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XX
AC J01636; J01637; K01483; K01793;
XX
DT 30-NOV-1990 (Rel. 26, Created)
DT 09-SEP-2004 (Rel. 81, Last updated, Version 8)
XX
DE E.coli lactose operon with lacI, lacZ, lacY and lacA genes.
XX
KW acetyltransferase; beta-D-galactosidase; galactosidase; lac operon;
KW lac repressor protein; lacA gene; lacI gene; lactose permease; lacY gene;
KW lacZ gene; mutagenesis; palindrome; promoter region;
KW thiogalactoside acetyltransferase.
XX
OS Escherichia coli
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
XX
RN [1]
RP 1243-1266
RX DOI; 10.1073/pnas.70.12.3581.
RX PUBMED; 4587255.
RA Gilbert W., Maxam A.;
RT "The nucleotide sequence of the lac operator";
RL Proc. Natl. Acad. Sci. U.S.A. 70(12):3581-3584(1973).
XX
RN [2]
RP 1246-1308
RX DOI; 10.1073/pnas.70.12.3585.
RX PUBMED; 4587256.
RA Maizels N.M.;
RT "The nucleotide sequence of the lactose messenger ribonucleic acid
RT transcribed from the UV5 promoter mutant of Escherichia coli";
RL Proc. Natl. Acad. Sci. U.S.A. 70(12):3585-3589(1973).
XX
RN [3]
RX PUBMED; 4598642.
RA Gilbert W., Maizels N., Maxam A.;
RT "Sequences of controlling regions of the lactose operon";
RL Cold Spring Harb. Symp. Quant. Biol. 38:845-855(1974).
XX
RN [4]
RA Gilbert W., Gralla J., Majors A.J., Maxam A.;
RT "Lactose operator sequences and the action of lac repressor";
RL (in) Sund H., Blauer G. (Eds.);
RL PROTEIN-LIGAND INTERACTIONS:193-207;
RL Walter de Gruyter, New York (1975)
XX
RN [5]
RP 1146-1282
RX DOI; 10.1126/science.1088926.
RX PUBMED; 1088926.
RA Dickson R.C., Abelson J.N., Barnes W.M., Reznikoff W.S.;
RT "Genetic regulation: the Lac control region";
RL Science 187(4171):27-35(1975).
XX
RN [6]
RP 1227-1271
RA Gilbert W., Maxam A., Mirzabekov A.;
RT "Contacts between the lac repressor and DNA revealed by methylation";
RL (in) Kjeldgaard N.C., Maaloe O. (Eds.);
RL CONTROL OF RIBOSOME SYNTHESIS:138-143;
RL Academic Press, New York (1976)
XX
RN [7]
RX DOI; 10.1038/260360a0.
RX PUBMED; 768781.
RA Marians K.J., Wu R.;
RT "Structure of the lactose operator";
RL Nature 260(5549):360-363(1976).
XX
RN [8]
RP 1242-1268
RX DOI; 10.1038/263748a0.
RX PUBMED; 1069185.
RA Heyneker H.L., Shine J., Goodman H.M., Boyer H.W., Rosenberg J.,
RA Dickerson R.E., Narang S.A., Itakura K., Lin S., Riggs A.D.;
RT "Synthetic lac operator DNA is functional in vivo";
RL Nature 263(5580):748-752(1976).
XX
RN [9]
RX DOI; 10.1016/S0022-2836(77)80132-0.
RX PUBMED; 323498.
RA Dickson R.C., Abelson J.N., Johnson P., Reznikoff W.S., Barnes W.M.;
RT "Nucleotide sequence changes produced by mutations in the lac promoter of
RT Escherichia coli";
RL J. Mol. Biol. 111(1):65-75(1977).
XX
RN [10]
RP 51-264
RX DOI; 10.1073/pnas.74.10.4163.
RX PUBMED; 337294.
RA Steege D.A.;
RT "5'-Terminal nucleotide sequence of Escherichia coli lactose repressor
RT mRNA: features of translational initiation and reinitiation sites";
RL Proc. Natl. Acad. Sci. U.S.A. 74(10):4163-4167(1977).
XX
RN [11]
RP 1-81
RX DOI; 10.1038/274762a0.
RX PUBMED; 355890.
RA Calos M.P.;
RT "DNA sequence for a low-level promoter of the lac repressor gene and an
RT 'up' promoter mutation";
RL Nature 274(5673):762-765(1978).
XX
RN [12]
RP 49-1161
RX DOI; 10.1038/274765a0.
RX PUBMED; 355891.
RA Farabaugh P.J.;
RT "Sequence of the lacI gene";
RL Nature 274(5673):765-769(1978).
XX
RN [13]
RX DOI; 10.1038/274770a0.
RX PUBMED; 355892.
RA Miller J.H., Coulondre C., Farabaugh P.J.;
RT "Correlation of nonsense sites in the lacI gene with specific codons in the
RT nucleotide sequence";
RL Nature 274(5673):770-775(1978).
XX
RN [14]
RX DOI; 10.1007/BF00267233.
RX PUBMED; 6770231.
RA Calos M.P., Miller J.H.;
RT "DNA sequence alteration resulting from a mutation impairing promoter
RT function in the lac repressor gene";
RL Mol. Gen. Genet. 178(1):225-227(1980).
XX
RN [15]
RP 4306-5804
RX DOI; 10.1038/283541a0.
RX PUBMED; 6444453.
RA Buechel D.E., Gronenborn B., Mueller-Hill B.;
RT "Sequence of the lactose permease gene";
RL Nature 283(5747):541-545(1980).
XX
RN [16]
RX PUBMED; 6271472.
RA Miller J.H., Calos M.P., Galas D.J.;
RT "Genetic and sequencing studies of the specificity of transposition into
RT the lac region of E. coli";
RL Cold Spring Harb. Symp. Quant. Biol. 45:243-257(1981).
XX
RN [17]
RX DOI; 10.1016/0014-5793(81)81076-9.
RX PUBMED; 7023981.
RA Chenchick A., Beabealashvilli R.S., Mirzabekov A.;
RT "Topography of interaction of Escherichia coli RNA polymerase subunits with
RT lac UV5 promoter";
RL FEBS Lett. 128(1):46-50(1981).
XX
RN [18]
RX DOI; 10.1016/0378-1119(81)90038-X.
RX PUBMED; 7016667.
RA Betz J.L., Sadler J.R.;
RT "Variants of a cloned synthetic lactose operator. I. A palindromic dimer
RT lactose operator derived from one stand of the cloned 40-base pair
RT operator";
RL Gene 13(1):1-12(1981).
XX
RN [19]
RX DOI; 10.1016/0378-1119(81)90039-1.
RX PUBMED; 6263752.
RA Sadler J.R., Tecklenburg M.;
RT "Cloning and characterization of the natural lactose operator";
RL Gene 13(1):13-23(1981).
XX
RN [20]
RX DOI; 10.1016/0378-1119(81)90128-1.
RX PUBMED; 6271642.
RA Betz J.L., Sadler J.R.;
RT "Variants of a cloned synthetic lactose operator. II.
RT Chloramphenicol-resistant revertants retaining a lactose operator in the
RT CAT gene of plasmid pBR325";
RL Gene 15(2-3):187-200(1981).
XX
RN [21]
RX DOI; 10.1007/BF00268783.
RX PUBMED; 7038381.
RA Calos M.P., Miller J.H.;
RT "The DNA sequence change resulting from the IQ1 mutation, which greatly
RT increases promoter strength";
RL Mol. Gen. Genet. 183(3):559-560(1981).
XX
RN [22]
RX DOI; 10.1073/pnas.78.12.7652.
RX PUBMED; 6278484.
RA Mieschendahl M., Buechel D.E., Bocklage H., Mueller-Hill B.;
RT "Mutations in the lacY gene of Escherichia coli define functional
RT organization of lactose permease";
RL Proc. Natl. Acad. Sci. U.S.A. 78(12):7652-7656(1981).
XX
RN [23]
RX DOI; 10.1016/0378-1119(82)90042-7.
RX PUBMED; 6299890.
RA Russell D.R., Bennett G.N.;
RT "Construction and analysis of in vivo activity of E. coli promoter hybrids
RT and promoter mutants that alter the -35 to -10 spacing";
RL Gene 20(2):231-243(1982).
XX
RN [24]
RX PUBMED; 6288696.
RA Horowitz H., Platt T.;
RT "A termination site for LacI transcription is between the CAP site and the
RT lac promoter";
RL J Biol Chem 257(19):11740-11746(1982).
XX
RN [25]
RX PUBMED; 6290487.
RA Klein R.D., Wells R.D.;
RT "Effects of neighboring DNA homopolymers on the biochemical and physical
RT properties of the Escherichia coli lactose promoter. I. Cloning and
RT characterization studies";
RL J Biol Chem 257(21):12954-12961(1982).
XX
RN [26]
RP 1183-1291
RX DOI; 10.1073/pnas.79.5.1408.
RX PUBMED; 7041119.
RA Weiher H., Schaller H.;
RT "Segment-specific mutagenesis: extensive mutagenesis of a lac
RT promoter/operator element";
RL Proc. Natl. Acad. Sci. U.S.A. 79(5):1408-1412(1982).
XX
RN [27]
RX PUBMED; 6305557.
RA Van Dyke M.W., Dervan P.B.;
RT "Footprinting with MPE-Fe(II). Complementary-strand analyses of distamycin-
RT and actinomycin-binding sites on heterogeneous DNA";
RL Cold Spring Harb. Symp. Quant. Biol. 47:347-353(1983).
XX
RN [28]
RP 1287-4364
RX PUBMED; 6313347.
RA Kalnins A., Otto K., Ruether U., Mueller-Hill B.;
RT "Sequence of the lacZ gene of Escherichia coli";
RL EMBO J. 2(4):593-597(1983).
XX
RN [29]
RX PUBMED; 6309841.
RA Cone K.C., Sellitti M.A., Steege D.A.;
RT "Lac repressor mRNA transcription terminates in vivo in the lac control
RT region";
RL J Biol Chem 258(18):11296-11304(1983).
XX
RN [30]
RX DOI; 10.1073/pnas.80.22.6785.
RX PUBMED; 6316325.
RA Sadler J.R., Sasmor H., Betz J.L.;
RT "A perfectly symmetric lac operator binds the lac repressor very tightly";
RL Proc. Natl. Acad. Sci. U.S.A. 80(22):6785-6789(1983).
XX
RN [31]
RX DOI; 10.1073/pnas.81.2.512.
RX PUBMED; 6582506.
RA Glickman B.W., Ripley L.S.;
RT "Structural intermediates of deletion mutagenesis: a role for palindromic
RT DNA";
RL Proc. Natl. Acad. Sci. U.S.A. 81(2):512-516(1984).
XX
RN [32]
RX DOI; 10.1021/bi00332a019.
RX PUBMED; 3896305.
RA Spassky A., Kirkegaard K., Buc H.;
RT "Changes in the DNA structure of the lac UV5 promoter during formation of
RT an open complex with Escherichia coli RNA polymerase";
RL Biochemistry 24(11):2723-2731(1985).
XX
RN [33]
RX DOI; 10.1016/0092-8674(85)90175-8.
RX PUBMED; 2416465.
RA Straney D.C., Crothers D.M.;
RT "Intermediates in transcription initiation from the E. coli lac UV5
RT promoter";
RL Cell 43(2 Pt 1):449-459(1985).
XX
RN [34]
RX DOI; 10.1016/0378-1119(85)90267-7.
RX PUBMED; 3932130.
RA Looman A.C., de Gruyter M., Vogelaar A., van Knippenberg P.H.;
RT "Effects of heterologous ribosomal binding sites on the transcription and
RT translation of the lacZ gene of Escherichia coli";
RL Gene 37(1-3):145-154(1985).
XX
RN [35]
RX PUBMED; 2999082.
RA Mandecki W., Goldman R.A., Powell B.S., Caruthers M.H.;
RT "lac Up-promoter mutants with increased homology to the consensus promoter
RT sequence";
RL J. Bacteriol. 164(3):1353-1355(1985).
XX
RN [36]
RX DOI; 10.1016/0022-2836(85)90427-9.
RX PUBMED; 2987506.
RA Malamy M.H., Rahaim P.T., Hoffman C.S., Baghdoyan D., O'Connor M.B.,
RA Miller J.F.;
RT "A frameshift mutation at the junction of an IS1 insertion within lacZ
RT restores beta-galactosidase activity via formation of an active lacZ-IS1
RT fusion protein";
RL J. Mol. Biol. 181(4):551-555(1985).
XX
RN [37]
RP 5646-7477
RX DOI; 10.1073/pnas.82.19.6414.
RX PUBMED; 3901000.
RA Hediger M.A., Johnson D.F., Nierlich D.P., Zabin I.;
RT "DNA sequence of the lactose operon: the lacA gene and the transcriptional
RT termination region";
RL Proc. Natl. Acad. Sci. U.S.A. 82(19):6414-6418(1985).
XX
CC [3] sites; UV5 mRNA transcripts and operator mutants. [(in)
CC Sund,H. and Blauer,G. (eds.);Protein-Ligand Interactions:
CC 193-207;Walter de] sites; operator mutational analysis. [7]
CC sites; S1 and mung bean nuclease action on operator DNA. [9]
CC sites; class I, II and III promoter mutant analysis. [13] sites;
CC lacI mutant analysis.
CC [16] sites; Tn5, Tn9 and Tn10 insertion sites in lac region. [14]
CC sites; lacI promoter mutation UJ177.
CC [18] sites; palindromic dimer operator;.
CC [19] sites; natural operator sequence.
CC [20] sites; operator mutational analysis.
CC [21] sites; lacI-Q deletion.
CC [17] sites; RNA polymerase UV5 promoter interaction. [22] sites;
CC lacY mutational analysis.
CC [24] sites; lacI-promoted transcription termination. [25] sites;
CC wt and UV5 promoter sequence studies. [23] sites; UV5 promoter
CC mutational analysis.
CC [30] sites; perfectly symmetric operator sequence. [29] sites;
CC lacI mRNA termination site.
CC [27] sites; distamycin and actinomycin binding to promoter. [31]
CC sites; lacI deletion studies.
CC [35] sites; promoter mutational studies.
CC [33] sites; DNAase I studies with promoter sequence. [34] sites;
CC ribosomal binding and translation initiation for lacZ. [36] sites;
CC insertion sequence IS1 integration in lacZ;. [32] sites; DNAase I
CC studies with promoter.
CC [1] first reports a 27 bp operator(sites 1240-1266) with two-fold
CC symmetries; the operator has also been defined to be bases
CC 1246-1266 or bases 1239-1273 [8]. [(in) Kjeldgaard,N.C. and Maaloe,
CC O.(eds);Control of ribosome synthesis: 138-143;A] explores the
CC ability of lac
CC repressor protein to affect methylation of operator DNA. [8]
CC argues that DNA on both sides of the 21 bp operator (bases
CC 1246-1266) affects repressor binding but that the sequences of this
CC DNA are probably not critical. [5] gives a larger sequence known as
CC the promoter-operator region for the wild-type, whereas [2] and
CC [26] give portions of this region for the mutant strain UV5. Within
CC the promoter region, bases 1162-1199 are identified as the
CC catabolite gene activator protein binding site (cap) and bases
CC 1200-1245 are the RNA polymerase interaction site. [10] reports a
CC sequence for the 5'end of the lacI (repressor) gene and discusses
CC restart in mutant strains. [11] presents a sequence for the lacI
CC promoter region and identifies an I-Q mutation which enhances lacI
CC transcription approximately ten-fold. [12] gives a complete
CC sequence
CC for lacI which agrees with the known lac repressor sequence. [26]
CC examines the promoter-operator region in the UV5 strain (lac109)
CC and studies 23 mutant derivatives of this sequence. This sequence
CC agrees with known protein sequences for the lacZ, lacY and lacA
CC enzymes. [15] notes that the fMet codon is not present
CC for lacA and suggests that the 'ttg' codon (5727-5729), which
CC immediately precedes the mature N-terminal asparagine codon, is the
CC start codon. The cds for lacZ, lacY and lacA are included on a
CC single mRNA transcript.
CC Complete source information:
CC Escherichia coli DNA [1],[(in) Kjeldgaard,N.C. and Maaloe,O.(eds);
CC Control of ribosome synthesis: 138-143;A],[8],[12],[26]; mRNA [2],
CC [5],[10]; clone
CC lambda-h80dlac DNA [11],[15]; clone puk217 [28]; pgm8 [37].
XX
FH Key Location/Qualifiers
FH
FT source 1..7477
FT /organism="Escherichia coli"
FT /mol_type="genomic DNA"
FT /db_xref="taxon:562"
FT variation 16
FT /note="c in wild-type; t in 'up' promoter mutant I-Q [11]"
FT mRNA 51..1230
FT /note="lacI (repressor) mRNA; preferred in vivo 3' end
FT [12],[29]"
FT CDS 79..1161
FT /codon_start=1
FT /transl_table=11
FT /gene="lacI"
FT /note="lac repressor protein (gtg start codon)"
FT /db_xref="GOA:P03023"
FT /db_xref="InterPro:IPR000843"
FT /db_xref="InterPro:IPR010982"
FT /db_xref="PDB:1CJG"
FT /db_xref="PDB:1EFA"
FT /db_xref="PDB:1JWL"
FT /db_xref="PDB:1JYE"
FT /db_xref="PDB:1JYF"
FT /db_xref="PDB:1L1M"
FT /db_xref="PDB:1LBG"
FT /db_xref="PDB:1LBH"
FT /db_xref="PDB:1LBI"
FT /db_xref="PDB:1LCC"
FT /db_xref="PDB:1LCD"
FT /db_xref="PDB:1LQC"
FT /db_xref="PDB:1LTP"
FT /db_xref="PDB:1OSL"
FT /db_xref="PDB:1TLF"
FT /db_xref="PDB:1Z04"
FT /db_xref="PDB:2BJC"
FT /db_xref="PDB:2KEI"
FT /db_xref="PDB:2KEJ"
FT /db_xref="PDB:2KEK"
FT /db_xref="PDB:2P9H"
FT /db_xref="PDB:2PAF"
FT /db_xref="PDB:2PE5"
FT /db_xref="PDB:3EDC"
FT /db_xref="UniProtKB/Swiss-Prot:P03023"
FT /protein_id="AAA24052.1"
FT /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
FT NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
FT EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
FT EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
FT MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
FT YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
FT ALADSLMQLARQVSRLESGQ"
FT misc_signal 1162..1199
FT /note="cap protein binding site"
FT variation 1183..1186
FT /note="ttag in wild-type; aatt in strain UV5 [26]"
FT variation 1209..1211
FT /note="gct in wild-type; gt in mutant l305 [5]"
FT variation 1212
FT /note="t in wild-type; a in mutant l241 [5]"
FT variation 1230
FT /note="c in wild-type; a in mutant p-r-1a [5]"
FT variation 1237..1238
FT /note="gt in wild-type; aa in strain UV5 [26]"
FT variation 1242..1245
FT /note="gtgg in wild-type; ttca in synthetic operator [8]"
FT misc_signal 1246..1266
FT /note="lac repressor protein binding site"
FT mRNA 1246..>4358
FT /note="lacZ mRNA [2],[5]"
FT variation 1267..1268
FT /note="tc in wild-type; tg in synthetic operator [8]"
FT variation 1282..1291
FT /note="ctatgaccat in wild-type; gatccggcca in strain UV5
FT [26]"
FT CDS 1284..4358
FT /codon_start=1
FT /transl_table=11
FT /gene="lacZ"
FT /note="beta-d-galactosidase"
FT /db_xref="GOA:P00722"
FT /db_xref="InterPro:IPR004199"
FT /db_xref="InterPro:IPR006101"
FT /db_xref="InterPro:IPR006102"
FT /db_xref="InterPro:IPR006103"
FT /db_xref="InterPro:IPR006104"
FT /db_xref="InterPro:IPR008979"
FT /db_xref="InterPro:IPR011013"
FT /db_xref="InterPro:IPR013781"
FT /db_xref="InterPro:IPR013812"
FT /db_xref="InterPro:IPR014718"
FT /db_xref="InterPro:IPR017853"
FT /db_xref="InterPro:IPR023230"
FT /db_xref="InterPro:IPR023232"
FT /db_xref="InterPro:IPR023933"
FT /db_xref="PDB:1BGL"
FT /db_xref="PDB:1BGM"
FT /db_xref="PDB:1DP0"
FT /db_xref="PDB:1F49"
FT /db_xref="PDB:1F4A"
FT /db_xref="PDB:1F4H"
FT /db_xref="PDB:1GHO"
FT /db_xref="PDB:1HN1"
FT /db_xref="PDB:1JYN"
FT /db_xref="PDB:1JYV"
FT /db_xref="PDB:1JYW"
FT /db_xref="PDB:1JYX"
FT /db_xref="PDB:1JYY"
FT /db_xref="PDB:1JYZ"
FT /db_xref="PDB:1JZ0"
FT /db_xref="PDB:1JZ1"
FT /db_xref="PDB:1JZ2"
FT /db_xref="PDB:1JZ3"
FT /db_xref="PDB:1JZ4"
FT /db_xref="PDB:1JZ5"
FT /db_xref="PDB:1JZ6"
FT /db_xref="PDB:1JZ7"
FT /db_xref="PDB:1JZ8"
FT /db_xref="PDB:1PX3"
FT /db_xref="PDB:1PX4"
FT /db_xref="PDB:3CZJ"
FT /db_xref="PDB:3DYM"
FT /db_xref="PDB:3DYO"
FT /db_xref="PDB:3DYP"
FT /db_xref="PDB:3E1F"
FT /db_xref="PDB:3I3B"
FT /db_xref="PDB:3I3D"
FT /db_xref="PDB:3I3E"
FT /db_xref="PDB:3IAP"
FT /db_xref="PDB:3IAQ"
FT /db_xref="PDB:3MUY"
FT /db_xref="PDB:3MUZ"
FT /db_xref="PDB:3MV0"
FT /db_xref="PDB:3MV1"
FT /db_xref="PDB:3SEP"
FT /db_xref="PDB:3T08"
FT /db_xref="PDB:3T09"
FT /db_xref="PDB:3T0A"
FT /db_xref="PDB:3T0B"
FT /db_xref="PDB:3T0D"
FT /db_xref="PDB:3T2O"
FT /db_xref="PDB:3T2P"
FT /db_xref="PDB:3T2Q"
FT /db_xref="PDB:3VD3"
FT /db_xref="PDB:3VD4"
FT /db_xref="PDB:3VD5"
FT /db_xref="PDB:3VD7"
FT /db_xref="PDB:3VD9"
FT /db_xref="PDB:3VDA"
FT /db_xref="PDB:3VDB"
FT /db_xref="PDB:3VDC"
FT /db_xref="UniProtKB/Swiss-Prot:P00722"
FT /protein_id="AAA24053.1"
FT /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
FT DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
FT TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
FT QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
FT ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
FT ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
FT GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
FT YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
FT LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
FT KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
FT IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
FT TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
FT LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
FT NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
FT AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
FT EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
FT VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
FT FHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
FT CDS 4410..5663
FT /codon_start=1
FT /transl_table=11
FT /gene="lacY"
FT /note="lactose permease"
FT /db_xref="GOA:P02920"
FT /db_xref="InterPro:IPR000576"
FT /db_xref="InterPro:IPR016196"
FT /db_xref="InterPro:IPR018457"
FT /db_xref="InterPro:IPR020846"
FT /db_xref="InterPro:IPR022814"
FT /db_xref="PDB:1M2U"
FT /db_xref="PDB:1PV6"
FT /db_xref="PDB:1PV7"
FT /db_xref="PDB:2CFP"
FT /db_xref="PDB:2CFQ"
FT /db_xref="PDB:2V8N"
FT /db_xref="PDB:2Y5Y"
FT /db_xref="UniProtKB/Swiss-Prot:P02920"
FT /protein_id="AAA24054.1"
FT /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDT
FT GIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNIL
FT VGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFT
FT INNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKL
FT WFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPL
FT IINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQ
FT FEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFT
FT LSGPGPLSLLRRQVNEVA"
FT CDS 5727..6338
FT /codon_start=1
FT /transl_table=11
FT /gene="lacA"
FT /note="thiogalactoside acetyltransferase (ttg start codon)"
FT /db_xref="GOA:P07464"
FT /db_xref="InterPro:IPR001451"
FT /db_xref="InterPro:IPR011004"
FT /db_xref="InterPro:IPR018357"
FT /db_xref="InterPro:IPR024688"
FT /db_xref="PDB:1KQA"
FT /db_xref="PDB:1KRR"
FT /db_xref="PDB:1KRU"
FT /db_xref="PDB:1KRV"
FT /db_xref="UniProtKB/Swiss-Prot:P07464"
FT /protein_id="AAA24055.1"
FT /translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEVE
FT KRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNVLI
FT APNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGAGSI
FT VTKDIPPNVVAAGVPCRVIREINDRDKHYYFKDYKVESSV"
XX
SQ Sequence 7477 BP; 1739 A; 1991 C; 2004 G; 1743 T; 0 other;
gacaccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt 60
caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt 120
gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg 180
cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa 240
caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac 300
gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg 360
gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt 420
ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt 480
gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca 540
cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg 600
gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg 660
cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg 720
gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat 780
gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg 840
cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac 900
gataccgaag acagctcatg ttatatcccg ccgtcaacca ccatcaaaca ggattttcgc 960
ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag 1020
ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg 1080
caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 1140
cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc 1200
accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 1260
acaatttcac acaggaaaca gctatgacca tgattacgga ttcactggcc gtcgttttac 1320
aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc 1380
ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc 1440
gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa 1500
gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga 1560
tgcacggtta cgatgcgccc atctacacca acgtaaccta tcccattacg gtcaatccgc 1620
cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa 1680
gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc 1740
tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg 1800
acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgttgga 1860
gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg 1920
tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta 1980
atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg 2040
actacctacg ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg 2100
cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac 2160
gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg 2220
tggttgaact gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt 2280
tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc 2340
gaggcgttaa ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga 2400
tggtgcagga tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt 2460
atccgaacca tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg 2520
aagccaatat tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct 2580
ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga 2640
gtgtgatcat ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcgctgt 2700
atcgctggat caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg 2760
acaccacggc caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct 2820
tcccggctgt gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc 2880
cgctgatcct ttgcgaatac gcccacgcga tgggtaacag tcttggcggt ttcgctaaat 2940
actggcaggc gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg 3000
atcagtcgct gattaaatat gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt 3060
ttggcgatac gccgaacgat cgccagttct gtatgaacgg tctggtcttt gccgaccgca 3120
cgccgcatcc agcgctgacg gaagcaaaac accagcagca gtttttccag ttccgtttat 3180
ccgggcaaac catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc 3240
tgcactggat ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg cctctggatg 3300
tcgctccaca aggtaaacag ttgattgaac tgcctgaact accgcagccg gagagcgccg 3360
ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg 3420
ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg 3480
ccgcgtccca cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg 3540
gtaataagcg ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg 3600
ataaaaaaca actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg 3660
acattggcgt aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg 3720
cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg 3780
atgcggtgct gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca 3840
gccggaaaac ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag 3900
tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag 3960
cagagcgggt aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg 4020
ccgcctgttt tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc 4080
cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc 4140
gcggcgactt ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc 4200
atcgccatct gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg 4260
ggattggtgg cgacgactcc tggagcccgt cagtatcggc ggaattccag ctgagcgccg 4320
gtcgctacca ttaccagttg gtctggtgtc aaaaataata ataaccgggc aggccatgtc 4380
tgcccgtatt tcgcgtaagg aaatccatta tgtactattt aaaaaacaca aacttttgga 4440
tgttcggttt attctttttc ttttactttt ttatcatggg agcctacttc ccgtttttcc 4500
cgatttggct acatgacatc aaccatatca gcaaaagtga tacgggtatt atttttgccg 4560
ctatttctct gttctcgcta ttattccaac cgctgtttgg tctgctttct gacaaactcg 4620
ggctgcgcaa atacctgctg tggattatta ccggcatgtt agtgatgttt gcgccgttct 4680
ttatttttat cttcgggcca ctgttacaat acaacatttt agtaggatcg attgttggtg 4740
gtatttatct aggcttttgt tttaacgccg gtgcgccagc agtagaggca tttattgaga 4800
aagtcagccg tcgcagtaat ttcgaatttg gtcgcgcgcg gatgtttggc tgtgttggct 4860
gggcgctgtg tgcctcgatt gtcggcatca tgttcaccat caataatcag tttgttttct 4920
ggctgggctc tggctgtgca ctcatcctcg ccgttttact ctttttcgcc aaaacggatg 4980
cgccctcttc tgccacggtt gccaatgcgg taggtgccaa ccattcggca tttagcctta 5040
agctggcact ggaactgttc agacagccaa aactgtggtt tttgtcactg tatgttattg 5100
gcgtttcctg cacctacgat gtttttgacc aacagtttgc taatttcttt acttcgttct 5160
ttgctaccgg tgaacagggt acgcgggtat ttggctacgt aacgacaatg ggcgaattac 5220
ttaacgcctc gattatgttc tttgcgccac tgatcattaa tcgcatcggt gggaaaaacg 5280
ccctgctgct ggctggcact attatgtctg tacgtattat tggctcatcg ttcgccacct 5340
cagcgctgga agtggttatt ctgaaaacgc tgcatatgtt tgaagtaccg ttcctgctgg 5400
tgggctgctt taaatatatt accagccagt ttgaagtgcg tttttcagcg acgatttatc 5460
tggtctgttt ctgcttcttt aagcaactgg cgatgatttt tatgtctgta ctggcgggca 5520
atatgtatga aagcatcggt ttccagggcg cttatctggt gctgggtctg gtggcgctgg 5580
gcttcacctt aatttccgtg ttcacgctta gcggccccgg cccgctttcc ctgctgcgtc 5640
gtcaggtgaa tgaagtcgct taagcaatca atgtcggatg cggcgcgacg cttatccgac 5700
caacatatca taacggagtg atcgcattga acatgccaat gaccgaaaga ataagagcag 5760
gcaagctatt taccgatatg tgcgaaggct taccggaaaa aagacttcgt gggaaaacgt 5820
taatgtatga gtttaatcac tcgcatccat cagaagttga aaaaagagaa agcctgatta 5880
aagaaatgtt tgccacggta ggggaaaacg cctgggtaga accgcctgtc tatttctctt 5940
acggttccaa catccatata ggccgcaatt tttatgcaaa tttcaattta accattgtcg 6000
atgactacac ggtaacaatc ggtgataacg tactgattgc acccaacgtt actctttccg 6060
ttacgggaca ccctgtacac catgaattga gaaaaaacgg cgagatgtac tcttttccga 6120
taacgattgg caataacgtc tggatcggaa gtcatgtggt tattaatcca ggcgtcacca 6180
tcggggataa ttctgttatt ggcgcgggta gtatcgtcac aaaagacatt ccaccaaacg 6240
tcgtggcggc tggcgttcct tgtcgggtta ttcgcgaaat aaacgaccgg gataagcact 6300
attatttcaa agattataaa gttgaatcgt cagtttaaat tataaaaatt gcctgatacg 6360
ctgcgcttat caggcctaca agttcagcga tctacattag ccgcatccgg catgaacaaa 6420
gcgcaggaac aagcgtcgca tcatgcctct ttgacccaca gctgcggaaa acgtactggt 6480
gcaaaacgca gggttatgat catcagccca acgacgcaca gcgcatgaaa tgcccagtcc 6540
atcaggtaat tgccgctgat actacgcagc acgccagaaa accacggggc aagcccggcg 6600
atgataaaac cgattccctg cataaacgcc accagcttgc cagcaatagc cggttgcaca 6660
gagtgatcga gcgccagcag caaacagagc ggaaacgcgc cgcccagacc taacccacac 6720
accatcgccc acaataccgg caattgcatc ggcagccaga taaagccgca gaaccccacc 6780
agttgtaaca ccagcgccag cattaacagt ttgcgccgat cctgatggcg agccatagca 6840
ggcatcagca aagctcctgc ggcttgccca agcgtcatca atgccagtaa ggaaccgctg 6900
tactgcgcgc tggcaccaat ctcaatatag aaagcgggta accaggcaat caggctggcg 6960
taaccgccgt taatcagacc gaagtaaaca cccagcgtcc acgcgcgggg agtgaatacc 7020
acgcgaaccg gagtggttgt tgtcttgtgg gaagaggcga cctcgcgggc gctttgccac 7080
caccaggcaa agagcgcaac aacggcaggc agcgccacca ggcgagtgtt tgataccagg 7140
tttcgctatg ttgaactaac cagggcgtta tggcggcacc aagcccaccg ccgcccatca 7200
gagccgcgga ccacagcccc atcaccagtg gcgtgcgctg ctgaaaccgc cgtttaatca 7260
ccgaagcatc accgcctgaa tgatgccgat ccccacccca ccaagcagtg cgctgctaag 7320
cagcagcgca ctttgcgggt aaagctcacg catcaatgca ccgacggcaa tcagcaacag 7380
actgatggcg acactgcgac gttcgctgac atgctgatga agccagcttc cggccagcgc 7440
cagcccgccc atggtaacca ccggcagagc ggtcgac 7477
//
ID X51872; SV 1; linear; genomic DNA; STD; PRO; 1832 BP.
XX
AC X51872;
XX
DT 17-APR-1990 (Rel. 23, Created)
DT 05-JUL-1999 (Rel. 60, Last updated, Version 5)
XX
DE Escherichia coli lacA gene for thiogalactoside transacetylase
XX
KW lac operon; lacA gene; lacY gene; thiogalactoside transacetylase.
XX
OS Escherichia coli
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
XX
RN [1]
RC (1-1832)
RP 1-1832
RX DOI; 10.1073/pnas.82.19.6414.
RX PUBMED; 3901000.
RA Hediger M.A., Johnson D.F., Nierlich D.P., Zabin I.;
RT "DNA sequence of the lactose operon: the lacA gene and the transcriptional
RT termination region";
RL Proc. Natl. Acad. Sci. U.S.A. 82(19):6414-6418(1985).
XX
FH Key Location/Qualifiers
FH
FT source 1..1832
FT /organism="Escherichia coli"
FT /mol_type="genomic DNA"
FT /db_xref="taxon:562"
FT CDS <1..18
FT /codon_start=1
FT /transl_table=11
FT /product="lacY gene product"
FT /protein_id="CAA36161.1"
FT /translation="VNEVA"
FT CDS 82..693
FT /transl_table=11
FT /gene="lacA"
FT /product="thiogalactoside transacetylase"
FT /db_xref="GOA:P07464"
FT /db_xref="InterPro:IPR001451"
FT /db_xref="InterPro:IPR011004"
FT /db_xref="InterPro:IPR018357"
FT /db_xref="InterPro:IPR024688"
FT /db_xref="PDB:1KQA"
FT /db_xref="PDB:1KRR"
FT /db_xref="PDB:1KRU"
FT /db_xref="PDB:1KRV"
FT /db_xref="UniProtKB/Swiss-Prot:P07464"
FT /protein_id="CAA36162.1"
FT /translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEVE
FT KRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNVLI
FT APNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGAGSI
FT VTKDIPPNVVAAGVPCRVIREINDRDKHYYFKDYKVESSV"
XX
SQ Sequence 1832 BP; 519 A; 510 C; 450 G; 353 T; 0 other;
gtgaatgaag tcgcttaagc aatcaatgtc ggatgcggcg cgacgcttat ccgaccaaca 60
tatcataacg gagtgatcgc attgaacatg ccaatgaccg aaagaataag agcaggcaag 120
ctatttaccg atatgtgcga aggcttaccg gaaaaaagac ttcgtgggaa aacgttaatg 180
tatgagttta atcactcgca tccatcagaa gttgaaaaaa gagaaagcct gattaaagaa 240
atgtttgcca cggtagggga aaacgcctgg gtagaaccgc ctgtctattt ctcttacggt 300
tccaacatcc atataggccg caatttttat gcaaatttca atttaaccat tgtcgatgac 360
tacacggtaa caatcggtga taacgtactg attgcaccca acgttactct ttccgttacg 420
ggacaccctg tacaccatga attgagaaaa aacggcgaga tgtactcttt tccgataacg 480
attggcaata acgtctggat cggaagtcat gtggttatta atccaggcgt caccatcggg 540
gataattctg ttattggcgc gggtagtatc gtcacaaaag acattccacc aaacgtcgtg 600
gcggctggcg ttccttgtcg ggttattcgc gaaataaacg accgggataa gcactattat 660
ttcaaagatt ataaagttga atcgtcagtt taaattataa aaattgcctg atacgctgcg 720
cttatcaggc ctacaagttc agcgatctac attagccgca tccggcatga acaaagcgca 780
ggaacaagcg tcgcatcatg cctctttgac ccacagctgc ggaaaacgta ctggtgcaaa 840
acgcagggtt atgatcatca gcccaacgac gcacagcgca tgaaatgccc agtccatcag 900
gtaattgccg ctgatactac gcagcacgcc agaaaaccac ggggcaagcc cggcgatgat 960
aaaaccgatt ccctgcataa acgccaccag cttgccagca atagccggtt gcacagagtg 1020
atcgagcgcc agcagcaaac agagcggaaa cgcgccgccc agacctaacc cacacaccat 1080
cgcccacaat accggcaatt gcatcggcag ccagataaag ccgcagaacc ccaccagttg 1140
taacaccagc gccagcatta acagtttgcg ccgatcctga tggcgagcca tagcaggcat 1200
cagcaaagct cctgcggctt gcccaagcgt catcaatgcc agtaaggaac cgctgtactg 1260
cgcgctggca ccaatctcaa tatagaaagc gggtaaccag gcaatcaggc tggcgtaacc 1320
gccgttaatc agaccgaagt aaacacccag cgtccacgcg cggggagtga ataccacgcg 1380
aaccggagtg gttgttgtct tgtgggaaga ggcgacctcg cgggcgcttt gccaccacca 1440
ggcaaagagc gcaacaacgg caggcagcgc caccaggcga gtgtttgata ccaggtttcg 1500
ctatgttgaa ctaaccaggg cgttatggcg gcaccaagcc caccgccgcc catcagagcc 1560
gcggaccaca gccccatcac cagtggcgtg cgctgctgaa accgccgttt aatcaccgaa 1620
gcatcaccgc ctgaatgatg ccgatcccca ccccaccaag cagtgcgctg ctaagcagca 1680
gcgcactttg cgggtaaagc tcacgcatca atgcaccgac ggcaatcagc aacagactga 1740
tggcgacact gcgacgttcg ctgacatgct gatgaagcca gcttccggcc agcgccagcc 1800
cgcccatggt aaccaccggc agagcggtcg ac 1832
//
ID V00294; SV 1; linear; genomic DNA; STD; PRO; 1113 BP.
XX
AC V00294;
XX
DT 09-JUN-1982 (Rel. 01, Created)
DT 10-FEB-1999 (Rel. 58, Last updated, Version 2)
XX
DE E. coli laci gene (codes for the lac repressor).
XX
KW DNA binding protein; repressor.
XX
OS Escherichia coli
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
XX
RN [1]
RP 1-1113
RX DOI; 10.1038/274765a0.
RX PUBMED; 355891.
RA Farabaugh P.J.;
RT "Sequence of the lacI gene";
RL Nature 274(5673):765-769(1978).
XX
CC KST ECO.LACI
XX
FH Key Location/Qualifiers
FH
FT source 1..1113
FT /organism="Escherichia coli"
FT /mol_type="genomic DNA"
FT /db_xref="taxon:562"
FT CDS 31..1113
FT /transl_table=11
FT /note="reading frame"
FT /db_xref="GOA:P03023"
FT /db_xref="InterPro:IPR000843"
FT /db_xref="InterPro:IPR010982"
FT /db_xref="PDB:1CJG"
FT /db_xref="PDB:1EFA"
FT /db_xref="PDB:1JWL"
FT /db_xref="PDB:1JYE"
FT /db_xref="PDB:1JYF"
FT /db_xref="PDB:1L1M"
FT /db_xref="PDB:1LBG"
FT /db_xref="PDB:1LBH"
FT /db_xref="PDB:1LBI"
FT /db_xref="PDB:1LCC"
FT /db_xref="PDB:1LCD"
FT /db_xref="PDB:1LQC"
FT /db_xref="PDB:1LTP"
FT /db_xref="PDB:1OSL"
FT /db_xref="PDB:1TLF"
FT /db_xref="PDB:1Z04"
FT /db_xref="PDB:2BJC"
FT /db_xref="PDB:2KEI"
FT /db_xref="PDB:2KEJ"
FT /db_xref="PDB:2KEK"
FT /db_xref="PDB:2P9H"
FT /db_xref="PDB:2PAF"
FT /db_xref="PDB:2PE5"
FT /db_xref="PDB:3EDC"
FT /db_xref="UniProtKB/Swiss-Prot:P03023"
FT /protein_id="CAA23569.1"
FT /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
FT NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
FT EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
FT EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
FT MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
FT YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
FT ALADSLMQLARQVSRLESGQ"
XX
SQ Sequence 1113 BP; 249 A; 304 C; 322 G; 238 T; 0 other;
ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca 60
gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt 120
tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac 180
cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt 240
ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg 300
ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg 360
gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac 420
caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc 480
tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc 540
gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt 600
tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt 660
cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg 720
caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg 780
ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta 840
gtgggatacg acgataccga agacagctca tgttatatcc cgccgtcaac caccatcaaa 900
caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc 960
caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg 1020
gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 1080
cgacaggttt cccgactgga aagcgggcag tga 1113
//
ID V00295; SV 1; linear; genomic DNA; STD; PRO; 1500 BP.
XX
AC V00295;
XX
DT 09-JUN-1982 (Rel. 01, Created)
DT 07-JUL-1995 (Rel. 44, Last updated, Version 4)
XX
DE E. coli lacY gene (codes for lactose permease).
XX
KW membrane protein.
XX
OS Escherichia coli
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
XX
RN [1]
RP 1-1500
RX DOI; 10.1038/283541a0.
RX PUBMED; 6444453.
RA Buechel D.E., Gronenborn B., Mueller-Hill B.;
RT "Sequence of the lactose permease gene";
RL Nature 283(5747):541-545(1980).
XX
CC lacZ is a beta-galactosidase and lacA is transacetylase.
CC KST ECO.LACY
XX
FH Key Location/Qualifiers
FH
FT source 1..1500
FT /organism="Escherichia coli"
FT /mol_type="genomic DNA"
FT /db_xref="taxon:562"
FT CDS <1..54
FT /codon_start=1
FT /transl_table=11
FT /note="reading frame (lacZ)"
FT /db_xref="GOA:P00722"
FT /db_xref="InterPro:IPR004199"
FT /db_xref="InterPro:IPR006101"
FT /db_xref="InterPro:IPR006102"
FT /db_xref="InterPro:IPR006103"
FT /db_xref="InterPro:IPR006104"
FT /db_xref="InterPro:IPR008979"
FT /db_xref="InterPro:IPR011013"
FT /db_xref="InterPro:IPR013781"
FT /db_xref="InterPro:IPR013812"
FT /db_xref="InterPro:IPR014718"
FT /db_xref="InterPro:IPR017853"
FT /db_xref="InterPro:IPR023230"
FT /db_xref="InterPro:IPR023232"
FT /db_xref="InterPro:IPR023933"
FT /db_xref="PDB:1BGL"
FT /db_xref="PDB:1BGM"
FT /db_xref="PDB:1DP0"
FT /db_xref="PDB:1F49"
FT /db_xref="PDB:1F4A"
FT /db_xref="PDB:1F4H"
FT /db_xref="PDB:1GHO"
FT /db_xref="PDB:1HN1"
FT /db_xref="PDB:1JYN"
FT /db_xref="PDB:1JYV"
FT /db_xref="PDB:1JYW"
FT /db_xref="PDB:1JYX"
FT /db_xref="PDB:1JYY"
FT /db_xref="PDB:1JYZ"
FT /db_xref="PDB:1JZ0"
FT /db_xref="PDB:1JZ1"
FT /db_xref="PDB:1JZ2"
FT /db_xref="PDB:1JZ3"
FT /db_xref="PDB:1JZ4"
FT /db_xref="PDB:1JZ5"
FT /db_xref="PDB:1JZ6"
FT /db_xref="PDB:1JZ7"
FT /db_xref="PDB:1JZ8"
FT /db_xref="PDB:1PX3"
FT /db_xref="PDB:1PX4"
FT /db_xref="PDB:3CZJ"
FT /db_xref="PDB:3DYM"
FT /db_xref="PDB:3DYO"
FT /db_xref="PDB:3DYP"
FT /db_xref="PDB:3E1F"
FT /db_xref="PDB:3I3B"
FT /db_xref="PDB:3I3D"
FT /db_xref="PDB:3I3E"
FT /db_xref="PDB:3IAP"
FT /db_xref="PDB:3IAQ"
FT /db_xref="PDB:3MUY"
FT /db_xref="PDB:3MUZ"
FT /db_xref="PDB:3MV0"
FT /db_xref="PDB:3MV1"
FT /db_xref="PDB:3SEP"
FT /db_xref="PDB:3T08"
FT /db_xref="PDB:3T09"
FT /db_xref="PDB:3T0A"
FT /db_xref="PDB:3T0B"
FT /db_xref="PDB:3T0D"
FT /db_xref="PDB:3T2O"
FT /db_xref="PDB:3T2P"
FT /db_xref="PDB:3T2Q"
FT /db_xref="PDB:3VD3"
FT /db_xref="PDB:3VD4"
FT /db_xref="PDB:3VD5"
FT /db_xref="PDB:3VD7"
FT /db_xref="PDB:3VD9"
FT /db_xref="PDB:3VDA"
FT /db_xref="PDB:3VDB"
FT /db_xref="PDB:3VDC"
FT /db_xref="UniProtKB/Swiss-Prot:P00722"
FT /protein_id="CAA23570.1"
FT /translation="FQLSAGRYHYQLVWCQK"
FT CDS 106..1359
FT /transl_table=11
FT /note="reading frame (lacY)"
FT /db_xref="GOA:P02920"
FT /db_xref="InterPro:IPR000576"
FT /db_xref="InterPro:IPR016196"
FT /db_xref="InterPro:IPR018457"
FT /db_xref="InterPro:IPR020846"
FT /db_xref="InterPro:IPR022814"
FT /db_xref="PDB:1M2U"
FT /db_xref="PDB:1PV6"
FT /db_xref="PDB:1PV7"
FT /db_xref="PDB:2CFP"
FT /db_xref="PDB:2CFQ"
FT /db_xref="PDB:2V8N"
FT /db_xref="PDB:2Y5Y"
FT /db_xref="UniProtKB/Swiss-Prot:P02920"
FT /protein_id="CAA23571.1"
FT /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDT
FT GIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNIL
FT VGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGIMFT
FT INNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKL
FT WFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPL
FT IINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQ
FT FEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISVFT
FT LSGPGPLSLLRRQVNEVA"
FT CDS 1423..>1500
FT /transl_table=11
FT /note="reading frame (lacA)"
FT /db_xref="GOA:P07464"
FT /db_xref="InterPro:IPR001451"
FT /db_xref="InterPro:IPR011004"
FT /db_xref="InterPro:IPR018357"
FT /db_xref="InterPro:IPR024688"
FT /db_xref="PDB:1KQA"
FT /db_xref="PDB:1KRR"
FT /db_xref="PDB:1KRU"
FT /db_xref="PDB:1KRV"
FT /db_xref="UniProtKB/Swiss-Prot:P07464"
FT /protein_id="CAA23572.1"
FT /translation="MNMPMTERIRAGKLFTDMCEGLPEKR"
XX
SQ Sequence 1500 BP; 315 A; 342 C; 357 G; 486 T; 0 other;
ttccagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa ataataataa 60
ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat ccattatgta ctatttaaaa 120
aacacaaact tttggatgtt cggtttattc tttttctttt acttttttat catgggagcc 180
tacttcccgt ttttcccgat ttggctacat gacatcaacc atatcagcaa aagtgatacg 240
ggtattattt ttgccgctat ttctctgttc tcgctattat tccaaccgct gtttggtctg 300
ctttctgaca aactcgggct gcgcaaatac ctgctgtgga ttattaccgg catgttagtg 360
atgtttgcgc cgttctttat ttttatcttc gggccactgt tacaatacaa cattttagta 420
ggatcgattg ttggtggtat ttatctaggc ttttgtttta acgccggtgc gccagcagta 480
gaggcattta ttgagaaagt cagccgtcgc agtaatttcg aatttggtcg cgcgcggatg 540
tttggctgtg ttggctgggc gctgtgtgcc tcgattgtcg gcatcatgtt caccatcaat 600
aatcagtttg ttttctggct gggctctggc tgtgcactca tcctcgccgt tttactcttt 660
ttcgccaaaa cggatgcgcc ctcttctgcc acggttgcca atgcggtagg tgccaaccat 720
tcggcattta gccttaagct ggcactggaa ctgttcagac agccaaaact gtggtttttg 780
tcactgtatg ttattggcgt ttcctgcacc tacgatgttt ttgaccaaca gtttgctaat 840
ttctttactt cgttctttgc taccggtgaa cagggtacgc gggtatttgg ctacgtaacg 900
acaatgggcg aattacttaa cgcctcgatt atgttctttg cgccactgat cattaatcgc 960
atcggtggga aaaacgccct gctgctggct ggcactatta tgtctgtacg tattattggc 1020
tcatcgttcg ccacctcagc gctggaagtg gttattctga aaacgctgca tatgtttgaa 1080
gtaccgttcc tgctggtggg ctgctttaaa tatattacca gccagtttga agtgcgtttt 1140
tcagcgacga tttatctggt ctgtttctgc ttctttaagc aactggcgat gatttttatg 1200
tctgtactgg cgggcaatat gtatgaaagc atcggtttcc agggcgctta tctggtgctg 1260
ggtctggtgg cgctgggctt caccttaatt tccgtgttca cgcttagcgg ccccggcccg 1320
ctttccctgc tgcgtcgtca ggtgaatgaa gtcgcttaag caatcaatgt cggatgcggc 1380
gcgacgctta tccgaccaac atatcataac ggagtgatcg cattgaacat gccaatgacc 1440
gaaagaataa gagcaggcaa gctatttacc gatatgtgcg aaggcttacc ggaaaaaaga 1500
//
ID V00296; SV 1; linear; genomic DNA; STD; PRO; 3078 BP.
XX
AC V00296;
XX
DT 13-JUL-1983 (Rel. 03, Created)
DT 18-APR-2005 (Rel. 83, Last updated, Version 5)
XX
DE E. coli gene lacZ coding for beta-galactosidase (EC 3.2.1.23).
XX
KW galactosidase.
XX
OS Escherichia coli
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
XX
RN [1]
RP 1-3078
RX PUBMED; 6313347.
RA Kalnins A., Otto K., Ruether U., Mueller-Hill B.;
RT "Sequence of the lacZ gene of Escherichia coli";
RL EMBO J. 2(4):593-597(1983).
XX
RN [2]
RX PUBMED; 3038536.
RA Zell R., Fritz H.J.;
RT "DNA mismatch-repair in Escherichia coli counteracting the hydrolytic
RT deamination of 5-methyl-cytosine residues";
RL EMBO J. 6(6):1809-1815(1987).
XX
CC Data kindly reviewed (18-MAY-1983) by U. Ruether
XX
FH Key Location/Qualifiers
FH
FT source 1..3078
FT /organism="Escherichia coli"
FT /mol_type="genomic DNA"
FT /db_xref="taxon:562"
FT CDS <1..3072
FT /transl_table=11
FT /note="galactosidase"
FT /db_xref="GOA:P00722"
FT /db_xref="InterPro:IPR004199"
FT /db_xref="InterPro:IPR006101"
FT /db_xref="InterPro:IPR006102"
FT /db_xref="InterPro:IPR006103"
FT /db_xref="InterPro:IPR006104"
FT /db_xref="InterPro:IPR008979"
FT /db_xref="InterPro:IPR011013"
FT /db_xref="InterPro:IPR013781"
FT /db_xref="InterPro:IPR013812"
FT /db_xref="InterPro:IPR014718"
FT /db_xref="InterPro:IPR017853"
FT /db_xref="InterPro:IPR023230"
FT /db_xref="InterPro:IPR023232"
FT /db_xref="InterPro:IPR023933"
FT /db_xref="PDB:1BGL"
FT /db_xref="PDB:1BGM"
FT /db_xref="PDB:1DP0"
FT /db_xref="PDB:1F49"
FT /db_xref="PDB:1F4A"
FT /db_xref="PDB:1F4H"
FT /db_xref="PDB:1GHO"
FT /db_xref="PDB:1HN1"
FT /db_xref="PDB:1JYN"
FT /db_xref="PDB:1JYV"
FT /db_xref="PDB:1JYW"
FT /db_xref="PDB:1JYX"
FT /db_xref="PDB:1JYY"
FT /db_xref="PDB:1JYZ"
FT /db_xref="PDB:1JZ0"
FT /db_xref="PDB:1JZ1"
FT /db_xref="PDB:1JZ2"
FT /db_xref="PDB:1JZ3"
FT /db_xref="PDB:1JZ4"
FT /db_xref="PDB:1JZ5"
FT /db_xref="PDB:1JZ6"
FT /db_xref="PDB:1JZ7"
FT /db_xref="PDB:1JZ8"
FT /db_xref="PDB:1PX3"
FT /db_xref="PDB:1PX4"
FT /db_xref="PDB:3CZJ"
FT /db_xref="PDB:3DYM"
FT /db_xref="PDB:3DYO"
FT /db_xref="PDB:3DYP"
FT /db_xref="PDB:3E1F"
FT /db_xref="PDB:3I3B"
FT /db_xref="PDB:3I3D"
FT /db_xref="PDB:3I3E"
FT /db_xref="PDB:3IAP"
FT /db_xref="PDB:3IAQ"
FT /db_xref="PDB:3MUY"
FT /db_xref="PDB:3MUZ"
FT /db_xref="PDB:3MV0"
FT /db_xref="PDB:3MV1"
FT /db_xref="PDB:3SEP"
FT /db_xref="PDB:3T08"
FT /db_xref="PDB:3T09"
FT /db_xref="PDB:3T0A"
FT /db_xref="PDB:3T0B"
FT /db_xref="PDB:3T0D"
FT /db_xref="PDB:3T2O"
FT /db_xref="PDB:3T2P"
FT /db_xref="PDB:3T2Q"
FT /db_xref="PDB:3VD3"
FT /db_xref="PDB:3VD4"
FT /db_xref="PDB:3VD5"
FT /db_xref="PDB:3VD7"
FT /db_xref="PDB:3VD9"
FT /db_xref="PDB:3VDA"
FT /db_xref="PDB:3VDB"
FT /db_xref="PDB:3VDC"
FT /db_xref="UniProtKB/Swiss-Prot:P00722"
FT /protein_id="CAA23573.1"
FT /translation="TMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTD
FT RPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVT
FT YPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQ
FT DSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQI
FT SDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDE
FT RGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENG
FT LLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWY
FT TLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSL
FT GNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPK
FT WSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLI
FT KYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQT
FT IEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQL
FT WLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGN
FT KRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKA
FT AGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVE
FT VASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYV
FT FPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGF
FT HMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
FT mat_peptide 1..3069
XX
SQ Sequence 3078 BP; 682 A; 841 C; 886 G; 669 T; 0 other;
accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 60
gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 120
gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt 180
gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag 240
gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac 300
accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg 360
ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga 420
attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt 480
tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga 540
gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct ggaagatcag 600
gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca 660
caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg 720
gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta 780
tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat 840
gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg 900
tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc 960
acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt 1020
ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat 1080
cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag 1140
cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg 1200
ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg 1260
gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta 1320
acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat 1380
gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct 1440
tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc 1500
ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc 1560
aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac 1620
gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc 1680
cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa 1740
aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag 1800
ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca 1860
aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc 1920
gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt 1980
aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt 2040
gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta 2100
gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg 2160
cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg 2220
accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc 2280
cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg 2340
cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc 2400
attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca 2460
gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac 2520
gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt 2580
agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg 2640
cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta 2700
gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg 2760
ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg 2820
acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc 2880
cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa 2940
ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc 3000
ccgtcagtat cggcggaatt ccagctgagc gccggtcgct accattacca gttggtctgg 3060
tgtcaaaaat aataataa 3078
//
ID V00307; SV 1; linear; genomic DNA; STD; PRO; 2271 BP.
XX
AC V00307; J01654;
XX
DT 09-JUN-1982 (Rel. 01, Created)
DT 01-OCT-1996 (Rel. 49, Last updated, Version 8)
XX
DE E. coli ompA gene (codes for the outer membrane protein II*).
XX
KW membrane protein; unidentified reading frame.
XX
OS Escherichia coli
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC Enterobacteriaceae; Escherichia.
XX
RN [1]
RP 1-2271
RX DOI; 10.1093/nar/8.13.3011.
RX PUBMED; 6253901.
RA Beck E., Bremer E.;
RT "Nucleotide sequence of the gene ompA coding the outer membrane protein II
RT of Escherichia coli K-12";
RL Nucleic Acids Res. 8(13):3011-3027(1980).
XX
RN [2]
RP 783-1317
RX DOI; 10.1073/pnas.77.7.3845.
RX PUBMED; 7001445.
RA Movva N.R., Nakamura K., Inouye M.;
RT "Regulatory region of the gene for the ompA protein, a major outer membrane
RT protein of Escherichia coli";
RL Proc. Natl. Acad. Sci. U.S.A. 77(7):3845-3849(1980).
XX
DR GOA; P0A8N0.
DR InterPro; IPR009390; Ter_macrodom_MatS-bd_MatP.
DR UniProtKB/Swiss-Prot; P0A8N0; MATP_ECOLI.
XX
CC KST ECO.OMPA
XX
FH Key Location/Qualifiers
FH
FT source 1..2271
FT /organism="Escherichia coli"
FT /mol_type="genomic DNA"
FT /db_xref="taxon:562"
FT CDS 172..669
FT /transl_table=11
FT /note="reading frame (unknown protein)"
FT /db_xref="GOA:P0AFZ5"
FT /db_xref="InterPro:IPR004596"
FT /db_xref="UniProtKB/Swiss-Prot:P0AFZ5"
FT /protein_id="CAA23587.1"
FT /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPMM
FT TQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVESMV
FT RALRTGNYSVVIGWLADDLTEEEHAEVVDAANEGNAMGFIIHSGKRILSRHETTFRAKN
FT SL"
FT misc_difference 906..906
FT /note="may be missing [2]"
FT /note="conflict"
FT /citation=[2]
FT CDS 1037..2077
FT /transl_table=11
FT /gene="ompA"
FT /product="ompA protein"
FT /db_xref="GOA:P0A910"
FT /db_xref="InterPro:IPR000498"
FT /db_xref="InterPro:IPR002368"
FT /db_xref="InterPro:IPR006664"
FT /db_xref="InterPro:IPR006665"
FT /db_xref="InterPro:IPR006690"
FT /db_xref="InterPro:IPR011250"
FT /db_xref="PDB:1BXW"
FT /db_xref="PDB:1G90"
FT /db_xref="PDB:1QJP"
FT /db_xref="PDB:2GE4"
FT /db_xref="PDB:2JMM"
FT /db_xref="PDB:3NB3"
FT /db_xref="UniProtKB/Swiss-Prot:P0A910"
FT /protein_id="CAA23588.1"
FT /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGFI
FT NNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTAKL
FT GYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRLEYQ
FT WTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLKSDVL
FT FNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSERRAQSV
FT VDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEVKGIKDV
FT VTQPQA"
XX
SQ Sequence 2271 BP; 567 A; 552 C; 548 G; 604 T; 0 other;
gatccgttaa ctacgaaaat aggcaactta ttcttaaggg gcaagattaa tttatgtttt 60
cccgtcacca acgacaaaat ttgcgaggct ctttccgaaa atagggttga tctttgttgt 120
cactggatgt actgtacatc catacagtaa ctcacagggg ctggattgat tatgtacact 180
tcaggctatg cacatcgttc ttcgtcgttc tcatccgcag caagtaaaat tgcgcgtgtc 240
tctacggaaa acactacagc cgggcttatc agtgaagttg tctatcgcga agatcagccc 300
atgatgacgc aacttctact gttgccattg ttacagcaac tcggtcagca atcgcgctgg 360
caactctggt taacaccgca acaaaaactg agtcgggaat gggttcaggc atctgggcta 420
cccttaacga aagtaatgca gattagccag ctctcccctt gccacactgt ggagtcaatg 480
gttcgcgctt tacgcacggg caattacagt gtggtgatcg gttggttggc agatgatttg 540
actgaagaag agcatgctga agttgttgat gcggcaaatg aaggtaacgc tatggggttt 600
attatacatt ccggtaagcg catcctctca cgccacgaga caactttccg ggctaaaaat 660
tcactctaat ttgtatcatt aagtaaattt aggattaatc ctggaacttt ttttgtcgcc 720
cagccaatgc tttcagtcgt gactaatttc cttgcggagg cttgtctgag cggtttccgc 780
gattctcttc tgtaaattgt cgctgacaaa aaagattaaa cataccttat acaagacttt 840
tttttcatat gcctgacgga gttcacactt gtaagttttc aactacgttg tagactttac 900
atcgccaagg gtgctcggca taagccgaag atatcggtag agttaatatt gagcagatcc 960
cccggtgaag gatttaaccg tgttatctcg ttggagatat tcatggcgta ttttggatga 1020
taacgaggcg caaaaaatga aaaagacagc tatcgcgatt gcagtggcac tggctggttt 1080
cgctaccgta gcgcaggccg ctccgaaaga taacacctgg tacactggtg ctaaactggg 1140
ctggtcccag taccatgata ctggtttcat caacaacaat ggcccgaccc atgaaaacca 1200
actgggcgct ggtgcttttg gtggttacca ggttaacccg tatgttggct ttgaaatggg 1260
ttacgactgg ttaggtcgta tgccgtacaa aggcagcgtt gaaaacggtg catacaaagc 1320
tcagggcgtt caactgaccg ctaaactggg ttacccaatc actgacgacc tggacatcta 1380
cactcgtctg ggtggcatgg tatggcgtgc agacactaaa tccaacgttt atggtaaaaa 1440
ccacgacacc ggcgtttctc cggtcttcgc tggcggtgtt gagtacgcga tcactcctga 1500
aatcgctacc cgtctggaat accagtggac gaacaacatc ggtgacgcac acaccatcgg 1560
cactcgtccg gacaacggca tgctgagcct gggtgtttcc taccgtttcg gtcagggcga 1620
ggcagctcca gtagttgctc cggctccagc tccggcaccg gaagtacaga ccaagcactt 1680
cactctgaag tctgacgttc tgttcaactt caacaaagca accctgaaac cggaaggtca 1740
ggctgctctg gatcagctgt acagccagct gagcaacttg gatccgaaag acggttccgt 1800
agttgttctg ggttacaccg accgcatcgg ttctgacgct tacaaccagg gtctgtccga 1860
gcgccgtgct cagtctgttg ttgattacct gatctccaaa ggtatcccgg cagacaagat 1920
ctccgcacgt ggtatgggcg aatccaaccc ggttactggc aacacctgtg acaacgtgaa 1980
acagcgtgct gcactgatcg actgcctggc tccggatcgt cgcgtagaga tcgaagttaa 2040
aggtatcaaa gacgttgtaa ctcagccgca ggcttaagtt ctcgtctggt agaaaaacgc 2100
tgctgcgggt ttttttttgc ctttagtaaa ttgaactgac tttcgtcagt tattccttac 2160
ccagcaatgc ctgcagatcc tgcttcagag aagacatttt attcgcgtat ttctctttgt 2220
tttccgcatc ttcaatcagc tgaacaatcg tttcagaaag cgttttaccg c 2271
//
ID X77160; SV 1; linear; genomic DNA; STD; PRO; 1212 BP.
XX
AC X77160;
XX
DT 13-APR-1994 (Rel. 39, Created)
DT 18-APR-2005 (Rel. 83, Last updated, Version 8)
XX
DE Pseudomonas aeruginosa (PAC1) amiB gene.
XX
KW amiB gene.
XX
OS Pseudomonas aeruginosa
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
XX
RN [1]
RA Wilson S.A., Wachira S.M., Drew R.E., Jones D., Pearl L.H.;
RT "Identification of a putative ABC transporter in the Pseudomonas aeruginosa
RT amidase operon";
RL Unpublished.
XX
RN [2]
RP 1-1212
RA Wilson S.A.;
RT ;
RL Submitted (17-JAN-1994) to the INSDC.
RL S.A. Wilson, University College London, Dept of Biochemistry, Gower Street,
RL London WC1E 6BT, UK
XX
RN [3]
RP 1-1212
RX DOI; 10.1074/jbc.270.32.18818.
RX PUBMED; 7642533.
RA Wilson S.A., Williams R.J., Pearl L.H., Drew R.E.;
RT "Identification of two new genes in the Pseudomonas aeruginosa amidase
RT operon, encoding an ATPase (AmiB) and a putative integral membrane protein
RT (AmiS)";
RL J Biol Chem 270(32):18818-18824(1995).
XX
DR StrainInfo; 345027; 0.
XX
FH Key Location/Qualifiers
FH
FT source 1..1212
FT /organism="Pseudomonas aeruginosa"
FT /strain="PAC1"
FT /mol_type="genomic DNA"
FT /clone="plasmid pAS20"
FT /db_xref="taxon:287"
FT CDS 97..1212
FT /transl_table=11
FT /gene="amiB"
FT /db_xref="GOA:Q51416"
FT /db_xref="InterPro:IPR001270"
FT /db_xref="InterPro:IPR003593"
FT /db_xref="InterPro:IPR013093"
FT /db_xref="InterPro:IPR019489"
FT /db_xref="UniProtKB/Swiss-Prot:Q51416"
FT /protein_id="CAA54405.1"
FT /translation="MPFLSDMLDQSRRQQDEEQALARENLAEASLLQAHLSHRSALHSR
FT FRFDPAAVMDCLRAEVLGQEPALQAVEDMLKVVRADIADPRRPLFSALFLGPTGVGKTE
FT IVRALARALHGDAEGFCRVDMNTLSQEHYAAALTGAPPGYVGAKEGTTLLEQDKLDGSP
FT GRPGIVLFDELEKASPEVVHALLNVLDNGLLRVASGERTYHFRNTLVFMTSNLCAHEIQ
FT RYDERRQRLPWRLLPVGGERRRRDIDGMVRARLLKTFSPEFVNRLDSVVTFNWIERDVV
FT ARLVELEVQRLNRRLEKHRCRLEATPEVLAKIARAGFDRQFGARALRRSVRHHLEVPLA
FT EHLLDHHQPGDGNCTIYLASLEHERVRFVRR"
XX
SQ Sequence 1212 BP; 193 A; 426 C; 403 G; 190 T; 0 other;
gaaggaagcc tgacggcaga cgccgccagc ccggcgtgcc gtcgtgcggc acgccgtctc 60
cgccatttcc cccctgtggc agaaggagtt tcatccatgc cttttctgag cgacatgctc 120
gaccagtccc gccggcagca ggacgaggaa caggccctgg cgcgggagaa tcttgccgag 180
gcaagtctgc tccaggccca cctgagtcac cgcagcgccc tgcacagccg tttccgtttc 240
gacccggcgg cggtgatgga ctgcctgcgc gccgaggtgc tcggccagga accggcgcta 300
caggccgtcg aggacatgct caaggtggtt cgcgcggaca tcgccgaccc gcgccgtccg 360
ctgttcagcg cgctgttcct cggccccacc ggagtcggca agaccgagat cgtgcgcgcc 420
ctggccaggg ccctgcacgg cgacgccgag gggttctgcc gggtggacat gaacaccctg 480
tcccaggagc actatgccgc cgccctcacc ggtgcgccgc cgggctacgt cggggcgaag 540
gagggcacca ccctgttgga gcaggacaag ctggacggca gtcccgggcg ccccggcatc 600
gttctcttcg acgaactgga aaaggccagc ccggaagtgg tccatgcgtt gctcaacgta 660
ctcgacaacg gcctgctacg ggtcgcttcc ggcgaacgca cctaccattt ccgcaacacc 720
ctggtgttca tgaccagcaa tctctgcgcc catgagatcc agcgctacga cgagcgtcgc 780
cagcgcctgc cctggcgcct gctgccggtc ggcggcgagc gccggcggcg ggacatcgac 840
gggatggtcc gggcccggct gctgaagacc ttctcgccgg agttcgtcaa tcgtctcgat 900
agcgtggtca ccttcaactg gatcgaacgc gacgtcgtcg cgcgcctggt cgagctggag 960
gtgcagcggc tcaaccggcg cctggagaag catcgctgcc gcctggaggc gaccccggag 1020
gtgctggcga agatcgcccg cgccggcttc gaccggcagt tcggcgcccg tgcgttgcgc 1080
cgcagcgtcc gtcatcatct cgaggtaccg ctggccgagc atctgctcga tcaccaccag 1140
ccgggcgacg ggaactgcac gatctacctg gcgagcctgg agcacgagcg ggttcgcttc 1200
gtacggcgct ga 1212
//
ID M27612; SV 1; linear; genomic DNA; STD; PRO; 1065 BP.
XX
AC M27612;
XX
DT 02-FEB-1990 (Rel. 22, Created)
DT 22-APR-2002 (Rel. 71, Last updated, Version 4)
XX
DE Pseudomonas aeruginosa aliphatic amidase (amiE) gene, complete cds.
XX
KW .
XX
OS Pseudomonas aeruginosa
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
XX
RN [1]
RP 1-1065
RX DOI; 10.1016/0014-5793(87)80164-3.
RX PUBMED; 3108030.
RA Brammar W.J., Charles I.G., Matfield M., Liu C.P., Drew R.E., Clarke P.H.;
RT "The nucleotide sequence of the amiE gene of Pseudomonas aeruginosa";
RL FEBS Lett. 215(2):291-294(1987).
XX
FH Key Location/Qualifiers
FH
FT source 1..1065
FT /organism="Pseudomonas aeruginosa"
FT /strain="PAC433"
FT /mol_type="genomic DNA"
FT /db_xref="taxon:287"
FT CDS 25..1065
FT /codon_start=1
FT /transl_table=11
FT /gene="amiE"
FT /product="aliphatic amidase"
FT /EC_number="3.5.1.4"
FT /db_xref="GOA:P11436"
FT /db_xref="InterPro:IPR003010"
FT /db_xref="InterPro:IPR023719"
FT /db_xref="PDB:1K17"
FT /db_xref="PDB:2UXY"
FT /db_xref="UniProtKB/Swiss-Prot:P11436"
FT /protein_id="AAA25697.1"
FT /translation="MRHGDISSSNDTVGVAVVNYKMPRLHTAAEVLDNARKIADMIVGM
FT KQGLPGMDLVVFPEYSLQGIMYDPAEMMETAVAIPGEETEIFSRACRKANVWGVFSLTG
FT ERHEEHPRKAPYNTLVLIDNNGEIVQKYRKIIPWCPIEGWYPGGQTYVSEGPKGMKISL
FT IICDDPNYPEIWRDCAMKGAELIVRCQGYMYPAKDQQVMMAKAMAWANNCYVAVANAAG
FT FDGVYSYFGHSAIIGFDGRTLGECGEEEMGIQYAQLSLSQIRDARANDQSQNHLFKILH
FT RGYSGLQASGDGDRGLAECPFEFYRTWVTDAEKARDNVERLTRSTTGVAQCPVGRLPYE
FT GLEKEA"
XX
SQ Sequence 1065 BP; 220 A; 337 C; 343 G; 165 T; 0 other;
aaataacaac aggaggtgat acccatgcgt cacggcgata tttccagcag caacgacacc 60
gtcggagtgg ccgtggtcaa ctacaagatg ccgcgcctgc acaccgcggc ggaggtcctg 120
gacaacgccc ggaagatcgc cgacatgatc gtcggcatga agcagggcct gcccggcatg 180
gacctggtgg tgttcccgga gtacagcctg cagggcatca tgtacgatcc ggcggagatg 240
atggaaaccg cggtggcgat ccccggcgag gaaaccgaga tattctcccg cgcctgccgc 300
aaggccaacg tctggggcgt attctccctc accggcgaac ggcacgagga gcatccgcgc 360
aaggcgccgt acaacaccct ggtgctgatc gacaacaacg gcgagatcgt ccagaagtac 420
cgcaagatca ttccctggtg ccccatcgag ggctggtatc ccggtggcca gacctacgtc 480
agcgaagggc cgaagggcat gaagatcagc ctgatcatct gcgacgaccc caattacccg 540
gagatctggc gcgactgcgc gatgaagggc gccgagctga tcgtgcgctg ccagggctac 600
atgtacccgg ccaaggacca gcaggtgatg atggccaagg ccatggcctg ggccaacaac 660
tgctatgtgg cggtggccaa cgcggccggc ttcgacggtg tctattccta cttcggccac 720
tcggcgatca tcggcttcga cggccgtacc ctcggtgagt gcggcgagga ggaaatgggt 780
atccagtacg cccagctgtc cctttcgcag atccgcgatg cgcgcgccaa cgatcagtcg 840
cagaaccacc tgttcaagat cctccaccgc ggctacagcg gcttgcaggc gtccggcgac 900
ggcgaccggg gcctggcgga gtgtccgttc gagttctacc gcacctgggt caccgacgcc 960
gagaaggcgc gcgacaatgt cgagcgactg acccgctcga ccaccggcgt ggcgcaatgc 1020
ccggtcggcc ggctgcccta tgagggactg gagaaggaag cctga 1065
//
ID X13776; SV 1; linear; genomic DNA; STD; PRO; 2167 BP.
XX
AC X13776; M43175;
XX
DT 19-APR-1989 (Rel. 19, Created)
DT 14-NOV-2006 (Rel. 89, Last updated, Version 24)
XX
DE Pseudomonas aeruginosa amiC and amiR gene for aliphatic amidase regulation
XX
KW aliphatic amidase regulator; amiC gene; amiR gene.
XX
OS Pseudomonas aeruginosa
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
XX
RN [1]
RP 1167-2167
RA Rice P.M.;
RT ;
RL Submitted (16-DEC-1988) to the INSDC.
RL Rice P.M., EMBL, Postfach 10-2209, Meyerhofstrasse 1, 6900 Heidelberg, FRG.
XX
RN [2]
RP 1167-2167
RX DOI; 10.1016/0014-5793(89)80249-2.
RX PUBMED; 2495988.
RA Lowe N., Rice P.M., Drew R.E.;
RT "Nucleotide sequence of the aliphatic amidase regulator gene (amiR) of
RT Pseudomonas aeruginosa";
RL FEBS Lett. 246(1-2):39-43(1989).
XX
RN [3]
RP 1-1292
RX PUBMED; 1907262.
RA Wilson S., Drew R.;
RT "Cloning and DNA sequence of amiC, a new gene regulating expression of the
RT Pseudomonas aeruginosa aliphatic amidase, and purification of the amiC
RT product";
RL J. Bacteriol. 173(16):4914-4921(1991).
XX
RN [4]
RP 1-2167
RA Rice P.M.;
RT ;
RL Submitted (04-SEP-1991) to the INSDC.
RL Rice P.M., EMBL, Postfach 10-2209, Meyerhofstrasse 1, 6900 Heidelberg, FRG.
XX
DR GOA; Q51417.
DR InterPro; IPR003211; AmiSUreI_transpt.
DR UniProtKB/Swiss-Prot; Q51417; AMIS_PSEAE.
XX
CC Data kindly reviewed (04-SEP-1991) by Rice P. M.
XX
FH Key Location/Qualifiers
FH
FT source 1..2167
FT /organism="Pseudomonas aeruginosa"
FT /map="38 min"
FT /strain="PAC"
FT /isolate="PAC 1"
FT /mol_type="genomic DNA"
FT /db_xref="taxon:287"
FT CDS 1289..1879
FT /transl_table=11
FT /gene="amiR"
FT /note="aliphatic amidase regulator, positive regulator of
FT amiE"
FT /db_xref="GOA:P10932"
FT /db_xref="InterPro:IPR005561"
FT /db_xref="InterPro:IPR008327"
FT /db_xref="InterPro:IPR011006"
FT /db_xref="InterPro:IPR011991"
FT /db_xref="PDB:1QO0"
FT /db_xref="UniProtKB/Swiss-Prot:P10932"
FT /protein_id="CAA32023.1"
FT /translation="MSANSLLGSLRELQVLVLNPPGEVSDALVLQLIRIGCSVRQCWPP
FT PEAFDVPVDVVFTSIFQNGHHDEIAALLAAGTPRTTLVALVEYESPAVLSQIIELECHG
FT VITQPLDAHRVLPVLVSARRISEEMAKLKQKTEQLQDRIAGQARINQAKVLLMQRHGWD
FT EREAHQHLSREAMKRREPILKIAQELLGNEPSA"
FT CDS 135..1292
FT /transl_table=11
FT /gene="amiC"
FT /note="negative regulator of amiR"
FT /db_xref="GOA:P27017"
FT /db_xref="InterPro:IPR000709"
FT /db_xref="PDB:1PEA"
FT /db_xref="PDB:1QNL"
FT /db_xref="PDB:1QO0"
FT /db_xref="UniProtKB/Swiss-Prot:P27017"
FT /protein_id="CAA32024.1"
FT /translation="MGSHQERPLIGLLFSETGVTADIERSHAYGALLAVEQLNREGGVG
FT GRPIETLSQDPGGDPDRYRLCAEDFIRNRGVRFLVGCYMSHTRKAVMPVVERADALLCY
FT PTPYEGFEYSPNIVYGGPAPNQNSAPLAAYLIRHYGERVVFIGSDYIYPRESNHVMRHL
FT YRQHGGTVLEEIYIPLYPSDDDLQRAVERIYQARADVVFSTVVGTGTAELYRAIARRYG
FT DGRRPPIASLTTSEAEVAKMESDVAEGQVVVAPYFSSIDTPASRAFVQACHGFFPENAT
FT ITAWAEAAYWQTLLLGRAAQAAGNWRVEDVQRHLYDIDIDAPQGPVRVERQNNHSRLSS
FT RIAEIDARGVFQVRWQSPEPIRPDPYVVVHNLDDWSASMGGGPLP"
FT promoter 8..24
FT /note="proposed rpoN-dependent promoter"
FT promoter 65..81
FT /note="proposed rpoN-dependent promoter"
FT RBS 121..126
FT /note="proposed Shine-Dalgarno sequence"
FT variation 912..1167
FT /gene="amiC"
FT /replace=""
FT /note="ClaI fragment deleted in pSW36, constitutive
FT phenotype"
FT misc_feature 1
FT /note="last base of an XhoI site"
FT misc_feature 648..653
FT /note="end of 658bp XhoI fragment, deletion in pSW3 causes
FT constitutive expression of amiE"
FT misc_difference 1281
FT /replace="g"
FT /note="conflict"
FT /citation=[3]
XX
SQ Sequence 2167 BP; 363 A; 712 C; 730 G; 362 T; 0 other;
ggtaccgctg gccgagcatc tgctcgatca ccaccagccg ggcgacggga actgcacgat 60
ctacctggcg agcctggagc acgagcgggt tcgcttcgta cggcgctgag cgacagtcac 120
aggagaggaa acggatggga tcgcaccagg agcggccgct gatcggcctg ctgttctccg 180
aaaccggcgt caccgccgat atcgagcgct cgcacgcgta tggcgcattg ctcgcggtcg 240
agcaactgaa ccgcgagggc ggcgtcggcg gtcgcccgat cgaaacgctg tcccaggacc 300
ccggcggcga cccggaccgc tatcggctgt gcgccgagga cttcattcgc aaccgggggg 360
tacggttcct cgtgggctgc tacatgtcgc acacgcgcaa ggcggtgatg ccggtggtcg 420
agcgcgccga cgcgctgctc tgctacccga ccccctacga gggcttcgag tattcgccga 480
acatcgtcta cggcggtccg gcgccgaacc agaacagtgc gccgctggcg gcgtacctga 540
ttcgccacta cggcgagcgg gtggtgttca tcggctcgga ctacatctat ccgcgggaaa 600
gcaaccatgt gatgcgccac ctgtatcgcc agcacggcgg cacggtgctc gaggaaatct 660
acattccgct gtatccctcc gacgacgact tgcagcgcgc cgtcgagcgc atctaccagg 720
cgcgcgccga cgtggtcttc tccaccgtgg tgggcaccgg caccgccgag ctgtatcgcg 780
ccatcgcccg tcgctacggc gacggcaggc ggccgccgat cgccagcctg accaccagcg 840
aggcggaggt ggcgaagatg gagagtgacg tggcagaggg gcaggtggtg gtcgcgcctt 900
acttctccag catcgatacg cccgccagcc gggccttcgt ccaggcctgc catggtttct 960
tcccggagaa cgcgaccatc accgcctggg ccgaggcggc ctactggcag accttgttgc 1020
tcggccgcgc cgcgcaggcc gcaggcaact ggcgggtgga agacgtgcag cggcacctgt 1080
acgacatcga catcgacgcg ccacaggggc cggtccgggt ggagcgccag aacaaccaca 1140
gccgcctgtc ttcgcgcatc gcggaaatcg atgcgcgcgg cgtgttccag gtccgctggc 1200
agtcgcccga accgattcgc cccgaccctt atgtcgtcgt gcataacctc gacgactggt 1260
ccgccagcat gggcggggga ccgctcccat gagcgccaac tcgctgctcg gcagcctgcg 1320
cgagttgcag gtgctggtcc tcaacccgcc gggggaggtc agcgacgccc tggtcttgca 1380
gctgatccgc atcggttgtt cggtgcgcca gtgctggccg ccgccggaag ccttcgacgt 1440
gccggtggac gtggtcttca ccagcatttt ccagaatggc caccacgacg agatcgctgc 1500
gctgctcgcc gccgggactc cgcgcactac cctggtggcg ctggtggagt acgaaagccc 1560
cgcggtgctc tcgcagatca tcgagctgga gtgccacggc gtgatcaccc agccgctcga 1620
tgcccaccgg gtgctgcctg tgctggtatc ggcgcggcgc atcagcgagg aaatggcgaa 1680
gctgaagcag aagaccgagc agctccagga ccgcatcgcc ggccaggccc ggatcaacca 1740
ggccaaggtg ttgctgatgc agcgccatgg ctgggacgag cgcgaggcgc accagcacct 1800
gtcgcgggaa gcgatgaagc ggcgcgagcc gatcctgaag atcgctcagg agttgctggg 1860
aaacgagccg tccgcctgag cgatccgggc cgaccagaac aataacaaga ggggtatcgt 1920
catcatgctg ggactggttc tgctgtacgt tggcgcggtg ctgtttctca atgccgtctg 1980
gttgctgggc aagatcagcg gtcgggaggt ggcggtgatc aacttcctgg tcggcgtgct 2040
gagcgcctgc gtcgcgttct acctgatctt ttccgcagca gccgggcagg gctcgctgaa 2100
ggccggagcg ctgaccctgc tattcgcttt tacctatctg tgggtggccg ccaaccagtt 2160
cctcgag 2167
//
ID X77161; SV 1; linear; genomic DNA; STD; PRO; 1130 BP.
XX
AC X77161;
XX
DT 13-APR-1994 (Rel. 39, Created)
DT 31-JUL-2003 (Rel. 76, Last updated, Version 7)
XX
DE Pseudomonas aeruginosa (PAC1) amiS gene.
XX
KW amiS gene.
XX
OS Pseudomonas aeruginosa
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
XX
RN [1]
RA Wilson S.A., Wachira S.M., Drew R.E., Jones D., Pearl L.H.;
RT "Identification of a putative ABC transporter in the Pseudomonas aeruginosa
RT amidase operon";
RL Unpublished.
XX
RN [2]
RP 1-1130
RA Wilson S.A.;
RT ;
RL Submitted (17-JAN-1994) to the INSDC.
RL S.A. Wilson, University College London, Dept of Biochemistry, Gower Street,
RL London WC1E 6BT, UK
XX
RN [3]
RP 1-1130
RX DOI; 10.1074/jbc.270.32.18818.
RX PUBMED; 7642533.
RA Wilson S.A., Williams R.J., Pearl L.H., Drew R.E.;
RT "Identification of two new genes in the Pseudomonas aeruginosa amidase
RT operon, encoding an ATPase (AmiB) and a putative integral membrane protein
RT (AmiS)";
RL J Biol Chem 270(32):18818-18824(1995).
XX
DR StrainInfo; 345027; 0.
XX
FH Key Location/Qualifiers
FH
FT source 1..1130
FT /organism="Pseudomonas aeruginosa"
FT /strain="PAC1"
FT /mol_type="genomic DNA"
FT /clone="plasmid pAS20"
FT /db_xref="taxon:287"
FT CDS 61..576
FT /transl_table=11
FT /gene="amiS"
FT /db_xref="GOA:Q51417"
FT /db_xref="InterPro:IPR003211"
FT /db_xref="UniProtKB/Swiss-Prot:Q51417"
FT /protein_id="CAA54406.1"
FT /translation="MLGLVLLYVGAVLFLNAVWLLGKISGREVAVINFLVGVLSACVAF
FT YLIFSAAAGQGSLKAGALTLLFAFTYLWVAANQFLEVDGKGLGWFCLFVSLTACTVAIE
FT SFAGASGPFGLWNAVNWTVWALLWFCFFLLLGLSRGIQKPVAYLTLASAIFTAWLPGLL
FT LLGQVLKA"
XX
SQ Sequence 1130 BP; 167 A; 335 C; 377 G; 251 T; 0 other;
gagccgtccg cctgagcgat ccgggccgac cagaacaata acaagagggg tatcgtcatc 60
atgctgggac tggttctgct gtacgttggc gcggtgctgt ttctcaatgc cgtctggttg 120
ctgggcaaga tcagcggtcg ggaggtggcg gtgatcaact tcctggtcgg cgtgctgagc 180
gcctgcgtcg cgttctacct gatcttttcc gcagcagccg ggcagggctc gctgaaggcc 240
ggagcgctga ccctgctatt cgcttttacc tatctgtggg tggccgccaa ccagttcctc 300
gaggtggacg gcaagggcct cggctggttc tgcctgttcg tcagcctcac cgcctgcacc 360
gtggcgatcg agtcgttcgc cggcgccagt ggtccgttcg gcctgtggaa cgcggtcaac 420
tggacagtct gggcgttgct ctggttctgt ttcttcctgc tgctggggct gtcccgcggc 480
atccagaagc cggtggccta cctgaccctg gccagcgcca tattcaccgc ctggttgccc 540
ggcctgctgc tgctcggaca ggtgctcaag gcatagcagg aagtcggaaa gggatgacgg 600
cttgccgcca tcccgtccct tccgaacgcc tagccgagcg gccagttgat caccacgacg 660
gcgtcgttgt agtcgttgtc ggtgccgtct tcagagccga ccagggcgaa gttcagctcg 720
ttggtcagga ttacctgtgc cgagaccaga tccgaggggc ggccgttgac gctgacctgg 780
acctgtacct tgccactgct gccggagttg agcacctggg tgccgatgac ggcgttattg 840
gtgctttgcc cgctgaaggt cgcggccgtg ctcgttgttg accagcacgt tcaccgtctg 900
ggttccggac gagttggcga aggcggtgac gccggaacct ggttgttggc gggaagggtg 960
aacactcctt gtggttgcca tggtggtatc tccactgaat acctggcccc ttccttttca 1020
ggcagccgtc tggcgcgcgg tatggcgtgt cgggagaaat ccgcagtcct tggcggcagg 1080
cgatgcgcag gcaggaagga cgcatcgttc agccaatcta cgccgtcgac 1130
//
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