/usr/share/pyshared/Bio/Sequencing/Phd.py is in python-biopython 1.58-1.
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1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 | # Copyright 2004 by Cymon J. Cox and Frank Kauff. All rights reserved.
# Copyright 2008 by Michiel de Hoon. All rights reserved.
# Revisions copyright 2009 by Cymon J. Cox. All rights reserved.
# Revisions copyright 2009 by Peter Cock. All rights reserved.
#
# This code is part of the Biopython distribution and governed by its
# license. Please see the LICENSE file that should have been included
# as part of this package.
"""
Parser for PHD files output by PHRED and used by PHRAP and CONSED.
This module can be used used directly which will return Record objects
which should contain all the original data in the file.
Alternatively, using Bio.SeqIO with the "phd" format will call this module
internally. This will give SeqRecord objects for each contig sequence.
"""
from Bio import Seq
from Bio.Alphabet import generic_dna
CKEYWORDS=['CHROMAT_FILE','ABI_THUMBPRINT','PHRED_VERSION','CALL_METHOD',\
'QUALITY_LEVELS','TIME','TRACE_ARRAY_MIN_INDEX','TRACE_ARRAY_MAX_INDEX',\
'TRIM','TRACE_PEAK_AREA_RATIO','CHEM','DYE']
class Record(object):
"""Hold information from a PHD file."""
def __init__(self):
self.file_name = ''
self.comments = {}
for kw in CKEYWORDS:
self.comments[kw.lower()] = None
self.sites = []
self.seq = ''
self.seq_trimmed = ''
def read(handle):
"""Reads the next PHD record from the file, returning it as a Record object.
This function reads PHD file data line by line from the handle,
and returns a single Record object.
"""
for line in handle:
if line.startswith("BEGIN_SEQUENCE"):
record = Record()
record.file_name = line[15:].rstrip()
break
else:
return # No record found
for line in handle:
if line.startswith("BEGIN_COMMENT"):
break
else:
raise ValueError("Failed to find BEGIN_COMMENT line")
for line in handle:
line = line.strip()
if not line:
continue
if line=="END_COMMENT":
break
keyword, value = line.split(":", 1)
keyword = keyword.lower()
value = value.strip()
if keyword in ('chromat_file',
'phred_version',
'call_method',
'chem',
'dye',
'time',
'basecaller_version',
'trace_processor_version'):
record.comments[keyword] = value
elif keyword in ('abi_thumbprint',
'quality_levels',
'trace_array_min_index',
'trace_array_max_index'):
record.comments[keyword] = int(value)
elif keyword=='trace_peak_area_ratio':
record.comments[keyword] = float(value)
elif keyword=='trim':
first, last, prob = value.split()
record.comments[keyword] = (int(first), int(last), float(prob))
else:
raise ValueError("Failed to find END_COMMENT line")
for line in handle:
if line.startswith('BEGIN_DNA'):
break
else:
raise ValueError("Failed to find BEGIN_DNA line")
for line in handle:
if line.startswith('END_DNA'):
break
else:
# Line is: "site quality peak_location"
# Peak location is optional according to
# David Gordon (the Consed author)
parts = line.split()
if len(parts) in [2,3]:
record.sites.append(tuple(parts))
else:
raise ValueError("DNA line must contain a base and quality "
"score, and optionally a peak location.")
for line in handle:
if line.startswith("END_SEQUENCE"):
break
else:
raise ValueError("Failed to find END_SEQUENCE line")
record.seq = Seq.Seq(''.join([n[0] for n in record.sites]), generic_dna)
if record.comments['trim'] is not None:
first, last = record.comments['trim'][:2]
record.seq_trimmed = record.seq[first:last]
return record
def parse(handle):
"""Iterates over a file returning multiple PHD records.
The data is read line by line from the handle. The handle can be a list
of lines, an open file, or similar; the only requirement is that we can
iterate over the handle to retrieve lines from it.
Typical usage:
records = parse(handle)
for record in records:
# do something with the record object
"""
while True:
record = read(handle)
if not record:
return
yield record
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