/usr/share/arden/arden-analyze is in arden 1.0-1.
This file is owned by root:root, with mode 0o755.
The actual contents of the file can be viewed below.
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"""
Created on Thu Aug 18 2012
Script for analyzing mapping results using an artificial reference genome (ART / ARG). An exhaustive comparison is performed between the input files (SAM format).
The goal is to calculate a ROC curve and the AUC value to compare different read mapper / different settings on the same read mapper. Therefore FP and TP have to be identified.
The TP are from the original reference. The FP are from the ART and are defined as unique mappings in a sense that no FP alignment occurs on the reference.
@author: Sven Giese
"""
"""
Copyright (c) 2012, Sven H. Giese, gieseS@rki.de,
Robert Koch-Institut, Germany,
All rights reserved.
Redistribution and use in source and binary forms, with or without
modification, are permitted provided that the following conditions are met:
* Redistributions of source code must retain the above copyright
notice, this list of conditions and the following disclaimer.
* Redistributions in binary form must reproduce the above copyright
notice, this list of conditions and the following disclaimer in the
documentation and/or other materials provided with the distribution.
* The name of the author may not be used to endorse or promote products
derived from this software without specific prior written permission.
THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND
ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED
WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE
DISCLAIMED. IN NO EVENT SHALL MARTIN S. LINDNER BE LIABLE FOR ANY
DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES
(INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES;
LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND
ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT
(INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS
SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.
"""
import time
import cProfile
import sys
from optparse import OptionParser
import os
from core import AnalyseMapping as AM
from core import PlotData as PD
def TestFiles(input):
"""Function which checks if all required files are correctly specified (existing) """
controlDic = AM.readInput(input)
# indicator variable
error = 0
for mapper in controlDic["mapper"].keys():
# does the file exist?
try:
# yes
fobj = open(controlDic["mapper"][mapper][0][0],"r")
fobj.close()
except IOError:
# nope --> error
print "error opening file %s. Does it exist?" % controlDic["mapper"][mapper][0][0]
error =1
# do the same for the artificial reference mappings
for p,artificial in enumerate(controlDic["mapper"][mapper][1]):
try:
fobj = open(artificial,"r")
fobj.close()
except IOError:
print "error opening file %s. Does it exist?" % artificial
error =1
return(error)
def main():
""" Control function for arden-analyze """
usage = """usage: %prog [options] [INPUT FILE] [OUTPUTFOLDER] ...
Required Arguments:
OUTPUTFOLDER:\t path to output destination folder.
INPUT FILE: \t ppath to ini file. To get the required format have a look at the example files.
Script to analyze the results of the artificial reference genome mapping. This script identifies the putative TPs / FPs
on a specific data set (reads).
"""
parser = OptionParser(usage=usage,version="%prog 1.0")
parser.add_option("-p", "--phred", type='int',dest="phred",action="store", default=33,help="Specify the PHRED encoding of the input reads i.e. Illumina 1.3+ = -p 33.[default: %default]")
parser.add_option("-r", "--internalrank", type='int',dest="rank",action="store", default=1,help="Use internal ranking for reads (needed if the read names cannot be lexicographically be sorted in the same way in python and your OS by sam tools).[default: %default]")
parser.description=""
(options, args) = parser.parse_args()
numArgs = len(args)
# numArgs has to be equal to # required parameters
if numArgs ==2 :
input = args[0]
outpath = args[1]
# adjust options, otherwise default is used
internalrank = options.rank
phred = options.phred
else:
print("!!!Wrong number of parameters!!!")
parser.print_help()
sys.exit(1)
UseInternalRank = 1
controlDic = AM.readInput(input)
print("#######################################################################################")
print ("Input Settings:")
print ("Inputfile:\t%s" % input)
print ("Outputpath:\t%s" % outpath)
print ("Reads:\t%s" % controlDic["fastqfile"])
print ("UseInternalRank:\t%s" % UseInternalRank)
print ("Phred:\t%s" % phred)
print("#######################################################################################")
print ("\r\nStart...")
error = TestFiles(input)
if error == 1:
print "Program aborted. Check your inputfiles!"
sys.exit()
else:
print "Inputfiles checked successfully."
# check if output dir exists; else create output
if not os.path.exists(os.path.dirname(outpath)):
os.makedirs(os.path.dirname(outpath))
# readdictionary with ID as key and quality as VALUE
start1 = time.time()
readdic = AM.readReadQualities(controlDic["fastqfile"])
end1 = time.time()
# extend Readdic
readdic,reverseReadArray = AM.extendReadDic(readdic)
# init resultdictionary which carries all information about temporary results etc.
resultdic = {}
resultdic["nreads"] = len(readdic)
resultdic["maxalngt"] = 0
resultdic["AUC"] = {}
resultdic["alngts"] = {}
resultdic["id"] =[]
resultdic["mapper"] =[]
resultdic["lines"] = {}
resultdic["mreads"] = {} # mapped reads
# init list for comparing artificial and reference
compareList = []
#### looping #####
print ("########Progress (analysis):########")
print ("Mapper\t\tOutputfile\t\tOverallAlignments (TP/FP)\t\t Time")
for mapper in controlDic["mapper"].keys():
resultdic["mapper"].append(mapper)
# initialize output files (write once to them in case an earlier version exists)
AM.initOutFiles(controlDic,mapper,outpath)
# set refname
refname = controlDic["mapper"][mapper][0][0].split("/")[-1]
print ("%s" %mapper.upper()),
if UseInternalRank == 1:
rankDic = AM.GetOrderDictionary(controlDic["mapper"][mapper][0][0])
for p,artificial in enumerate(controlDic["mapper"][mapper][1]):
# list for calculation the difference between reference and artificial within a read region
compareList = AM.CreateCompareList(controlDic["fasta"]["reffasta"],controlDic["fasta"]["artfasta"][p])
filename = artificial.split("/")[-1]
outfile = mapper+"_"+artificial.split("/")[-1][:-4]+".esam"
start = time.time()
# main function, write results to 4 variables
tp,fp,LineCounter,MappedReads = AM.ReadSAMnoMem(controlDic["mapper"][mapper][0][0],artificial,outpath+outfile,compareList,readdic,rankDic,phred)
end = time.time()
# dummy, configurable sensitivity calculation
tempmaxsens = tp
# maintain results in the resultstructure dictionary
resultdic["alngts"][outfile] = (tp,fp)
resultdic["lines"][outfile] = LineCounter
resultdic["mreads"][outfile] = MappedReads
resultdic["id"].append(outfile)
print (outfile),
print ("\t%0.2f (%0.2f/%0.2f)" %(tp+fp,tp,fp)),
print ("\t%0.2f sec." %(end-start))
##################################################################################################################################
# start evaluation process. SAM files are not getting touched anymore
print ("\r\n########Progress (evaluation):########")
print ("Mapper\tTP\tFP\tTime")
for resultfile in resultdic["id"]:
print resultfile.split("_")[0],
#do sme name generation for this iteration
prefix = outpath+resultfile.split(".")[0]
filename = resultfile.split(".")[0]
# read data into an array
dataArray = AM.ReadFromTab(outpath+resultfile,resultdic["lines"][resultfile])
# get tp and fp arrays
tp =[i for i in dataArray if i.mr[0] ==1]
fp =[i for i in dataArray if i.mr[0] ==0]
print ("\t %d" % resultdic["alngts"][resultfile][0]), # TP
print ("\t %d" % resultdic["alngts"][resultfile][1]), # FP
start = time.time()
#try:
resultdic["AUC"][resultfile] = PD.CalculateRoc2(dataArray,prefix,resultdic["nreads"],resultdic["lines"][resultfile],resultdic["mreads"][resultfile],filename)
#except:
#print("error calculating AUC...")
end = time.time()
print ("\t%0.2f" %(end-start))
# prepare labels and names for plotting the data
ids =[i for i in resultdic['id']]
mappernames = [i for i in resultdic['mapper']]
label = [k for k in xrange(1,len(ids)+1)]
tuple =[resultdic['alngts'][k] for k in ids]
tp = [i[0] for i in tuple]
fp = [i[1] for i in tuple]
# plot an histogram: tp,fp comparison (all mappers included)
PD.plotOverviewHist(fp,tp,label,prefix,mappernames)
fobj = open(outpath+"results.txt","w")
## maintain resultfile
fobj.write("mapper\tfile\ttp\tfp\tsens\tspec\tAUC\tmappedReads\tInputReads\tF\r\n")
for resultfile in (resultdic["id"]):
mapper = resultfile.split("_")[0]
file =resultfile.replace(".esam","")
tp =resultdic["alngts"][resultfile][0]
fp = resultdic["alngts"][resultfile][1]
#sens = (tp / tp+fp) * F
sens = round((float(tp) / resultdic["lines"][resultfile]) * (float(resultdic["mreads"][resultfile] )/ resultdic["nreads"]),3)
#sens = 1-(fp / tp+fp) * F
spec = 1- round((float(fp) / resultdic["lines"][resultfile]) * (float(resultdic["mreads"][resultfile]) / resultdic["nreads"]),3)
auc = resultdic["AUC"][resultfile]
pmappedReads = round(float(resultdic["mreads"][resultfile]) / resultdic["nreads"],3)
fobj.write("%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\t%s\r\n" %(mapper,file,tp,fp,sens,spec,auc,resultdic["mreads"][resultfile],resultdic["nreads"],pmappedReads))
fobj.close()
if __name__ == "__main__":
a = time.time()
main()
b = time.time()
print ("Finished!")
print ("Done in %0.2f seconds!" % (b-a))
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