it.
# aname, astart, aseq = name, start, ' '*(len(qseq)-len(seq))
#
# if len(qseq) != len(aseq) + len(seq):
# # If my sequences are shorter than the query sequence,
# # then I will need to pad some spaces to make them line up.
# # Since I've already right padded seq, that means aseq
# # must be too short.
# aseq = aseq + ' '*(len(qseq)-len(aseq)-len(seq))
# aseq = aseq + seq
# if astart is None:
# astart = start
# align[index] = aname, astart, aseq
def end_alignment(self):
# Remove trailing newlines
if self._alignment:
self._alignment.title = self._alignment.title.rstrip()
# This code is also obsolete. See note above.
# If there's a multiple alignment, I will need to make sure
# all the sequences are aligned. That is, I may need to
# right-pad the sequences.
# if self._multiple_alignment is not None:
# align = self._multiple_alignment.alignment
# seqlen = None
# for i in range(len(align)):
# name, start, seq = align[i]
# if seqlen is None:
# seqlen = len(seq)
# else:
# if len(seq) < seqlen:
# seq = seq + ' '*(seqlen - len(seq))
# align[i] = name, start, seq
# elif len(seq) > seqlen:
# raise ValueError, \
# "Sequence %s is longer than the query" % name
# Clean up some variables, if they exist.
try:
del self._seq_index
del self._seq_length
del self._start_index
del self._start_length
del self._name_length
except AttributeError:
pass
class _HSPConsumer:
def start_hsp(self):
self._hsp = Record.HSP()
def score(self, line):
self._hsp.bits, self._hsp.score = _re_search(
r"Score =\s*([0-9.e+]+) bits \(([0-9]+)\)", line,
"I could not find the score in line\n%s" % line)
self._hsp.score = _safe_float(self._hsp.score)
self._hsp.bits = _safe_float(self._hsp.bits)
x, y = _re_search(
r"Expect\(?(\d*)\)? = +([0-9.e\-|\+]+)", line,
"I could not find the expect in line\n%s" % line)
if x:
self._hsp.num_alignments = _safe_int(x)
else:
self._hsp.num_alignments = 1
self._hsp.expect = _safe_float(y)
def identities(self, line):
x, y = _re_search(
r"Identities = (\d+)\/(\d+)", line,
"I could not find the identities in line\n%s" % line)
self._hsp.identities = _safe_int(x), _safe_int(y)
self._hsp.align_length = _safe_int(y)
if line.find('Positives') != -1:
x, y = _re_search(
r"Positives = (\d+)\/(\d+)", line,
"I could not find the positives in line\n%s" % line)
self._hsp.positives = _safe_int(x), _safe_int(y)
assert self._hsp.align_length == _safe_int(y)
if line.find('Gaps') != -1:
x, y = _re_search(
r"Gaps = (\d+)\/(\d+)", line,
"I could not find the gaps in line\n%s" % line)
self._hsp.gaps = _safe_int(x), _safe_int(y)
assert self._hsp.align_length == _safe_int(y)
def strand(self, line):
self._hsp.strand = _re_search(
r"Strand = (\w+) / (\w+)", line,
"I could not find the strand in line\n%s" % line)
def frame(self, line):
# Frame can be in formats:
# Frame = +1
# Frame = +2 / +2
if line.find('/') != -1:
self._hsp.frame = _re_search(
r"Frame = ([-+][123]) / ([-+][123])", line,
"I could not find the frame in line\n%s" % line)
else:
self._hsp.frame = _re_search(
r"Frame = ([-+][123])", line,
"I could not find the frame in line\n%s" % line)
# Match a space, if one is available. Masahir Ishikawa found a
# case where there's no space between the start and the sequence:
# Query: 100tt 101
# line below modified by Yair Benita, Sep 2004
# Note that the colon is not always present. 2006
_query_re = re.compile(r"Query(:?) \s*(\d+)\s*(.+) (\d+)")
def query(self, line):
m = self._query_re.search(line)
if m is None:
raise ValueError("I could not find the query in line\n%s" % line)
# line below modified by Yair Benita, Sep 2004.
# added the end attribute for the query
colon, start, seq, end = m.groups()
self._hsp.query = self._hsp.query + seq
if self._hsp.query_start is None:
self._hsp.query_start = _safe_int(start)
# line below added by Yair Benita, Sep 2004.
# added the end attribute for the query
self._hsp.query_end = _safe_int(end)
#Get index for sequence start (regular expression element 3)
self._query_start_index = m.start(3)
self._query_len = len(seq)
def align(self, line):
seq = line[self._query_start_index:].rstrip()
if len(seq) < self._query_len:
# Make sure the alignment is the same length as the query
seq = seq + ' ' * (self._query_len-len(seq))
elif len(seq) < self._query_len:
raise ValueError("Match is longer than the query in line\n%s" \
% line)
self._hsp.match = self._hsp.match + seq
# To match how we do the query, cache the regular expression.
# Note that the colon is not always present.
_sbjct_re = re.compile(r"Sbjct(:?) \s*(\d+)\s*(.+) (\d+)")
def sbjct(self, line):
m = self._sbjct_re.search(line)
if m is None:
raise ValueError("I could not find the sbjct in line\n%s" % line)
colon, start, seq, end = m.groups()
#mikep 26/9/00
#On occasion, there is a blast hit with no subject match
#so far, it only occurs with 1-line short "matches"
#I have decided to let these pass as they appear
if not seq.strip():
seq = ' ' * self._query_len
self._hsp.sbjct = self._hsp.sbjct + seq
if self._hsp.sbjct_start is None:
self._hsp.sbjct_start = _safe_int(start)
self._hsp.sbjct_end = _safe_int(end)
if len(seq) != self._query_len:
raise ValueError( \
"QUERY and SBJCT sequence lengths don't match in line\n%s" \
% line)
del self._query_start_index # clean up unused variables
del self._query_len
def end_hsp(self):
pass
class _DatabaseReportConsumer:
def start_database_report(self):
self._dr = Record.DatabaseReport()
def database(self, line):
m = re.search(r"Database: (.+)$", line)
if m:
self._dr.database_name.append(m.group(1))
elif self._dr.database_name:
# This must be a continuation of the previous name.
self._dr.database_name[-1] = "%s%s" % (self._dr.database_name[-1],
line.strip())
def posted_date(self, line):
self._dr.posted_date.append(_re_search(
r"Posted date:\s*(.+)$", line,
"I could not find the posted date in line\n%s" % line))
def num_letters_in_database(self, line):
letters, = _get_cols(
line, (-1,), ncols=6, expected={2:"letters", 4:"database:"})
self._dr.num_letters_in_database.append(_safe_int(letters))
def num_sequences_in_database(self, line):
sequences, = _get_cols(
line, (-1,), ncols=6, expected={2:"sequences", 4:"database:"})
self._dr.num_sequences_in_database.append(_safe_int(sequences))
def ka_params(self, line):
x = line.split()
self._dr.ka_params = map(_safe_float, x)
def gapped(self, line):
self._dr.gapped = 1
def ka_params_gap(self, line):
x = line.split()
self._dr.ka_params_gap = map(_safe_float, x)
def end_database_report(self):
pass
class _ParametersConsumer:
def start_parameters(self):
self._params = Record.Parameters()
def matrix(self, line):
self._params.matrix = line[8:].rstrip()
def gap_penalties(self, line):
x = _get_cols(
line, (3, 5), ncols=6, expected={2:"Existence:", 4:"Extension:"})
self._params.gap_penalties = map(_safe_float, x)
def num_hits(self, line):
if line.find('1st pass') != -1:
x, = _get_cols(line, (-4,), ncols=11, expected={2:"Hits"})
self._params.num_hits = _safe_int(x)
else:
x, = _get_cols(line, (-1,), ncols=6, expected={2:"Hits"})
self._params.num_hits = _safe_int(x)
def num_sequences(self, line):
if line.find('1st pass') != -1:
x, = _get_cols(line, (-4,), ncols=9, expected={2:"Sequences:"})
self._params.num_sequences = _safe_int(x)
else:
x, = _get_cols(line, (-1,), ncols=4, expected={2:"Sequences:"})
self._params.num_sequences = _safe_int(x)
def num_extends(self, line):
if line.find('1st pass') != -1:
x, = _get_cols(line, (-4,), ncols=9, expected={2:"extensions:"})
self._params.num_extends = _safe_int(x)
else:
x, = _get_cols(line, (-1,), ncols=4, expected={2:"extensions:"})
self._params.num_extends = _safe_int(x)
def num_good_extends(self, line):
if line.find('1st pass') != -1:
x, = _get_cols(line, (-4,), ncols=10, expected={3:"extensions:"})
self._params.num_good_extends = _safe_int(x)
else:
x, = _get_cols(line, (-1,), ncols=5, expected={3:"extensions:"})
self._params.num_good_extends = _safe_int(x)
def num_seqs_better_e(self, line):
self._params.num_seqs_better_e, = _get_cols(
line, (-1,), ncols=7, expected={2:"sequences"})
self._params.num_seqs_better_e = _safe_int(
self._params.num_seqs_better_e)
def hsps_no_gap(self, line):
self._params.hsps_no_gap, = _get_cols(
line, (-1,), ncols=9, expected={3:"better", 7:"gapping:"})
self._params.hsps_no_gap = _safe_int(self._params.hsps_no_gap)
def hsps_prelim_gapped(self, line):
self._params.hsps_prelim_gapped, = _get_cols(
line, (-1,), ncols=9, expected={4:"gapped", 6:"prelim"})
self._params.hsps_prelim_gapped = _safe_int(
self._params.hsps_prelim_gapped)
def hsps_prelim_gapped_attempted(self, line):
self._params.hsps_prelim_gapped_attempted, = _get_cols(
line, (-1,), ncols=10, expected={4:"attempted", 7:"prelim"})
self._params.hsps_prelim_gapped_attempted = _safe_int(
self._params.hsps_prelim_gapped_attempted)
def hsps_gapped(self, line):
self._params.hsps_gapped, = _get_cols(
line, (-1,), ncols=6, expected={3:"gapped"})
self._params.hsps_gapped = _safe_int(self._params.hsps_gapped)
def query_length(self, line):
self._params.query_length, = _get_cols(
line.lower(), (-1,), ncols=4, expected={0:"length", 2:"query:"})
self._params.query_length = _safe_int(self._params.query_length)
def database_length(self, line):
self._params.database_length, = _get_cols(
line.lower(), (-1,), ncols=4, expected={0:"length", 2:"database:"})
self._params.database_length = _safe_int(self._params.database_length)
def effective_hsp_length(self, line):
self._params.effective_hsp_length, = _get_cols(
line, (-1,), ncols=4, expected={1:"HSP", 2:"length:"})
self._params.effective_hsp_length = _safe_int(
self._params.effective_hsp_length)
def effective_query_length(self, line):
self._params.effective_query_length, = _get_cols(
line, (-1,), ncols=5, expected={1:"length", 3:"query:"})
self._params.effective_query_length = _safe_int(
self._params.effective_query_length)
def effective_database_length(self, line):
self._params.effective_database_length, = _get_cols(
line.lower(), (-1,), ncols=5, expected={1:"length", 3:"database:"})
self._params.effective_database_length = _safe_int(
self._params.effective_database_length)
def effective_search_space(self, line):
self._params.effective_search_space, = _get_cols(
line, (-1,), ncols=4, expected={1:"search"})
self._params.effective_search_space = _safe_int(
self._params.effective_search_space)
def effective_search_space_used(self, line):
self._params.effective_search_space_used, = _get_cols(
line, (-1,), ncols=5, expected={1:"search", 3:"used:"})
self._params.effective_search_space_used = _safe_int(
self._params.effective_search_space_used)
def frameshift(self, line):
self._params.frameshift = _get_cols(
line, (4, 5), ncols=6, expected={0:"frameshift", 2:"decay"})
def threshold(self, line):
if line[:2] == "T:":
#Assume its an old stlye line like "T: 123"
self._params.threshold, = _get_cols(
line, (1,), ncols=2, expected={0:"T:"})
elif line[:28] == "Neighboring words threshold:":
self._params.threshold, = _get_cols(
line, (3,), ncols=4, expected={0:"Neighboring", 1:"words", 2:"threshold:"})
else:
raise ValueError("Unrecognised threshold line:\n%s" % line)
self._params.threshold = _safe_int(self._params.threshold)
def window_size(self, line):
if line[:2] == "A:":
self._params.window_size, = _get_cols(
line, (1,), ncols=2, expected={0:"A:"})
elif line[:25] == "Window for multiple hits:":
self._params.window_size, = _get_cols(
line, (4,), ncols=5, expected={0:"Window", 2:"multiple", 3:"hits:"})
else:
raise ValueError("Unrecognised window size line:\n%s" % line)
self._params.window_size = _safe_int(self._params.window_size)
def dropoff_1st_pass(self, line):
score, bits = _re_search(
r"X1: (\d+) \(\s*([0-9,.]+) bits\)", line,
"I could not find the dropoff in line\n%s" % line)
self._params.dropoff_1st_pass = _safe_int(score), _safe_float(bits)
def gap_x_dropoff(self, line):
score, bits = _re_search(
r"X2: (\d+) \(\s*([0-9,.]+) bits\)", line,
"I could not find the gap dropoff in line\n%s" % line)
self._params.gap_x_dropoff = _safe_int(score), _safe_float(bits)
def gap_x_dropoff_final(self, line):
score, bits = _re_search(
r"X3: (\d+) \(\s*([0-9,.]+) bits\)", line,
"I could not find the gap dropoff final in line\n%s" % line)
self._params.gap_x_dropoff_final = _safe_int(score), _safe_float(bits)
def gap_trigger(self, line):
score, bits = _re_search(
r"S1: (\d+) \(\s*([0-9,.]+) bits\)", line,
"I could not find the gap trigger in line\n%s" % line)
self._params.gap_trigger = _safe_int(score), _safe_float(bits)
def blast_cutoff(self, line):
score, bits = _re_search(
r"S2: (\d+) \(\s*([0-9,.]+) bits\)", line,
"I could not find the blast cutoff in line\n%s" % line)
self._params.blast_cutoff = _safe_int(score), _safe_float(bits)
def end_parameters(self):
pass
class _BlastConsumer(AbstractConsumer,
_HeaderConsumer,
_DescriptionConsumer,
_AlignmentConsumer,
_HSPConsumer,
_DatabaseReportConsumer,
_ParametersConsumer
):
# This Consumer is inherits from many other consumer classes that handle
# the actual dirty work. An alternate way to do it is to create objects
# of those classes and then delegate the parsing tasks to them in a
# decorator-type pattern. The disadvantage of that is that the method
# names will need to be resolved in this classes. However, using
# a decorator will retain more control in this class (which may or
# may not be a bad thing). In addition, having each sub-consumer as
# its own object prevents this object's dictionary from being cluttered
# with members and reduces the chance of member collisions.
def __init__(self):
self.data = None
def round(self, line):
# Make sure nobody's trying to pass me PSI-BLAST data!
raise ValueError("This consumer doesn't handle PSI-BLAST data")
def start_header(self):
self.data = Record.Blast()
_HeaderConsumer.start_header(self)
def end_header(self):
_HeaderConsumer.end_header(self)
self.data.__dict__.update(self._header.__dict__)
def end_descriptions(self):
self.data.descriptions = self._descriptions
def end_alignment(self):
_AlignmentConsumer.end_alignment(self)
if self._alignment.hsps:
self.data.alignments.append(self._alignment)
if self._multiple_alignment.alignment:
self.data.multiple_alignment = self._multiple_alignment
def end_hsp(self):
_HSPConsumer.end_hsp(self)
try:
self._alignment.hsps.append(self._hsp)
except AttributeError:
raise ValueError("Found an HSP before an alignment")
def end_database_report(self):
_DatabaseReportConsumer.end_database_report(self)
self.data.__dict__.update(self._dr.__dict__)
def end_parameters(self):
_ParametersConsumer.end_parameters(self)
self.data.__dict__.update(self._params.__dict__)
class _PSIBlastConsumer(AbstractConsumer,
_HeaderConsumer,
_DescriptionConsumer,
_AlignmentConsumer,
_HSPConsumer,
_DatabaseReportConsumer,
_ParametersConsumer
):
def __init__(self):
self.data = None
def start_header(self):
self.data = Record.PSIBlast()
_HeaderConsumer.start_header(self)
def end_header(self):
_HeaderConsumer.end_header(self)
self.data.__dict__.update(self._header.__dict__)
def start_descriptions(self):
self._round = Record.Round()
self.data.rounds.append(self._round)
_DescriptionConsumer.start_descriptions(self)
def end_descriptions(self):
_DescriptionConsumer.end_descriptions(self)
self._round.number = self._roundnum
if self._descriptions:
self._round.new_seqs.extend(self._descriptions)
self._round.reused_seqs.extend(self._model_sequences)
self._round.new_seqs.extend(self._nonmodel_sequences)
if self._converged:
self.data.converged = 1
def end_alignment(self):
_AlignmentConsumer.end_alignment(self)
if self._alignment.hsps:
self._round.alignments.append(self._alignment)
if self._multiple_alignment:
self._round.multiple_alignment = self._multiple_alignment
def end_hsp(self):
_HSPConsumer.end_hsp(self)
try:
self._alignment.hsps.append(self._hsp)
except AttributeError:
raise ValueError("Found an HSP before an alignment")
def end_database_report(self):
_DatabaseReportConsumer.end_database_report(self)
self.data.__dict__.update(self._dr.__dict__)
def end_parameters(self):
_ParametersConsumer.end_parameters(self)
self.data.__dict__.update(self._params.__dict__)
class Iterator(object):
"""Iterates over a file of multiple BLAST results.
Methods:
next Return the next record from the stream, or None.
"""
def __init__(self, handle, parser=None):
"""__init__(self, handle, parser=None)
Create a new iterator. handle is a file-like object. parser
is an optional Parser object to change the results into another form.
If set to None, then the raw contents of the file will be returned.
"""
try:
handle.readline
except AttributeError:
raise ValueError(
"I expected a file handle or file-like object, got %s"
% type(handle))
self._uhandle = File.UndoHandle(handle)
self._parser = parser
self._header = []
def next(self):
"""next(self) -> object
Return the next Blast record from the file. If no more records,
return None.
"""
lines = []
query = False
while 1:
line = self._uhandle.readline()
if not line:
break
# If I've reached the next one, then put the line back and stop.
if lines and (line.startswith('BLAST')
or line.startswith('BLAST', 1)
or line.startswith('<?xml ')):
self._uhandle.saveline(line)
break
# New style files ommit the BLAST line to mark a new query:
if line.startswith("Query="):
if not query:
if not self._header:
self._header = lines[:]
query = True
else:
#Start of another record
self._uhandle.saveline(line)
break
lines.append(line)
if query and "BLAST" not in lines[0]:
#Cheat and re-insert the header
#print "-"*50
#print "".join(self._header)
#print "-"*50
#print "".join(lines)
#print "-"*50
lines = self._header + lines
if not lines:
return None
data = ''.join(lines)
if self._parser is not None:
return self._parser.parse(File.StringHandle(data))
return data
def __iter__(self):
return iter(self.next, None)
def blastall(blastcmd, program, database, infile, align_view='7', **keywds):
"""Execute and retrieve data from standalone BLASTPALL as handles (OBSOLETE).
NOTE - This function is obsolete, you are encouraged to the command
line wrapper Bio.Blast.Applications.BlastallCommandline instead.
Execute and retrieve data from blastall. blastcmd is the command
used to launch the 'blastall' executable. program is the blast program
to use, e.g. 'blastp', 'blastn', etc. database is the path to the database
to search against. infile is the path to the file containing
the sequence to search with.
The return values are two handles, for standard output and standard error.
You may pass more parameters to **keywds to change the behavior of
the search. Otherwise, optional values will be chosen by blastall.
The Blast output is by default in XML format. Use the align_view keyword
for output in a different format.
Scoring
matrix Matrix to use.
gap_open Gap open penalty.
gap_extend Gap extension penalty.
nuc_match Nucleotide match reward. (BLASTN)
nuc_mismatch Nucleotide mismatch penalty. (BLASTN)
query_genetic_code Genetic code for Query.
db_genetic_code Genetic code for database. (TBLAST[NX])
Algorithm
gapped Whether to do a gapped alignment. T/F (not for TBLASTX)
expectation Expectation value cutoff.
wordsize Word size.
strands Query strands to search against database.([T]BLAST[NX])
keep_hits Number of best hits from a region to keep.
xdrop Dropoff value (bits) for gapped alignments.
hit_extend Threshold for extending hits.
region_length Length of region used to judge hits.
db_length Effective database length.
search_length Effective length of search space.
Processing
filter Filter query sequence for low complexity (with SEG)? T/F
believe_query Believe the query defline. T/F
restrict_gi Restrict search to these GI's.
nprocessors Number of processors to use.
oldengine Force use of old engine T/F
Formatting
html Produce HTML output? T/F
descriptions Number of one-line descriptions.
alignments Number of alignments.
align_view Alignment view. Integer 0-11,
passed as a string or integer.
show_gi Show GI's in deflines? T/F
seqalign_file seqalign file to output.
outfile Output file for report. Filename to write to, if
ommitted standard output is used (which you can access
from the returned handles).
"""
_security_check_parameters(keywds)
att2param = {
'matrix' : '-M',
'gap_open' : '-G',
'gap_extend' : '-E',
'nuc_match' : '-r',
'nuc_mismatch' : '-q',
'query_genetic_code' : '-Q',
'db_genetic_code' : '-D',
'gapped' : '-g',
'expectation' : '-e',
'wordsize' : '-W',
'strands' : '-S',
'keep_hits' : '-K',
'xdrop' : '-X',
'hit_extend' : '-f',
'region_length' : '-L',
'db_length' : '-z',
'search_length' : '-Y',
'program' : '-p',
'database' : '-d',
'infile' : '-i',
'filter' : '-F',
'believe_query' : '-J',
'restrict_gi' : '-l',
'nprocessors' : '-a',
'oldengine' : '-V',
'html' : '-T',
'descriptions' : '-v',
'alignments' : '-b',
'align_view' : '-m',
'show_gi' : '-I',
'seqalign_file' : '-O',
'outfile' : '-o',
}
import warnings
warnings.warn("This function is obsolete, you are encouraged to the command line wrapper Bio.Blast.Applications.BlastallCommandline instead.", PendingDeprecationWarning)
from Applications import BlastallCommandline
cline = BlastallCommandline(blastcmd)
cline.set_parameter(att2param['program'], program)
cline.set_parameter(att2param['database'], database)
cline.set_parameter(att2param['infile'], infile)
cline.set_parameter(att2param['align_view'], str(align_view))
for key, value in keywds.iteritems():
cline.set_parameter(att2param[key], str(value))
return _invoke_blast(cline)
def blastpgp(blastcmd, database, infile, align_view='7', **keywds):
"""Execute and retrieve data from standalone BLASTPGP as handles (OBSOLETE).
NOTE - This function is obsolete, you are encouraged to the command
line wrapper Bio.Blast.Applications.BlastpgpCommandline instead.
Execute and retrieve data from blastpgp. blastcmd is the command
used to launch the 'blastpgp' executable. database is the path to the
database to search against. infile is the path to the file containing
the sequence to search with.
The return values are two handles, for standard output and standard error.
You may pass more parameters to **keywds to change the behavior of
the search. Otherwise, optional values will be chosen by blastpgp.
The Blast output is by default in XML format. Use the align_view keyword
for output in a different format.
Scoring
matrix Matrix to use.
gap_open Gap open penalty.
gap_extend Gap extension penalty.
window_size Multiple hits window size.
npasses Number of passes.
passes Hits/passes. Integer 0-2.
Algorithm
gapped Whether to do a gapped alignment. T/F
expectation Expectation value cutoff.
wordsize Word size.
keep_hits Number of beset hits from a region to keep.
xdrop Dropoff value (bits) for gapped alignments.
hit_extend Threshold for extending hits.
region_length Length of region used to judge hits.
db_length Effective database length.
search_length Effective length of search space.
nbits_gapping Number of bits to trigger gapping.
pseudocounts Pseudocounts constants for multiple passes.
xdrop_final X dropoff for final gapped alignment.
xdrop_extension Dropoff for blast extensions.
model_threshold E-value threshold to include in multipass model.
required_start Start of required region in query.
required_end End of required region in query.
Processing
XXX should document default values
program The blast program to use. (PHI-BLAST)
filter Filter query sequence for low complexity (with SEG)? T/F
believe_query Believe the query defline? T/F
nprocessors Number of processors to use.
Formatting
html Produce HTML output? T/F
descriptions Number of one-line descriptions.
alignments Number of alignments.
align_view Alignment view. Integer 0-11,
passed as a string or integer.
show_gi Show GI's in deflines? T/F
seqalign_file seqalign file to output.
align_outfile Output file for alignment.
checkpoint_outfile Output file for PSI-BLAST checkpointing.
restart_infile Input file for PSI-BLAST restart.
hit_infile Hit file for PHI-BLAST.
matrix_outfile Output file for PSI-BLAST matrix in ASCII.
align_outfile Output file for alignment. Filename to write to, if
ommitted standard output is used (which you can access
from the returned handles).
align_infile Input alignment file for PSI-BLAST restart.
"""
import warnings
warnings.warn("This function is obsolete, you are encouraged to the command line wrapper Bio.Blast.Applications.BlastpgpCommandline instead.", PendingDeprecationWarning)
_security_check_parameters(keywds)
att2param = {
'matrix' : '-M',
'gap_open' : '-G',
'gap_extend' : '-E',
'window_size' : '-A',
'npasses' : '-j',
'passes' : '-P',
'gapped' : '-g',
'expectation' : '-e',
'wordsize' : '-W',
'keep_hits' : '-K',
'xdrop' : '-X',
'hit_extend' : '-f',
'region_length' : '-L',
'db_length' : '-Z',
'search_length' : '-Y',
'nbits_gapping' : '-N',
'pseudocounts' : '-c',
'xdrop_final' : '-Z',
'xdrop_extension' : '-y',
'model_threshold' : '-h',
'required_start' : '-S',
'required_end' : '-H',
'program' : '-p',
'database' : '-d',
'infile' : '-i',
'filter' : '-F',
'believe_query' : '-J',
'nprocessors' : '-a',
'html' : '-T',
'descriptions' : '-v',
'alignments' : '-b',
'align_view' : '-m',
'show_gi' : '-I',
'seqalign_file' : '-O',
'align_outfile' : '-o',
'checkpoint_outfile' : '-C',
'restart_infile' : '-R',
'hit_infile' : '-k',
'matrix_outfile' : '-Q',
'align_infile' : '-B',
}
from Applications import BlastpgpCommandline
cline = BlastpgpCommandline(blastcmd)
cline.set_parameter(att2param['database'], database)
cline.set_parameter(att2param['infile'], infile)
cline.set_parameter(att2param['align_view'], str(align_view))
for key, value in keywds.iteritems():
cline.set_parameter(att2param[key], str(value))
return _invoke_blast(cline)
def rpsblast(blastcmd, database, infile, align_view="7", **keywds):
"""Execute and retrieve data from standalone RPS-BLAST as handles (OBSOLETE).
NOTE - This function is obsolete, you are encouraged to the command
line wrapper Bio.Blast.Applications.RpsBlastCommandline instead.
Execute and retrieve data from standalone RPS-BLAST. blastcmd is the
command used to launch the 'rpsblast' executable. database is the path
to the database to search against. infile is the path to the file
containing the sequence to search with.
The return values are two handles, for standard output and standard error.
You may pass more parameters to **keywds to change the behavior of
the search. Otherwise, optional values will be chosen by rpsblast.
Please note that this function will give XML output by default, by
setting align_view to seven (i.e. command line option -m 7).
You should use the NCBIXML.parse() function to read the resulting output.
This is because NCBIStandalone.BlastParser() does not understand the
plain text output format from rpsblast.
WARNING - The following text and associated parameter handling has not
received extensive testing. Please report any errors we might have made...
Algorithm/Scoring
gapped Whether to do a gapped alignment. T/F
multihit 0 for multiple hit (default), 1 for single hit
expectation Expectation value cutoff.
range_restriction Range restriction on query sequence (Format: start,stop) blastp only
0 in 'start' refers to the beginning of the sequence
0 in 'stop' refers to the end of the sequence
Default = 0,0
xdrop Dropoff value (bits) for gapped alignments.
xdrop_final X dropoff for final gapped alignment (in bits).
xdrop_extension Dropoff for blast extensions (in bits).
search_length Effective length of search space.
nbits_gapping Number of bits to trigger gapping.
protein Query sequence is protein. T/F
db_length Effective database length.
Processing
filter Filter query sequence for low complexity? T/F
case_filter Use lower case filtering of FASTA sequence T/F, default F
believe_query Believe the query defline. T/F
nprocessors Number of processors to use.
logfile Name of log file to use, default rpsblast.log
Formatting
html Produce HTML output? T/F
descriptions Number of one-line descriptions.
alignments Number of alignments.
align_view Alignment view. Integer 0-11,
passed as a string or integer.
show_gi Show GI's in deflines? T/F
seqalign_file seqalign file to output.
align_outfile Output file for alignment. Filename to write to, if
ommitted standard output is used (which you can access
from the returned handles).
"""
import warnings
warnings.warn("This function is obsolete, you are encouraged to the command line wrapper Bio.Blast.Applications.BlastrpsCommandline instead.", PendingDeprecationWarning)
_security_check_parameters(keywds)
att2param = {
'multihit' : '-P',
'gapped' : '-g',
'expectation' : '-e',
'range_restriction' : '-L',
'xdrop' : '-X',
'xdrop_final' : '-Z',
'xdrop_extension' : '-y',
'search_length' : '-Y',
'nbits_gapping' : '-N',
'protein' : '-p',
'db_length' : '-z',
'database' : '-d',
'infile' : '-i',
'filter' : '-F',
'case_filter' : '-U',
'believe_query' : '-J',
'nprocessors' : '-a',
'logfile' : '-l',
'html' : '-T',
'descriptions' : '-v',
'alignments' : '-b',
'align_view' : '-m',
'show_gi' : '-I',
'seqalign_file' : '-O',
'align_outfile' : '-o',
}
from Applications import RpsBlastCommandline
cline = RpsBlastCommandline(blastcmd)
cline.set_parameter(att2param['database'], database)
cline.set_parameter(att2param['infile'], infile)
cline.set_parameter(att2param['align_view'], str(align_view))
for key, value in keywds.iteritems():
cline.set_parameter(att2param[key], str(value))
return _invoke_blast(cline)
def _re_search(regex, line, error_msg):
m = re.search(regex, line)
if not m:
raise ValueError(error_msg)
return m.groups()
def _get_cols(line, cols_to_get, ncols=None, expected={}):
cols = line.split()
# Check to make sure number of columns is correct
if ncols is not None and len(cols) != ncols:
raise ValueError("I expected %d columns (got %d) in line\n%s" \
% (ncols, len(cols), line))
# Check to make sure columns contain the correct data
for k in expected:
if cols[k] != expected[k]:
raise ValueError("I expected '%s' in column %d in line\n%s" \
% (expected[k], k, line))
# Construct the answer tuple
results = []
for c in cols_to_get:
results.append(cols[c])
return tuple(results)
def _safe_int(str):
try:
return int(str)
except ValueError:
# Something went wrong. Try to clean up the string.
# Remove all commas from the string
str = str.replace(',', '')
# try again after removing commas.
# Note int() will return a long rather than overflow
try:
return int(str)
except ValueError:
pass
# Call float to handle things like "54.3", note could lose precision, e.g.
# >>> int("5399354557888517312")
# 5399354557888517312
# >>> int(float("5399354557888517312"))
# 5399354557888517120
return int(float(str))
def _safe_float(str):
# Thomas Rosleff Soerensen (rosleff@mpiz-koeln.mpg.de) noted that
# float('e-172') does not produce an error on his platform. Thus,
# we need to check the string for this condition.
# Sometimes BLAST leaves of the '1' in front of an exponent.
if str and str[0] in ['E', 'e']:
str = '1' + str
try:
return float(str)
except ValueError:
# Remove all commas from the string
str = str.replace(',', '')
# try again.
return float(str)
def _invoke_blast(cline):
"""Start BLAST and returns handles for stdout and stderr (PRIVATE).
Expects a command line wrapper object from Bio.Blast.Applications
"""
import subprocess, sys
blast_cmd = cline.program_name
if not os.path.exists(blast_cmd):
raise ValueError("BLAST executable does not exist at %s" % blast_cmd)
#We don't need to supply any piped input, but we setup the
#standard input pipe anyway as a work around for a python
#bug if this is called from a Windows GUI program. For
#details, see http://bugs.python.org/issue1124861
blast_process = subprocess.Popen(str(cline),
stdin=subprocess.PIPE,
stdout=subprocess.PIPE,
stderr=subprocess.PIPE,
universal_newlines=True,
shell=(sys.platform!="win32"))
blast_process.stdin.close()
return blast_process.stdout, blast_process.stderr
def _security_check_parameters(param_dict):
"""Look for any attempt to insert a command into a parameter.
e.g. blastall(..., matrix='IDENTITY -F 0; rm -rf /etc/passwd')
Looks for ";" or "&&" in the strings (Unix and Windows syntax
for appending a command line), or ">", "<" or "|" (redirection)
and if any are found raises an exception.
"""
for key, value in param_dict.iteritems():
str_value = str(value) # Could easily be an int or a float
for bad_str in [";", "&&", ">", "<", "|"]:
if bad_str in str_value:
raise ValueError("Rejecting suspicious argument for %s" % key)
class _BlastErrorConsumer(_BlastConsumer):
def __init__(self):
_BlastConsumer.__init__(self)
def noevent(self, line):
if line.find("Query must be at least wordsize") != -1:
raise ShortQueryBlastError("Query must be at least wordsize")
# Now pass the line back up to the superclass.
method = getattr(_BlastConsumer, 'noevent',
_BlastConsumer.__getattr__(self, 'noevent'))
method(line)
class BlastErrorParser(AbstractParser):
"""Attempt to catch and diagnose BLAST errors while parsing.
This utilizes the BlastParser module but adds an additional layer
of complexity on top of it by attempting to diagnose ValueErrors
that may actually indicate problems during BLAST parsing.
Current BLAST problems this detects are:
o LowQualityBlastError - When BLASTing really low quality sequences
(ie. some GenBank entries which are just short streches of a single
nucleotide), BLAST will report an error with the sequence and be
unable to search with this. This will lead to a badly formatted
BLAST report that the parsers choke on. The parser will convert the
ValueError to a LowQualityBlastError and attempt to provide useful
information.
"""
def __init__(self, bad_report_handle = None):
"""Initialize a parser that tries to catch BlastErrors.
Arguments:
o bad_report_handle - An optional argument specifying a handle
where bad reports should be sent. This would allow you to save
all of the bad reports to a file, for instance. If no handle
is specified, the bad reports will not be saved.
"""
self._bad_report_handle = bad_report_handle
#self._b_parser = BlastParser()
self._scanner = _Scanner()
self._consumer = _BlastErrorConsumer()
def parse(self, handle):
"""Parse a handle, attempting to diagnose errors.
"""
results = handle.read()
try:
self._scanner.feed(File.StringHandle(results), self._consumer)
except ValueError, msg:
# if we have a bad_report_file, save the info to it first
if self._bad_report_handle:
# send the info to the error handle
self._bad_report_handle.write(results)
# now we want to try and diagnose the error
self._diagnose_error(
File.StringHandle(results), self._consumer.data)
# if we got here we can't figure out the problem
# so we should pass along the syntax error we got
raise
return self._consumer.data
def _diagnose_error(self, handle, data_record):
"""Attempt to diagnose an error in the passed handle.
Arguments:
o handle - The handle potentially containing the error
o data_record - The data record partially created by the consumer.
"""
line = handle.readline()
while line:
# 'Searchingdone' instead of 'Searching......done' seems
# to indicate a failure to perform the BLAST due to
# low quality sequence
if line.startswith('Searchingdone'):
raise LowQualityBlastError("Blast failure occured on query: ",
data_record.query)
line = handle.readline()
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